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Öğe Can obstructive apnea and hypopnea during sleep be differentiated by using electroencephalographic frequency bands? Statistical analysis of receiver-operator curve characteristics(Tubitak Scientific & Technological Research Council Turkey, 2011) Ucar, Erdem; Sut, Necdet; Gulyasar, Tevfik; Umut, Ilhan; Ozturk, LeventAim: To investigate whether electroencephalographic (EEG) frequency bands are applicable in distinguishing abnormal respiratory events such as obstructive apnea and hypopnea in patients with sleep apnea. Materials and methods: The polysomnographic recordings of 20 patients were examined retrospectively. EEG record segments were taken from C4-A1 and C3-A2 channels and were analyzed with software that uses digital signal processing methods, developed by the study team. Percentage values of delta, theta, alpha, and beta frequency bands were evaluated through discriminant and receiver-operator curve (ROC) analysis to distinguish between apneas and hypopneas. Results: For the G4-A1 channel, delta (%) provided the highest discriminative value (AUG = 0.563; P < 0.001); on the other hand, alpha (%) gave the lowest discriminative value (AUG = 0.519; P = 0.041). Likewise, whereas for the C3-A2 channel delta (%) gave the highest discriminative value (AUG = 0.565; P < 0.001), alpha produced the lowest discriminative value (AUG = 0.501; P = 0.943). Conclusion: As a result of discriminant analysis, the accurate classification rate of hypopneas was 44.8% and the accurate classification of obstructive apneas was 63.5%. Of the 4 frequency bands, the most significant was delta. The predictive values were not at significance level.Öğe Comparison of Before and After Varicocelectomy Levels of Trace Elements, Nitric Oxide, Asymmetric Dimethylarginine and Malondialdehyde in the Seminal Plasma and Peripheral and Spermatic Veins(Humana Press Inc, 2015) Kiziler, Ali Riza; Aydemir, Birsen; Guzel, Savas; Yazici, Cenk Murat; Gulyasar, Tevfik; Malkoc, Ercan; Acar, AyseIncreased oxidative damage has been suggested to play an important role in the spermatogenesis and sperm function changes in patients with varicocele. However, changes in levels of nitric oxide (NO), asymmetric dimethylarginine (ADMA), malondialdehyde (MDA), iron (Fe), zinc (Zn) and copper (Cu) in blood and seminal plasma, and semen quality, are unknown. The aim of this study was to investigate the NO, ADMA, Fe, Cu, Zn and MDA levels from seminal plasma and peripheral and spermatic vein blood samples of patients with varicocele before and after varicocelectomy. In this before and after comparative study, 29 consecutive patients attending a training hospital in Tekirdag, Turkey, were recruited. MDA and NO levels were determined by spectrophotometric methods. The levels of ADMA were determined by enzyme-linked immunosorbent assay method. Trace element level was determined by atomic absorption spectrophotometric method. The levels of MDA in the seminal plasma and peripheral and spermatic vein samples were observed to decrease significantly in the comparison of before and after phases of the study group (p= 0.022, p= 0.001 and p= 0.034, respectively). Also, the levels of NO in the seminal plasma and spermatic vein samples decreased significantly in the comparison of before and after phases of the study group (p= 0.025 and p= 0.001, respectively), while the levels of ADMA in seminal plasma and spermatic vein samples increased significantly in the comparison of before and after phases of the study group (p= 0.003 and p= 0.001, respectively). There were no significant differences in the levels of trace elements and sperm count (p> 0.05). Oxidative stress is significantly higher in the spermatic vein and seminal plasma samples of patients with varicocele before varicocelectomy. In conclusion, these events may be evaluated accordingly for the potentially beneficial treatment methods.