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Öğe Ace gene polymorphisms are ineffective on contrast induced nephropathy(Elsevier, 2022) Kilic, Ilhan; Palabiyik, Orkide; Taylan, Gokay; Sipahi, Tammam; Ustundag, SedatBackground: The renin-angiotensin system regulates the haemodynamics. ACE gene polymorphisms are known to influence serum angiotensin converting enzyme level. Contrast nephropathy develops after exposure to intravascular contrast media that influence vascular hemodynamics. ACE gene polymorpisms may have an enhancing role in contrast media related renal injury. The aim of the study: The aim of this study was to investigate the impact of ACE insertion/deletion (I/D) polymorphism in contrast-induced nephropathy (CIN) development. Methods: 194 patients with chronic kidney disease that were administered iodinated contrast media were examined. Patients were monitored for at least 7 days for CIN development after parenteral contrast exposure. Control and patient groups were divided in terms of CIN development status. Polymerase chain reaction was performed for the genotyping of the ACE gene polymorphism from DNAs that were isolated from peripheral blood of the patients. Results: 83 patients with CIN (34 women, 49 men) and 111 control patients without CIN (43 women, 68 men) were enrolled. Gender was not statistically different between the two groups (p = 0.75). The average age of the CIN group (71) was greater than that of the control group (68). No association was detected between ACE gene polymorphism (II, ID AND DD genotypes) and CIN in both patients and controls. Conclusion: ACE gene polymorphisms does not influence contrast induced nephropathy development.Öğe Alteration in cardiac PI3K/Akt/mTOR and ERK signaling pathways with the use of growth hormone and swimming, and the roles of miR21 and miR133(Spandidos Publ Ltd, 2019) Palabiyik, Orkide; Tastekln, Ebru; Doganlar, Zeynep Banu; Tayfur, Pinar; Dogan, Ayten; Vardar, Selma ArzuAthletes misuse recombinant human growth hormone (r-hGH) to enhance their performance. Although r-hGH is known to increase cardiac hypertrophy, the underlying molecular mechanism remains unclear. The aim of the present study was to investigate the role of r-hGH in cardiac intracellular signaling pathways and of miR-21 and miR-133 expression in rat hearts during exercise. A total of 36 adult male Sprague-Dawley rats were divided into sedentary control (SC, n=9), swimming exercise (SE, n=8), r-hGH (GH, n=10) and swimming exercise plus r-hGH (SE-GH, n=9) groups. The exercise groups completed a 1-h swimming exercise 5 times a week for 8 weeks. Subcutaneous r-hGH was administered as 0.3 mg/kg/day. Phosphoinositide-3-kinase (PI3K), serine/threonine protein kinase 1 (AKT1), extracellular signal-regulated kinase (ERK), microRNA (miR)-21 and miR-133 expression was evaluated in ventricular muscle by real-time quantitative polymerase chain reaction. Protein expression of PI3K, AKT1, ERK and mechanistic target of rapamycin (mTOR) was also assessed by immunohistochemistry. Statistical differences were analyzed by two-way ANOVA. PI3K and AKT1 expression and the gene and protein levels was notably increased in the SE-GH group compared with in SC ventricular tissues (P<0.05). mTOR protein expression was higher in the GH, SE and SE-GH groups compared with in the SC group (P<0.05, <0.05 and <0.001, respectively). ERK gene/protein expression was similar across all groups. miR-21 and miR-133 levels in ventricular muscle were higher in the SE and GH groups than those in the SC group. In summary, growth hormone application coupled with swimming exercise appeared to affect the PI3K/AKT/mTOR signaling pathway in the left ventricular tissue of rats; however, ERK signaling pathway appeared inactive in physiological left ventricular hypertrophy caused by swimming and GH administration over 8 weeks. Furthermore, GH treatment resulted in increased miR-21 and miR-133 expression. Future study by our group will aim to assess the effects of higher dose GH treatment.