Proteome Profiling by 2D-Liquid Chromatography Method for Wheat-Rust Interaction

Küçük Resim Yok

Tarih

2017

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Humana Press Inc

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

Wheat-rust interactions are extremely complex biological processes which are accompanied with the defense/attack responses to survive and overcome pathogen attack or plant defense. Understanding of molecular mechanism of these interactions is a promising way to develop sustainable combat. Therefore, many studies have been performed to reveal the active host and pathogen-derived genes and their products during the infection or defense using different approaches for many decades. Particularly proteomics technology and proteome profiling which is a large scale analysis of a protein mixture to reveal differently expressed proteins under a certain conditions has become a very important tool for providing real insights into the extremely complex interactions. Moreover, this type of research has the potential to explore target proteins/genes such as effectors that can be used in disease management strategies. Hence, in this chapter we describe the proteome profiling protocols by using 2D-LC system.

Açıklama

Anahtar Kelimeler

Proteomics, Proteome Profiling, Plant-Pathogen Interaction, Defense, 2D-LC, PF2D, Leaf Protein Extraction, Wheat, Stripe Rust, 2-Dimensional Liquid-Chromatography, Fractionation

Kaynak

Wheat Rust Diseases: Methods And Protocols

WoS Q Değeri

N/A

Scopus Q Değeri

Q3

Cilt

1659

Sayı

Künye