Öğe Comparison of Mineral Levels in Bone and Blood Serum of Cattle in Northwestern Turkey(Medwell Online, 2009) Gonul, Remzi; Kayar, Abdullah; Bilal, Tanay; Erman, O. R. M.; Parkan, D. V. M. Cagla; Dodurka, H. Tamer; Gulyasar, TevfikIn order for the mineral levels in the body to be accurately determined, it is essential to determine the mineral content of bones together with that of blood serum. In this study, blood serum and bone samples were collected from cattle in and around the provinces of Istanbul, Tekirdag, Kirklareli and Edirne and it was attempted to determine reference values. In each region, blood and bone samples were collected from 100 heads of cattle and Phosphorus. (P), Calcium (Ca) and Magnesium (Mg) proportions were calculated. According to the results, it was found that the levels of P, Ca and Mg in bone samples of the cattle in Northwest of Turkey were 132.76 +/- 17.84, 333.23 +/- 163.02 and 2.38 +/- 0.32 mg g(-1), respectively, while mineral levels in blood serum were 7.88 +/- 1.05, 10.80 +/- 0.71 and 2.37 +/- 0.68 mg dL(-1), in the same order. It was thought that these values might constitute a reference point for serial measurements that would be made for keeping the cattle in our region under control against metabolic diseases.Öğe CYCLIN D1 A870G POLYMORPHISM AND PROGNOSIS OF NON-SMALL CELL LUNG CANCER(Lippincott Williams & Wilkins, 2011) Kocak, Zafer; Ozen, Alaattin; Cakina, Suat; Saynak, Mert; Gulyasar, Tevfik; Sipahi, Tammam[Abstract Not Available]Öğe Doublecortin-like kinase 1 levels and oxidant status in Alzheimer's disease(Cukurova Univ, Fac Medicine, 2017) Guzel, Savas; Yildiz, Ozlem; Unal, Aysun; Kiziler, Ali Riza; Gulyasar, Tevfik; Guzel, Eda Celik; Fidan, CigdemPurpose: Alzheimer disease (AD) is a progressive neurodegenerative disease that affects the neurons in various parts of the central nervous system. Recently discovered protein Doublecort like kinase-1 (DCLK-1) is one of the microtubule-associated protein. Our goal is to investigate the relationship of the role of the DCLK-1 in AD disease and oxidative stress. Material and Methods: The study included Alzheimerdisease- diagnosed 60 patients admitted to the clinic with memory disorders, and 30 healthy subjects. In the serum of patient and control group, DCLK-1, tau protein and zinc levels were measured. To assess the presence of oxidative stress, malondialdehyde (MDA), protein carbonyl group (PCG), protein thiol groups (PTG), glutathione (GSH) and catalase levels were detected. Dementia level was staged with Mini-Mental State Examination (MMSE) and Dementia Clinical Staging Scale (CDR). Results: Serum DCLK-1 and tau levels were determined significantly higher in AD compared to the control group). In the group with AD, levels of MDA, and PCG levels were significantly higher and GSH, catalase levels were determined significantly lower. DCLK-1 and MDA levels were determined significantly higher in the group with severe AD compared to the group with mild AD. In AD group, a positive correlation between DCLK-1 and, CDR and MDA; and negative correlation was found between MMSE and B12 vitamin Conclusions: The presence of a relation with increase in DCLK1 levels in AD and risk factors shows that it can be a new marker in assessing the disease.Öğe Effects of isoxsuprine hydrochloride on electrocardiographic and trace element status in sheeps(Pakistan Medical Assoc, 2016) Vural, Handan Aydin; Koenhemsi, Lora; Gonul, Remzi; Yardibi, Hasret Demircan; Gulyasar, Tevfik; Or, Erman; Hosturk, Gulhan TurkayObjective: To study the effect of isoxsuprine hydrochloride on the ischaemic electrocardiographic change and trace element status in sheep. Methods: This study was conducted from March 16 to 23, 2012, at Istanbul University, Turkey, and comprised sheep aged 6 months. The animals were divided into two equal groups. The control group was fed a standard diet and had free access to water. In the experimental group, isoxsuprine hydrochloride was injected at a dose of 0.6 mg/kg through the intramuscular route. Electrocardiographic changes, including creatine kinase and cardiac troponin-I, and serum levels of selenium, copper, calcium, magnesium, iron and zinc were investigated in healthy sheep. SPSS 15 was used for statistical analysis. Results: The 14 sheep were divided into two groups of 7(50%) each. The overall mean weight of the study population was 35 +/- 10kg. Selenium, calcium, iron and zinc concentrations did not show any difference in serum samples (p > 0.05). However, copper and magnesium concentrations decreased in serum after the administration of the drug (p < 0.05). In the experimental group, ST segment depression and abnormal T-wave was found in 6(86%) animals within 60 min. Conclusion: Isoxsuprine hydrochloride increased cardiotoxicity risk in sheep.