Öğe The alteration of NTproCNP plasma levels following anaerobic exercise in physically active young men(Turkish Soc Cardiology, 2015) Temur, Hilal Akseki; Vardar, Selma Arzu; Demir, Muzaffer; Palabiyik, Orkide; Karaca, Aziz; Guksu, Zuhal; Ortanca, ArifObjective: Amino-terminal propeptide of C-type natriuretic peptide (NTproCNP) is a synthesis product of C-type natriuretic peptide (CNP). In this study, plasma levels of NTproCNP were compared before and after exercise in healthy young subjects who are physically active (PA) or not physically active (NPA). Methods: The study was carried on PA group (n=10) who defined the exercise duration more than 2.5 hours per week for at least one year and NPA group (n=10) whose exercise duration was lower than 1.5 hours per week. The level of maximal oxygen consumption was determined. Wingate exercise test was applied on the following day. Plasma NTproCNP levels were measured before the exercise and at the 1st, 5th and 30th minute after the exercise. Results: Exercise duration of physically active group was reported as 11.3 +/- 5.0 hours per week. Basal NTproCNP levels of the groups were found to be comparable. NTproCNP levels in the 5th minute (0.93 +/- 0.23 pmol/L; p<0.05) and in the 30th minute (0.77 +/- 0.21 pmol/L p<0.05) after exercise were higher than the levels before exercise (0.64 +/- 0.29 pmol/L) in PA group. Additionally, the plasma levels of NTproCNP after 5th minute of exercise were higher in PA group (0.93 +/- 0.23 pmol/L) than NPA group (0.74 +/- 0.16 pmol/L, p<0.05). Conclusion: Being physically active may be a fact affecting the secretion of CNP, which plays a protective role in endothelium, following exercise.Öğe Apical aneurysm or transient apical ballooning? Potential dilemma in risk stratification of hypertrophic cardiomyopathy(Bmj Publishing Group, 2020) Yalta, Kenan; Gurdogan, Muhammet; Palabiyik, Orkide[Abstract Not Available]Öğe Association of Angiotensin II Type 1 Receptor A1166C Gene Polymorphisms with Coronary Artery Disease in Thrace Region of Turkey(Erciyes Univ Sch Medicine, 2021) Taylan, Gokay; Palabiyik, Orkide; Ozkalayci, Flora; Yilmaztepe, Mustafa Adem; Sivri, Nasir; Aksoy, YukselObjective: Although the risk factors for coronary artery disease (CAD) are well established, a significant gap still exists in understanding the pathology of atherosclerotic heart disease evolving without conventional risk factors. Therefore, genetic factors are considered to play a significant role in this setting. The present study aimed to assess the relationship between angiotensin 2 type 1 receptor (AT1R) A1166C gene polymorphism and CAD. Materials and Methods: Patients with documented CAD (n=121) were compared with controls with normal coronary arteries (n=121). CAD was diagnosed using a coronary angiography. The median age of participants was 59 +/- 12 years with an equal sex distribution. A comparison between the two groups with regard to the AT1R A1166C gene polymorphism was made through the amplification of DNA using polymerase chain reaction. Results: This study demonstrated that adenine-adenine and cytosine-cytosine (CC) genotypes were more frequent, yet adenine-cytosine genotype was less frequent among patients with CAD compared with controls [p=0.003), 95% confidence interval (CI)]. The AT1R A1166C gene polymorphism along with the CC genotype and C allele was found to be associated with CAD. Further, gender, hypertension, family history, age, and low levels of serum high-density lipoprotein also had a significant relationship with AT1R A1166C gene polymorphism. Conclusion: The present study suggested AT1R A1166C gene polymorphism, CC genotype, and C allele as potential risk factors for atherosclerotic CAD. Patients harboring these genetic variants should be under close supervision for the development of CAD.