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Öğe Diversity analysis of the rice cultivars (Oryza sativa L.) by utilizing SSRs rice diversity by SSRs(Wiley, 2021) Beser, Necmi; Mutafcilar, Zeynep Cisem; Hasancebi, SemraThis study was carried out to analysis of genetic diversity of rice marketed in Turkey. Sixty rice varieties used as a material. A total of 50 SSR markers distributed to all 12 rice chromosomes were used for the diversity analysis of the genotypes. Out of these, 43 SSRs produced clear, distinct polymorphic bands among the rice varieties, and a total of 227 alleles were obtained. The number of alleles per locus ranged from 9 to 1. The mean number of alleles per locus was 5.25, and 22 markers (51%) had 6 or more alleles. Clustering of 60 varieties based on 43 SSR markers was carried out. While aromatic varieties were placed into an out-group Rocca and Baldo which are parents of many rice varieties and 47 varieties were placed into the same clusters. This SSR polymorphism analysis revealed genomic relationships in rice marketed in Turkey. Practical applications Results of this study could be used to diversity analysis of rice and other crops and rice breeding programs. It could be used to study genetic diversity of polished rice at the market and consumer preference by countries.Öğe IDENTIFICATION AND PRODUCTION OF PHENOLIC NICOTIFLORIN IN ASTRAGALUS CHRYSOCHLORUS CALLUS(Soc Stiinte Farmaceutice Romania, 2018) Turgut-Kara, Neslihan; Cakir, Ozgur; Hasancebi, Semra; Karabey, Fatih; Ari, SuleAstragalus chrysochlorus Boiss. & Kotschy (2n = 16) is one of the rare Turkish endemic species and it is listed in the Red Data Book of Turkish Plants as endangered. This species has been used traditionally for its wound healing properties and a crude ethanol extract prepared from the roots exhibits antioxidant and cytotoxic activities. In this study, a detailed phytochemical analysis was performed on A. chrysochlorus calli that resulted in the isolation of a major constituent. The purified molecule's structure elucidation was completed by spectral methods [nuclear magnetic resonance (NMR) and mass spectrometry (MS)], which revealed a rarely encountered the flavonoid in the Astragalus genus, nicotiflorin. In order to increasing nicotiflorin content in the callus cultures, the effects of culturing time and elicitor treatment were investigated. The HPLC analyses showed that the maximal production of nicotiflorin occurred with long-term cultured (13 years old) callus as 4775 mu g/g dry weight (DW), whereas it was 132 mu g/g DW for short-term cultured (2 months old) ones. Then, the 24 h treatment of the yeast extract that was used as biotic elicitor had negative effect on the production of nicotiflorin. The data obtained from this study could be significant for the mass production of nicotiflorin from long term in vitro cultured A. chrysochlorus callus.Öğe IDENTIFICATION OF LENS CULTIVARS IN MARKET BY MOLECULAR TOOLS: DNA BARCODING AND SSRs(Trakya Univ Balkan Yerlesesi Enstituler Binasi, 2023) Tatlises, Metin Burak; Hasancebi, SemraSubstitution of plant cultivars of high commercial value with a cheaper, lower quality one is a common fraud committed against consumers and producers. Since it is one of the most widely grown legumes, lentil (Lens culinaris Medik.) is suitable for such frauds. This study aimed to identify lentil cultivars which are registered and authorized in the market in Turkiye by using current molecular methods. For this purpose, 26 lentil cultivars were analyzed for 15 SSR markers and two DNA barcode regions (trnH-psbA and matK). A high allele diversity was observed by 12 scorable SSR markers, and the average number of alleles was determined to be 16. One of the important findings was the presence of cultivar-specific alleles that can be used to identify each cultivar in the lentil market in Turkiye. At least one cultivar-specific allele was obtained for each cultivar. The lentil cultivars were also analyzed by two DNA barcode regions as trnH-psbA and matK. While it was observed that the rate of the intra-species variation for the trnH-psbA region was low and 26 varieties were divided into 7 groups, higher rate was found for matK and samples were distributed into 14 groups. Nevertheless, it was observed that intra-species discrimination can be made more effective when both loci are used together and 26 species were distributed into 18 different groups. We expect that the results of this study, especially the cultivar-specific SSR alleles and DNA barcoding sequence data may be used routinely to identify production and packaged products that are commercially available in markets.Öğe Morphological and molecular evaluation of Turkish rice (Oryza sativa L.) landraces(Springer, 2021) Konak, Mete Arslan; Hasancebi, Semra; Beser, NecmiThis study was carried out to evaluate Turkish rice landraces, in 2016 and 2017. Twenty-nine morphological traits were used for morphological evolution and 10 SSR markers were used for molecular evolution in 27 varieties. Based on morphological dendrogram, the landraces were divided in to 11 groups at 5 level differences. It was found that all 27 landraces had absent or very weak pubescence of panultimate leaf blade, broad decorated seed width and non-waxy kernel, however, they were highly polymorphic for the other 26 characters studied. In total 51 alleles were produced by screening with SSRs and among the markers; RM552 and RM287 were highly polymorphic for Turkish rice landraces with 11 and 8 alleles respectively. Average allele number was 5.1 and PIC ranged from 0.36 to 0.84. The UPGMA cluster dendrogram generated by using SSRs information and cluster grouped the 27 landraces in 2 major clusters. A significant level of polymorphism on molecular levels was observed. The study shows that some landraces with same local name were very distant from each other, while some local varieties with different names were same landraces.Öğe Proteome Profiling by 2D-Liquid Chromatography Method for Wheat-Rust Interaction(Humana Press Inc, 2017) Hasancebi, SemraWheat-rust interactions are extremely complex biological processes which are accompanied with the defense/attack responses to survive and overcome pathogen attack or plant defense. Understanding of molecular mechanism of these interactions is a promising way to develop sustainable combat. Therefore, many studies have been performed to reveal the active host and pathogen-derived genes and their products during the infection or defense using different approaches for many decades. Particularly proteomics technology and proteome profiling which is a large scale analysis of a protein mixture to reveal differently expressed proteins under a certain conditions has become a very important tool for providing real insights into the extremely complex interactions. Moreover, this type of research has the potential to explore target proteins/genes such as effectors that can be used in disease management strategies. Hence, in this chapter we describe the proteome profiling protocols by using 2D-LC system.