Öğe THE EVALUATION OF RELATIONSHIP BETWEEN ERYTHROCYTE AND SERUM FERRITIN LEVELS WITH AMINOTRANSFERASE ENZYME LEVELS IN ANTI-HCV POSITIVE HEMODIALYSIS PATIENTS(Istanbul Univ, Faculty Medicine, Publishing Office, 2008) Magin, Hasan; Seymen, Pinar; Aydemir, Birsen; Gulyasar, Tevfik; Seymen, H. Oktay; Demirtunc, RefikObjective: Serum ferritine level which is released from hepatocellular stores due to chronic inflammation of HCV infection, is accepted as an independent indicator of hepatocellular damage. It is not known whether intra- erythrocyte ferritin could be used as an indicator of hepatocellular damage as with serum ferritin, AST and ALT. In our study, we evaluated the relationship between aminotransferase level with eriythrocyte ferritin level and serum ferritin level in anti- HCV positive hemodialysis patients. Materials and methods: 40 HCV patients, taking hemodalysis therapy at five different hemodialysis center, were included in the study. They had no active inflamation, malignity and policytemia and had not been given blood transfusion. Erythrocyte and serum ferritin, ALT, AST, CRP, HBs- Ag, anti- HCV, anti- HIV levels were detected. The results were evaluated by using SPSS (ver. 12) statistics program. Results : 17 male and 23 female patients were taken to study and their average age was 54 +/- 16 year. Their alanineaminotransferase values were 25 +/- 29 IU/L, their aspartat aminotransferase values 20 +/- 12 IU/L, erythrocyte ferritin values 1.8 +/- 1.72 ng/gHb and their serum ferritin values were 992 +/- 424ng/ml. When the data evaluated, it was found that, there is a statistically significant positive correlation between the serum aminotransferase levels and the serum ferritin levels (p<0.05), whereas there was no statistically significant pozitif correlation between aminotransferase levels and erythrocyte ferritin levels (p>0.05). Conclusion: In HCV positive patients, AST, ALT and serum ferritin released from hepatoselluler stores shows liver damage. We found a significant positive correlation between aminotransferase levels and serum ferritin levels, but not with erythrocyte ferritin levels. This finding lead us to a conclusion that, erythrocyte ferritin levels are not a good marker for showing hepatocellular damage as like serum ferritin levels in HCV positive hemodialysis patients.Öğe Evaluation of Some Element and Mineral Levels in Prescription and Non-Prescription Dog Diets(Univ Ljubljana, 2024) Or, Mehmet Erman; Bilgic, Bengu; Tarhan, Duygu; Ates, Fatma; Dokuzeylul, Banu; Gulyasar, TevfikVarious prescription diets prescribed by veterinarians for specific diseases in dogs have been developed and introduced to the market. Trace element and mineral levels, which are essential for healthy living conditions in animals, can differ in both prescription and non-prescription foods. In our study, it was aimed to determine the levels of some elements and minerals in various prescription and non-prescription dry foods used in dog nutrition and to evaluate their therapeutic importance. In the study, a total of 100 different prescription dry food formulated for hepatic diseases (H, n=25), renal diseases (R, n=25), gastrointestinal diseases (GI, n=25) and, allergic diseases (HA, n=25) were used. Non-prescription dry foods from different flavors and brands in the market were considered as the control group (C, n=50). Copper (Cu), Iron (Fe), Manganese (Mn), Zinc (Zn), Selenium (Se), Calcium (Ca), and Phosphorus (P) levels of all dry foods were analyzed by Inductively Coupled Plasma -Optical Emission Spectrometer (ICP-OES, Thermo iCAP 6000 series) and the results were compared between groups. Statistical analysis was evaluated using SPSS 21. Cu levels in GI and HA groups were higher than in the control group (p<0.05 and p<0.01, respectively). Fe levels were higher in the GI group and lower in the HA group than in the control group (p<0.05). Mn level was significantly higher in the H group compared to the control group (p<0.001). The Mn levels in GI and HA groups were higher than the control group (p<0.01). There was no statistical difference in Se and Zn levels between prescription and non-prescription dry foods. Ca and P levels in all groups were statistically lower than in the control group (p<0.001). There are significant differences in element and mineral levels in prescription and nonprescription dry foods. These values may be out of the legal limits determined by EU Regulation. Considering the therapeutic purpose of these prescription formulas, some element and mineral amounts were determined as inappropriate.