Öğe Cardiac Biomarkers in the Setting of Asthma Exacerbations: a Review of Clinical Implications and Practical Considerations(Current Medicine Group, 2020) Yalta, Kenan; Yalta, Tulin; Gurdogan, Muhammet; Palabiyik, Orkide; Yetkin, ErtanPurpose of Review The present paper aims to highlight clinical implications of elevated cardiac biomarkers and associated myocardial dysfunction in a variety of cardiac and non-cardiac scenarios in patients with an asthma exacerbation, and to propose a basic algorithm for cardiovascular evaluation and triage (and hence, for further management) of these patients primarily based on evaluation of cardiac biomarkers along with basic diagnostic modalities and specific cardiac symptoms in the hospital setting. Recent Findings Elevation of cardiac biomarkers in the setting of an asthma exacerbation mostly signifies a new-onset subclinical myocardial dysfunction/injury generally associated with certain asthma-related factors including acute hypoxemia and bronchodilator therapy, and usually has a limited prognostic value in these patients. On the other hand, elevation of these biomarkers in patients with an asthma exacerbation might also denote a variety of certain life-threatening cardiac or non-cardiac conditions associated with significant myocardial dysfunction (acute coronary syndromes (ACSs), sepsis, etc.) that might be masked by the rampant course of the asthma exacerbation, and hence, might possibly go undetected potentially aggravating the prognosis in a portion of these patients. In patients with an asthma exacerbation, it seems imperative to timely diagnose and manage emerging diverse clinical conditions particularly through the guidance of cardiac biomarkers and associated myocardial dysfunction patterns in an effort to improve overall prognosis in these patients.Öğe Comparison of serum BDNF, IL-1?, IL-6, TNF-?, CRP and leucocyte levels in unipolar mania and bipolar disorder(Cambridge Univ Press, 2021) Gorgulu, Yasemin; Uluturk, Milkibar Kyazim; Palabiyik, OrkideObjective: Unipolar mania is not included in the diagnostic and statistical manual of mental disorders-5 (DSM-5) as a separate diagnosis, although it is defined by widely accepted diagnostic criteria. The aim of this study was to investigate the differences between unipolar mania and bipolar disorder in terms of clinical and inflammatory parameters. Methods: The data of 495 hospitalised patients with bipolar disorder diagnoses were analysed retrospectively. Forty met the diagnostic criteria for unipolar mania. Two patients refused to participate in the study. Thirty-eight unipolar mania patients and 42 randomly selected patients with bipolar disorder diagnosis were included in the study. The two groups were compared in terms of sociodemographic, clinical characteristics, serum brain-derived neurotrophic factor, C-reactive protein (CRP), leucocyte and cytokine levels. Results: A total of 40 (8.08%) of 495 patients diagnosed with bipolar disorder met the unipolar mania diagnostic criteria. The number of manic episodes and the number of hospitalisations were statistically higher in the unipolar mania group than in the bipolar disorder group. Among all the manic symptoms, the incidence of symptoms such as euphoria, increased sexual interest, grandiosity and delusions were found to be statistically higher in the unipolar mania group. Interleukin (IL)-6 and CRP levels were significantly higher in the unipolar mania group than in the bipolar disorder group. Conclusion: Unipolar mania differs from bipolar disorder in terms of clinical features and serum IL-6 and CRP levels.