IDENTIFICATION OF LENS CULTIVARS IN MARKET BY MOLECULAR TOOLS: DNA BARCODING AND SSRs
Küçük Resim Yok
Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Trakya Univ Balkan Yerlesesi Enstituler Binasi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Substitution of plant cultivars of high commercial value with a cheaper, lower quality one is a common fraud committed against consumers and producers. Since it is one of the most widely grown legumes, lentil (Lens culinaris Medik.) is suitable for such frauds. This study aimed to identify lentil cultivars which are registered and authorized in the market in Turkiye by using current molecular methods. For this purpose, 26 lentil cultivars were analyzed for 15 SSR markers and two DNA barcode regions (trnH-psbA and matK). A high allele diversity was observed by 12 scorable SSR markers, and the average number of alleles was determined to be 16. One of the important findings was the presence of cultivar-specific alleles that can be used to identify each cultivar in the lentil market in Turkiye. At least one cultivar-specific allele was obtained for each cultivar. The lentil cultivars were also analyzed by two DNA barcode regions as trnH-psbA and matK. While it was observed that the rate of the intra-species variation for the trnH-psbA region was low and 26 varieties were divided into 7 groups, higher rate was found for matK and samples were distributed into 14 groups. Nevertheless, it was observed that intra-species discrimination can be made more effective when both loci are used together and 26 species were distributed into 18 different groups. We expect that the results of this study, especially the cultivar-specific SSR alleles and DNA barcoding sequence data may be used routinely to identify production and packaged products that are commercially available in markets.
Açıklama
Anahtar Kelimeler
Food Traceability, DNA Barcoding, SSR, Lens Culinaris, Microsatellite, Adulteration, Regions, Hrm, Authentication, Sequences, Tortoise, Utility
Kaynak
Trakya University Journal Of Natural Sciences
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
24
Sayı
2