Öğe EVALUATION OF ZINC AND COPPER TOXICITY CAUSED BY INGESTION OF TURKISH COINS: AN IN VITRO STUDY(Polish Society Magnesium Research, 2020) Alkan, Fatma Ates; Kilinc, Evren; Gulyasar, Tevfik; Or, Mehmet ErmanCoins are foreign objects that are commonly ingested by children and pets due to their shiny appearance and bright colors. The current study investigated whether Turkish coins can lead to zinc (Zn) and copper (Cu) toxicity as a result of exposure to simulated gastric juice (i.e. hydrochloric acid solution simulating the gastric environment). Five groups of coins were exposed to simulated gastric juice (0.15 N, pH:1-2) for a period of 4 (Group 1), 12 (Group 2), 24 (Group 3), 48 (Group 4), 72 (Group 5) and 120 h (Group 5) at body temp. (37 degrees C). Zinc and copper levels were determined in the gastric acid solution by using an inductively coupled plasma optical emission spectrophotometer (ICP-OES). The coins were also evaluated for corrosive damage and weight loss. Group 5 had statistically higher Cu and Zn levels versus the other groups. However, at body temp. (37 degrees C), copper and zinc levels increased steadily in parallel to the duration for which the coin remained in the stomach after ingestion. After 120-hour exposure at 37 degrees C, all coins had various types of damage compared to the baseline, such as color alteration, erosion, and visible surface cavities. The mechanisms relating to local and systemic copper and zinc toxicity caused by coin ingestion is yet to be clarified for both Turkish and international coins. Therefore, it can be foreseen that intervention is required in the first 24 h after ingesting 5 kurus coins and, unless removed spontaneously in 48 h, such intervention is needed for the other coins.Öğe High levels of cadmium and lead in seminal fluid and blood of smoking men are associated with high oxidative stress and damage in infertile subjects(Humana Press Inc, 2007) Kiziler, Ali Riza; Aydemir, Birsen; Onaran, Ilhan; Alici, Bulent; Ozkara, Hamdi; Gulyasar, Tevfik; Akyolcu, Mehmet CanWe measured the levels of malondialdehyde (MDA), protein carbonyls, glutathione S-transferase (GST) and reducte glutathione (GSH) in seminal plasma and spermatozoa from 95 subjects including 50 infertile patients to evaluate the association between oxidative stress and damage and the components of the anti-oxidant defenses in seminal plasma and spermatozoa of infertile subjects and concentrations of cadmium (Cd) and lead (Pb) in the blood and seminal plasma because of tobacco smoke exposure. The reactive oxygen species (ROS) in spermatozoa were also evaluated by luminol (5-amino-2,3-dihydro-1,4-phthalazinedione)-enhanced chemiluminescence assay. The sperm count, motility, and morphology in the smokers infertile group were found to be lower than those in the fertile male group and nonsmokers infertile group (p < 0.001). Concentrations of Cd, Pb, MDA, protein carbonyls, and ROS levels in the smokers infertile group were significantly higher than those in the fertile male and nonsmokers infertile male groups (p < 0.001). However, GSH levels and GST activities were decreased in the smokers infertile male group than those in the fertile male and nonsmokers infertile male groups (p < 0.001). The results indicate that smoking could affect semen quality and oxidative lipid and protein damage in human spermatozoa. From Pearson correlation analysis, positive correlations were demonstrated between the seminal plasma Cd and seminal plasma protein carbonyls and between seminal plasma Pb and spermatozoa ROS levels in smokers of the subfertile group, while there was a significant positive correlation between blood Cd and ROS levels in smokers of the fertile group. There was also a significant negative correlation of the Cd level of the blood and GSH levels of the sperm and seminal plasma. These findings suggest that cigarette smoking enhances the levels of Cd and Pb in seminal plasma and blood and the extent of oxidative damage associated with a decrease in components of the anti-oxidant defenses in the sperm of infertile males.Öğe Investigation of Angiotensinogen M235T and T174M Gene Polymorphisms in Coronary Artery Disease(Erciyes Univ Sch Medicine, 2021) Yamasan, Bilge Eren; Gulyasar, Tevfik; Sipahi, Tammam; Sivri, Nasir; Palabiyik, OrkideObjective: Coronary artery disease (CAD) is a multifactorial disorder and is caused by both environmental and genetic factors. As the alterations in angiotensinogen (AGT) gene lead to changes in angiotensin II and plasma levels of AGT, variants of this gene may play a role in CAD pathogenesis. This study aimed to investigate the relationship between CAD and polymorphisms of AGT gene at M235T and T174M regions. Moreover, the associations of potential risk factors with these gene regions and CAD were investigated. Materials and Methods: In total, the study enrolled 214 cases with CAD and 200 controls. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) were used to detect polymorphisms at M235T and T174M. PCR products were electrophoresed on 2% agarose gel, with ethidium bromide, and were then examined under ultraviolet light. Subsequently, RFLP was used to detect gene polymorphisms. A multiple binary logistic regression model was used to investigate the association of risk factors with both CAD and AGT variants. Results: The number of TT polymorphisms at M235T were significantly higher in the case group than in control group. However, there were no significant differences between cases and controls regarding T174M gene polymorphisms. The presence of hypertension, low high-density lipoprotein level, alcohol consumption, and family history were associated with CAD. Conclusion: TT polymorphisms at the M235T region in AGT can be an influential factor in the development of CAD.Öğe Investigation of Lead Mobilization from the Buckshot Residues to the Critical Organs(Humana Press Inc, 2011) Celbis, Osman; Karakoc, Yunus; Ozdemir, Bora; Gulyasar, Tevfik; Cakina, SuatLead exposure causes neurotoxicity, reproductive system dysfunction, renal failure, and blood and endocrine system disorders in human and experimental animals. In this study, we investigated lead mobilization from gunshot fragments to the critical organs (brain, heart, liver, and kidney) and its interaction with essential trace elements on experimental rat model. Thirty-five rats were randomly divided in five groups. The first group was a control and the others were subjected to buckshot implantation in their skeletal muscles (second and third groups) and abdomen (fourth and fifth groups). The control group and the second and fourth groups were sacrificed 1 month after the onset of experiment while the third and fifth groups were followed after 2 months. Blood lead levels were significantly elevated in both 2 month-followed groups and 1 month-followed intraabdominal group. There were significant increases in brain lead levels of both 2 month-followed groups. For the 1 month-followed groups, kidney lead levels were also significantly higher than those of controls. Results show that lead mobilizes from the buckshot residues in distance tissues to the critical organs and interacts with iron, copper, and zinc even though blood lead level sometimes remains unchanged. Our findings are crucial in revealing lead accumulation in critical organs of subjects carrying any gunshot fragments. These subjects and physicians should be in alert for emergence of lead-induced manifestations.Öğe Investigation of Relationship Between Small Noncoding RNA (sncRNA) Expression Levels and Serum Iron, Copper, and Zinc Levels in Clinical Diagnosed Multiple Sclerosis Patients(Springer, 2023) Ay, Arzu; Alkanli, Nevra; Atli, Engin; Gurkan, Hakan; Gulyasar, Tevfik; Guler, Sibel; Sipahi, TammamIn our study, we aimed to investigate the relationship between microRNA (miRNA) expression levels and serum iron (Fe), copper (Cu), and zinc (Zn) levels in Multiple sclerosis (MS) patients. Total RNA was isolated from peripheral venous blood containing ethylenediaminetetraacetic acid (EDTA) of MS patients and controls. Total RNA was labeled with Cy3-CTP fluorescent dye. Hybridization of samples was performed on microarray slides and arrays were scanned. Data argument and bioinformatics analysis were performed. Atomic absorption spectrophotometer method was used to measure serum Fe, Cu, and Zn levels. In our study, in bioinformatics analysis, although differently expressed miRNAs were not detected between 16 MS patients and 16 controls, hsa-miR-744-5p upregulation was detected between 4 MS patients and 4 controls. This may be stem from the patient group consisting of MS patients who have never had an attack for 1 year. Serum iron levels were detected significantly higher in the 16 MS patients compared to the 16 controls. This may be stem from the increase in iron accumulation based on inflammation in MS disease. According to the findings in our study, hsa-miR-744-5p upregulation has been determined as an early diagnostic biomarker for the development together of insulin resistance, diabetes mellitus associated with insulin signaling, and Alzheimer's diseases. Therefore, hsa-miR-744-5p is recommended as an important biomarker for the development together of diabetes mellitus, Alzheimer's disease, and MS disease. In addition, increased serum Fe levels may be suggested as an important biomarker for neurodegenerative diseases such as Parkinson's disease, Alzheimer's disease, and MS disease.