Öğe The Effect of a High-Protein Diet and Exercise on Cardiac AQP7 and GLUT4 Gene Expression(Springer/Plenum Publishers, 2016) Palabiyik, Orkide; Karaca, Aziz; Tastekin, Ebru; Yamasan, Bilge Eren; Tokuc, Burcu; Sipahi, Tammam; Vardar, Selma ArzuHigh-protein (HP) diets are commonly consumed by athletes despite their potential health hazard, which is postulated to enforce a negative effect on bone and renal health. However, its effects on heart have not been known yet. Aquaporin-7 (AQP7) is an aquaglyceroporin that facilitates glycerol and water transport. Glycerol is an important cardiac energy production substrate, especially during exercise, in conjunction with fatty acids and glucose. Glucose transporter 4 (GLUT4) is an insulin-sensitive glucose transporter in heart. We aimed to investigate the effect of HPD on AQP7 and GLUT4 levels in the rat heart subjected to exercise. Male Sprague-Dawley rats were divided into control (n = 12), exercise (E) training (n = 10), HPD (n = 12), and HPD-E training (n = 9) groups. The HPD groups were fed a 45 % protein-containing diet 5 weeks. The HPD-E and E groups were performed the treadmill exercise during the 5-week study period. Real-time polymerase chain reaction and immunohistochemistry techniques were used to determine the gene expression and localization of AQP7 and GLUT4 in heart tissue. Results of relative gene expression were calculated by the 'Pfaffl' mathematical method using the REST program. Differences in AQP7 and GLUT4 gene expression were expressed as fold change compared to the control group. Heart weight/tibia ratio and ventricular wall thickness were evaluated as markers of cardiac hypertrophy. Further, serum glucose, glycerol, and insulin levels were also measured. AQP7 gene expression was found to be increased in the E (3.47-fold, p < 0.001), HPD (5.59-fold, p < 0.001), and HPD-E (3.87-fold, p < 0.001) groups compared to the control group. AQP7 protein expression was also increased in the HPD and HPD-E groups (p < 0.001). Additionally, cardiac mRNA expression levels of GLUT4 showed a significant increase in the E (2.16-fold, p < 0.003), HPD (7.14-fold, p < 0.001), and HPD-E (3.43-fold, p < 0.001) groups compared to the control group. GLUT4 protein expression was significantly increased in the E, HPD, and HPD-E groups compared to the control group (p = 0.024, p < 0.001, and p < 0.001, respectively). Furthermore, Serum glucose levels were significantly different between groups (p < 0.005). This difference was observed between the HPD groups and normal-protein diet groups (C and E). Serum insulin levels were higher for HPD groups compared with the normal-protein diet groups (p < 0.001), whereas no differences were observed between the exercise and sedentary groups (p = 0.111). Serum glycerol levels were significantly increased in the HPD groups compared with control and E groups (p < 0.05 and p < 0.05, respectively). Consumption of HPD supplementation caused the increased effects on AQP7 and GLUT4 expression in rat heart.Öğe The Effect of Fructose Administration on Myocardial Infarct Area and Hemodynamic Responses in Ischemia-Reperfusion Model in Isolated Rat(Wiley, 2022) Palabiyik, Orkide; Aydin, Muhammed Ali; Deger, Ecem Busra; Korkmaz, Selcuk; Vardar, Selma Arzu[Abstract Not Available]Öğe The effect of fructose rich diet and voluntary physical activity on cardiac hemodynamic responses during ischemia and reperfusion of isolated rat heart(Wiley-Blackwell, 2016) Vardar, Selma Arzu; Tayfur, Pinar; Palabiyik, Orkide; Tastekin, Ebru; Uzun, Nursen; Sut, Necdet[Abstract Not Available]Öğe The effect of growth hormone and/or swimming exercise on PI3K, AKT, PTEN and miR21 expressions in rats(Wiley-Blackwell, 2016) Palabiyik, Orkide; Tastekin, Ebru; Doganlar, Zeynep Banu; Tayfur, Pinar; Vardar, Selma Arzu[Abstract Not Available]Öğe The effect of high protein diet and exercise on irisin, eNOS, and iNOS expressions in kidney(Taylor & Francis Ltd, 2016) Tastekin, Ebru; Palabiyik, Orkide; Ulucam, Enis; Uzgur, Selda; Karaca, Aziz; Vardar, Selma Arzu; Yilmaz, AliLong-term effects of high protein diets (HPDs) on kidneys are still not sufficiently studied. Irisin which increases oxygen consumption and thermogenesis in white fat cells was shown in skeletal muscles and many tissues. Nitric oxide synthases (NOS) are a family of enzymes catalyzing the production of nitric oxide (NO) from L-arginine. We aimed to investigate the effects of HPD, irisin and NO expression in kidney and relation of them with exercise and among themselves. Animals were grouped as control, exercise, HPD and exercise combined with HPD (exercise-HPD). Rats were kept on a HPD for 5 weeks and an exercise program was given them as 5 exercise and 2 rest days per week exercising on a treadmill with increasing speed and angle. In our study, while HPD group had similar total antioxidant capacity (TAC) levels with control group, exercise and exercise-HPD groups had lower levels (p<0.05). Kidneys of exercising rats had no change in irisin or eNOS expression but their iNOS expression had increased (p<0.001). HPD-E group has not been observed to cause kidney damage and not have a significant effect on rat kidney irisin, eNOS, or iNOS expression. Localization of irisin, eNOS, and iNOS staining in kidney is highly selective and quite clear in this study. Effects of exercise and HPD on kidney should be evaluated with different exercise protocols and contents of the diet. risin, eNOS, and iNOS staining localizations should be supported with various research studies.Öğe The Effect of Isatin on Cardiac Hemodynamic Function in Physically Active Rats(Wiley, 2023) Vardar, Selma Arzu; Aydin, Muhammed Ali; Palabiyik, Orkide; Deger, Ecem Busra; Tosunoglu, Esra Akbas; Firat, Nihayet; Sut, Necdet[Abstract Not Available]Öğe The effect of subthreshold depressive symptoms on cognitive functions and peripheral biomarkers in bipolar disorder(Taylor & Francis Ltd, 2023) Sogut, Kubra; Gorgulu, Yasemin; Palabiyik, OrkideObjective: A global approach to factors responsible for functional impairment in patients with BD is necessary.Method: Ninety-three euthymic patients with BD [49 patients with SD (subthreshold depression) and 44 patients without SD] and 48 healthy controls were invited for evaluation of demographic, clinical, and cognitive characteristics. To define SD, the lower limit was HDRS =4 points and the upper limit was HDRS <9 points. Stroop test, California verbal learning test, digit-span test, controlled word association test, and clock drawing test were performed. Serum BDNF levels were measured. Additionally in the BD group; blood drug (lithium, valproic acid), leukocyte, C-reactive protein (CRP), TSH, and vitamin B12 levels were measured.Results: We found no difference between serum BDNF levels of BD (n = 93) and controls. The cognitive performances of the BD group were worse than the control group (p < 0.001). Attention, working memory, and stroop performance of patients with SD were worse than patients without SD (p < 0.05). Verbal fluency, stroop test, and planning performance decreased as serum CRP level increased in patients with BD (p < 0.05).Conclusions: Although the patient group with SD was in euthymia, their cognitive performance was worse than the group without SD. Poor cognitive performance in BD was associated with serum CRP levels.Öğe The effect of water loading on serum copeptin and aquaporin 2 levels in overweight and obese individuals(Wiley, 2023) Yanik, Serap; Palabiyik, Orkide; Deger, Ecem Busra; Korkmaz, Selcuk[Abstract Not Available]Öğe Effects of high fructose diet and exercise on glucose transporter 5 and putative glycerol-transporter aquaporin 7 in the in vivo rat heart(Wiley-Blackwell, 2015) Karaca, Aziz; Vardar, Selma Arzu; Palabiyik, Orkide; Tastekin, Ebru; Turan, Fatma Nesrin[Abstract Not Available]Öğe Effects of Physical Activity on Metabolic and Cardiac Hemodynamic Alterations Related with Fructose-rich Diet(Wiley-Blackwell, 2016) Tayfur, Pinar; Palabiyik, Orkide; Uzun, Nursen; Sut, Necdet; Vardar, Selma Arzu[Abstract Not Available]Öğe Endothelial Nitric Oxide Synthase and Angiotensin Converting Enzyme Gene Polymorphisms in Migraine Patients(Turkish Neuropsychiatry Assoc-Turk Noropsikiyatri Dernegi, 2013) Sipahi, Tammam; Guldiken, Baburhan; Kabayel, Levent; Palabiyik, Orkide; Ozkan, Hulya; Okman Kilic, Tulay; Sut, NecdetIntroduction: In this study, we investigated the association of migraine with the Variable Number of Tandem Repeats (VNTR), repeated as 27 base pair, gene polymorphism in intron 4 of the endothelial nitric oxide synthase (eNOS) and the insertion/deletion of angiotensin converting enzyme (ACE) gene polymorphisms. Methods: One hundred and five migraine and ninety seven healthy female control subjects were enrolled in the study. The patients were subdivided as migraine with aura and without aura, and the frequency and severity of migraine headaches were recorded. The eNOS VNTR (eNOS 4 a/b) and ACE insertion/deletion gene polymorphisms (ACE I/D) were assessed by polymerase chain reactions. Results: The allele and genotype frequencies of eNOS 4 a/b gene polymorphism showed no difference between the migraine and control groups. The genotypic distribution of the ACE I/D gene polymorphism in the migraine group significantly differed from that in the control group. The DD and ID genotype increased the risk of migraine as much as 2.571 (95% CI-1.138-5.811) and 4.453 (95% CI-2.006-9.883) compared to the II genotype. The same increased risk sustained for both genotypes in the migraine with aura subgroup, but only the ID genotype remained as the risk factor in the migraine without aura subgroup (OR-3.750, 95% CI-1.493-9.420). No association of gene polymorphisms with migraine frequency and severity was observed. Conclusion: Our findings support the relationship between migraine and the ACE I/D gene polymorphism. However, no association was found between migraine and the eNOS 4 a/b gene polymorphism.Öğe Growth Hormone Affects PI3K/AKT/mTOR Signaling Pathways in the Rat Heart Subjected to Exercise(Wiley-Blackwell, 2016) Palabiyik, Orkide; Tayfur, Pinar; Tastekin, Ebru; Doganlar, Zeynep Banu; Vardar, Selma Arzu[Abstract Not Available]Öğe Hemodynamic effects of atrial natriuretic peptide in ischemia-reperfusion injury that occurs after exercise(Tubitak Scientific & Technological Research Council Turkey, 2015) Vardar, Selma Arzu; Palabiyik, Orkide; Topuz, Ruhan Deniz; Gurel, Elif Ezgi; Caliskan, Semra; Topcu Ozen, Serap; Sut, NecdetBackground/aim: Atrial natriuretic peptide (ANP) is known as a protective agent against ischemia-reperfusion injury for cardiomyocytes. We compared the hemodynamic effects of ANP and isatin, which is known as an ANP receptor blocker, in ischemia followed by reperfusion in exercised rat hearts with nonexercised ones. Materials and methods: Isolated hearts were perfused in 4 exercised (E) groups after a running protocol for 5 days and 4 nonexercised (NE) groups. In the first protocol, ANP was added to the perfusion solution before ischemia in an E and NE group. In the second protocol, different doses of isatin (0.1, 10, 100 mu M/L) were added to the perfusion solution before ANP in 3 E and 3 NE groups. Left ventricular developed pressure (LVDP) and maximum and minimum rates of change in left ventricular pressure (dP/dtmax and dP/dtmin) were recorded. Results: Higher LVDP and dP/dtmin values were observed in the E group than the NE group following addition of ANP before ischemia. Values of dP/dtmax were higher in the E group at the first minute of reperfusion period. Hemodynamic difference was not observed between groups given the same amount of isatin before ANP. Conclusion: This study indicated that higher ANP concentrations before ischemia were more effective on the left ventricle contractility and relaxation functions in the hearts that were exposed to exercise.