Öğe Investigation of the relationship between GSTM1 gene variations and serum trace elements, plasma malondialdehyde levels in patients with colorectal cancer(Springer, 2021) Ay, Arzu; Gulyasar, Tevfik; Alkanli, Nevra; Sipahi, Tammam; Cicin, Irfan; Kocak, Zafer; Sut, NecdetBackground The aim of this study is to investigate of the relationship between GSTM1 gene variations and serum trace elements, plasma malondialdehyde levels in patient with colorectal cancer. Mateials and Methods. Genotype distributions of GSTM1 gene variations were determined using real-time polymerase chain reaction method. Serum trace element levels were determined using atomic absorption spectrophotometer method and plasma MDA levels were measurement by spectrophotometric method. Results Serum Cu levels, plasma MDA levels and Cu/Zn ratio were determined significantly higher in the group of CRC patient carrying the GA heterozygous genotype of the GSTM1 (rs 112,778,559) gene variation compared to healthy controls (p < 0.05). Serum Cu, Zn levels, plasma MDA levels and Cu/Zn ratio were determined significantly higher in patients carrying GG homozygous genotype of the GSTM1 (rs 112778559) gene variation compared to healthy controls carrying same genotype (p < 0.05). Serum Cu, Zn levels, plasma MDA levels and Cu/Zn ratio were determined significantly higher in the group of CRC patient carrying the GG homozygous genotype of the GSTM1 (rs 12068997) gene variation compared to healthy controls (p < 0.05). On the other hand, serum Se levels were detected significantly lower in CRC patients carrying GA heterozygous and GG homozygous genotypes for GSTM1 (rs 112,778,559) and (rs 12,068,997) gene variations compared to healthy controls (p < 0.05). Conclusion In our study, the evaluation of serum Cu, Zn and Se trace element levels and plasma MDA levels according to GSTM1 gene variations genotype distributions were enabled to obtain important biomarkers in terms of CRC development and progression.Öğe Investigation of the relationship between MTHFR, IRS and CALCA gene polymorphisms and development of diabetic nephropathy in patients with type 2 diabetes mellitus(Taylor & Francis Ltd, 2018) Ay, Arzu; Alkanli, Nevra; Sipahi, Tammam; Gulyasar, Tevfik; Ustundag, Sedat; Guldiken, Sibel; Sut, NecdetThe aim of this study was to investigate the relationship between MTHFR, IRS and CALCA gene polymorphisms and development of diabetic nephropathy (DN) in patients with type 2 diabetes mellitus (DM). Our study included 93 patients with type 2 DM diagnosed as having nephropathy and 95 controls diagnosed with type 2 DM without development of DN. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) were used to determine the genotype distributions of MTHFR, IRS and CALCA gene polymorphisms. The results showed no statistically significant difference between DN patients and type 2 DM controls in terms of genotype distributions of MTHFR (C677T, A1298C), IRS (IRS-1 Gly972Arg, IRS-2 Gly1057Asp) and CALCA T692C gene polymorphisms (p > 0.05). However, in terms of allele frequencies for the MTHFR A1298C gene, the frequency of the C allele was significantly higher in the DN patients compared to the controls (p < 0.05). In the IRS-2 Gly1057Asp gene polymorphism, the G allele frequency was significantly higher in the DN patients than in the type 2 DM controls (p < 0.05). In the DN group, the individuals with one or less mutant alleles were significantly more than in the control group in terms of the IRS-2 Gly1057Asp gene polymorphism (p < 0.05). The C allele frequency for the MTHFR A1298C gene polymorphism and the G allele frequency for the IRS-2 Gly1057Asp gene polymorphism were indicated to be potential a genetic risk factor for the development of DN in patients with type 2 DM who developed DN.Öğe Relationship between cyclin D1 (A870G) gene polymorphism and lung cancer(Natl Inst Science Communication-Niscair, 2013) Cakina, Suat; Gulyasar, Tevfik; Ozen, Alaattin; Sipahi, Tammam; Kocak, Zafer; Sener, SeralpThe roles of many genes in the pathophysiology of lung cancer have been investigated in different studies. Cyclin D1 (CCND1) gene plays a significant role in the transition from G1 to S phase of the cell cycle and in the phosphorylation of retinoblastoma tumor suppressor protein. In this study, we aimed to identify the relationship between CCND1 A870G gene polymorphism with lung cancer. CCND1 A870G genotypes were determined in 75 patients with lung cancer and in 65 control subjects. DNA was isolated from blood samples and then CCND1 A870G gene polymorphism was identified using PCR and RFLP assay. The distribution of CCND1 A870G polymorphism did not show any significant differences in all lung cancer patients and controls. There was no correlation between CCND1 A870G polymorphism and histopathological findings. However, the AA + AG genotype was significantly higher in metastatic patients, when compared with non-metastatic patients. Thus, the results show that CCND1 gene polymorphism may be a predictor for detecting patients with poor survival who having metastatic disease.Öğe Relationships among iron, protein oxidation and lipid peroxidation levels in rats with alcohol-induced acute pancreatitis(Humana Press Inc, 2008) Kiziler, Ali Riza; Aydemir, Birsen; Gulyasar, Tevfik; Unal, Ethem; Gunes, PembegulIt has been previously shown that alcohol induces the damage of pancreatic parenchyma tissue, but the mechanism of this damage is still poorly understood. Assuming that oxygen radical damage may be the involved, we measured markers of oxidative damage in pancreatic tissue, blood serum, plasma, and whole blood of rats with early-stage alcohol-induced acute pancreatitis. Thirty-eight male Wistar rats were divided into three groups: the control group (group 1), the acute pancreatitis group 1 day (group 2), and 3 days (group 3) after the injection of ethyl alcohol into the common biliary duct, respectively. The levels of Fe in tissue and serum, whole blood viscosity, plasma viscosity, fibrinogen and homocysteine (Hcy) levels, erythrocyte and plasma malondialdehyde (MDA), and tissue and plasma protein carbonyl levels were found to be significantly higher in groups 2 and 3 than in group 1. However, the levels of reduced glutathione (GSH) in tissue and erythrocytes were significantly lower in groups 2 and 3 than in group 1. These results suggest that elevated Fe levels in serum and pancreatic tissue in rats with early-stage alcohol-induced acute pancreatitis is associated with various hemorheological changes and with oxidative damage of the pancreas.Öğe Roles of selenoprotein K and selenium status in the etiopathogenesis of preeclampsia: Their relationships with maternal lipid peroxidation, oxidative DNA damage, and glutathione peroxidase(Dustri-Verlag Dr Karl Feistle, 2018) Cinemre, Fatma Behice Serinkan; Aydemir, Birsen; Gulyasar, Tevfik; Cinemre, Hakan; Tuten, Abdullah; Yolmaz, Nevin; Yoldoz, MustafaObjective: Preeclampsia (PE) is a complex disease and the underlying mechanisms are not known yet. It is well known that selenium and oxidative stress play a role in the pathogenesis of various diseases as well as PE. The aim of the study was to investigate the changes in selenium status, as an essential trace element, and selenoprotein K (SelK), a newly described selenoprotein which has been suggested to have an antioxidant function in some tissues in patients with preeclampsia. We also aimed at investigating their relationships with maternal lipid peroxidation, oxidative DNA damage, and glutathione peroxidase (GPx) activities. Materials and methods: 38 healthy pregnant and 48 pregnant women with PE were included in the study. Serum selenium levels were measured by graphite-furnace atomic absorption spectrophotometry. Plasma TBARS levels were analyzed by spectrophotometric method; GPx activities, 8OHdG, and SelK levels were determined by ELISA. Results: Plasma TBARS and 8OHdG levels were found to be increased in the PE group compared to the healthy pregnant group. Plasma SelK levels and GPx activities were found to be lower in the PE group than in the healthy control group. However, there was no significant difference in serum selenium levels between two groups. Furthermore, a significant correlation was determined between plasma SelK levels and GPx activities in PE patients group (r = 0.743; p < 0.01). Likewise, a significant positive correlation was found between plasma 8OHdG and TBARS levels (r = 0.457; p < 0.01), serum selenium levels and plasma GPx activities (r = 0.663; p < 0.01), serum selenium and plasma SelK levels (r = 0.851; p < 0.01) in PE. Conclusion: Our findings indicated that a selenoprotein SelK and a selenoenzyme GPx play a role in the etiopathogenesis of PE. Decreases in these antioxidant systems were associated with increase in lipid and DNA oxidation in PE patients. Underlying mechanisms of SelK and its interactions with selenium status and GPx in PE should be clarified in details with further studies.Öğe THYROID DYSFUNCTIONS DUE TO LITHIUM TREATMENT IN BIPOLAR DISORDER: CHANGES IN OXIDATIVE STRESS, TRACE ELEMENTS, AND HEMORHEOLOGICAL PARAMETERS(Polish Society Magnesium Research, 2020) Bahtiyar, Nurten; Cinemre, Fatma Behice Serinkan; Cinemre, Hakan; Kiziler, Ali Riza; Atagun, Murat Ilhan; Gulyasar, Tevfik; Aydemir, BirsenLithium is one of the most widely used medications for the treatment of bipolar disorder (BD). It also has some side effects on thyroid functions. We aimed to investigate the role of oxidative stress, trace elements, and hemorheological parameters on the pathophysiology of thyroid dysfunctions developed by lithium treatment in patients with BD. Patients with BD were divided into three groups: patients that non-lithium-treated, lithium-treated patients for 4-6 weeks, and lithium-treated patients for 40-68 weeks. Blood samples for analysis were taken before and after the treatment period. After analysis, patients were divided into six groups: non-treatment BD group (Group 1); short-term lithium-treatment group that did not develop thyroid dysfunctions (Group 2); short-term lithium-treatment group that developed hyperthyroidism (Group 3); long-term lithium treatment group that developed hypothyroidism (Group 4), long-term lithium-treatment group that developed hyperthyroidism (Group 5), and long-term lithium-treatment group that did not develop thyroid dysfunctions (Group 6). Plasma and whole blood viscosity levels were significantly increased in Groups 4 and 6 compared to Groups 1, 2, and 3. Hemoglobin levels were lower in Group 4 than in Groups 1, 2, and 5. Fibrinogen values were higher in Groups 4 and 5 than Group 1. Plasma and erythrocyte malondialdehyde levels were higher in Group 4 than In Groups 1, 2, 3, and 5. Also, they were increased in Group 6 in comparison with Groups 2 and 3. Erythrocyte glutathione levels were lower in Groups 4 and 6 than Groups 1, 2, 3 and 5. Plasma protein carbonyls levels were higher in Group 4 than in Group 1, or in Group 5 than in Groups 1, 2, and 3, as well as in Group 6 than Groups 1, and 2. Serum zinc levels were higher in Groups 2, 3 and 6 than in Group1. Serum copper levels increased in Groups 2, 4 and 6 in comparison with Group1. The results of this study indicate that oxidative stress increased with treatment time in lithium-induced thyroid dysfunctions. Also, whole blood viscosity, plasma viscosity, fibrinogen, zinc, and copper levels were affected by lithium treatment and treatment duration induced thyroid dysfunctions.Öğe Trace Elements in a Rat Model of Cadmium Toxicity: the Effects of Taurine, Melatonin and N-Acetylcysteine(Aves Yayincilik, Ibrahim Kara, 2010) Gulyasar, Tevfik; Aydogdu, Nurettin; Cakina, Suat; Sipahi, Tammam; Kaymak, Kadir; Sener, SeralpObjectives: This study was undertaken to investigate copper, zinc, iron, and selenium in a rat model of cadmium toxicity and effects of antioxidant substances such as taurine, melatonin and N-acetylcysteine. Materials and Methods: Ninety male Sprague Dawley rats were divided into nine groups. Group 1 received tap water comprising the controls; the remaining eight groups received 200 mu g/ml cadmium chloride (CdCl2) for three months. Group 2 had CdCl2. Groups 3, 4, and 5 were administered taurine, melatonin and N-acetylcystein for three months together with CdCl2. Groups 6, 7, 8, and 9 had CdCl2 for three months and then only water as the second control or antioxidants for seven days. Cadmium, copper, zinc, iron, and selenium levels of heart and brain were measured by atomic absorption spectrophotometer. Results: Cadmium accumulated in significant amounts in brain and heart tissues when compared with controls. CdCl2 levels in Group 1 and Group 2 were 2.56+/-0.77 and 27.2+/-5.82 in the heart, 46.16+/-14.81 and 300.34+/-58.19 in the brain, respectively (p<0.001). We found that melatonin was more effective in brain tissue (p<0.05) whereas N-acetylcysteine was more effective in heart tissue (p<0.001) against cadmium accumulation. Conclusion: We suggest that taurine, melatonin and N-acetylcysteine have some protective effects in brain and heart tissues against cadmium accumulation. Furthermore, trace element levels were restorated in different degrees after taurine, melatonin and N-acetylcysteine administration.
