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Öğe BIOLOGICAL ACTIVITY EVALUATION OF THREE Hypericum L. SPECIES(Trakya Univ Balkan Yerlesesi Enstituler Binasi, 2022) Eruygur, Nuraniye; Ozpinar, Necati; Ozpinar, Hulya; Atas, Mehmet; Tekin, Mehmet; Cevik, OzgeThis study was performed in order to evaluate in vitro antioxidant, anti-Trichomonas vaginalis, antimicrobial and cytotoxic activities of methanol extracts of aerial parts of Hypericum perforatum L., H. thymbrifolium Boiss. & Noe and H. thymopsis Boiss. The antioxidant activity of methanol extracts was tested with 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2 '-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging and iron chelating assays. The total phenol and flavonoid contents were also determined spectrophotometrically. Cytotoxicity and apoptosis were evaluated on MCF-7 breast cancer and PC3 prostate cancer cell line. Antimicrobial activities were evaluated by the microdilution method on the bacterial strains Staphylococcus aureus (ATCC 29213), Enterococcus faecalis (ATCC 29212), Pseudomonas aeruginosa (ATCC 27853) Escherichia coli (ATCC 25922) and the fungal strain Candida albicans (ATCC 10231). All extracts demonstrated cytotoxic activity on PC3 prostat cancer cell line with a concentration dependent manner, while H. thymopsis extract was the most active against cancer cell growth. The obtained results of the present study revealed that the methanol extract of H. perforatum, H. thymbrifolium and H. thymopsis have significant antioxidant and cytotoxic activities. The results provided the basic research data for further phytochemical and biological activity guided investigations on these species for identication and isolation of potential drug active compounds.Öğe Evaluation of Anticholinergic, Antidiabetic and Antioxidant Activity of Astragalus dumanii, an Endemic Plant(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2022) Kocyigit, Umit Muhammet; Eruygur, Nuraniye; Atas, Mehmet; Tekin, Mehmet; Taslimi, Parham; Gokalp, Faik; Gulcin, IlhamiThe research was conducted to separately evaluate and detect the possible in vitro antioxidant, antimicrobial activity of ethanol extracts prepared from aerial parts and roots of Astragalus dumanii and anti-cholinesterase and alpha-glucosidase inhibitory activity from only aerial parts of its The antioxidant capacity was tested by scavenging of DPPH and ABTS free radicals. Compared with the standard antioxidant compound gallic acid; Root and aerial part extract showed lower DPPH radical scavenging activity, however aerial part extract demonstrated higher ABTS radical scavenging activity. The phenolic contents were detected as 5.31 +/- 0.03 and 13.23 +/- 0.05 mg gallic acid equivalent g-1 extract, flavonoid contents were found as 8.26 +/- 0.004 and 7.93 +/- 0.005 mg Qercetin equivalent g-1 extract. In addition, the effects of the extracts obtained from aerial parts of the plant on acetylcholinesterase, butyrylcholinesterase and a-glycosidase enzymes were investigated in vitro and IC50 values were obtained as 1.47, 0.83 and 0.48 mu g mL-1, respectively. When these values were compared with standard substances, it was seen that Astragalus dumanii could be a good enzyme inhibitory agent. Antimicrobial activity of the plant extracts were determined using the microdilution method and the extracts was not observed to have any antimicrobial activities..Öğe Evaluation of in vitro Antioxidant, Antimicrobial and Cytotoxic Activities of Crude Ethanol Extract and Fractions of Achillea sintenisii Hub. Mor.(Marmara Univ, Inst Health Sciences, 2023) Eruygur, Nuraniye; Atas, Mehmet; Tekin, Mehmet; Cevik, OzgeObjective: The Achillea species have been used to treat various ailments due to its anti-inflammatory, hemostatic, spasmolytic and cholagogue effects in the Turkish traditional medicine. However, there is no biological activity studies on some Achillea species except for the well-knowns. This work aimed to determine the antioxidant, antimicrobial and cytotoxic activities of the crude ethanolic extracts and fractions of Achillea sintenisii using in vitro methods. Methods: The antioxidant activity was investigated by DPPH (1,1'-diphenyl - 2-picrylhydrazyl), ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging, total phenol and total flavonoid content, and iron chelating methods. Antimicrobial activity evaluated by micro-plate dilution method against five test organisms. Cytotoxicity was determined by MTT method using MCF-7 breast cancer cell line and PC-3 prostate cancer cell line. Apoptosis was also measured by AO/EB staining. Results: The n-Hexane fractions showed the highest antimicrobial and cytotoxic activities, respectively. Administration of the extracts on the cancer cells showed a concentration dependent inhibition on cell proliferation. The anti-proliferation effect could be via apoptosis and associated with the cell death. Conclusion: The results showed that the extracts demonstrated antioxidant, antimicrobial, and cytotoxic activity, also supports the claims of traditional usage.Öğe In Vitro Antioxidant and Enzyme Inhibition Activity of Tanacetum argyrophyllum (K. Koch) Tzvelev var. argyrophyllum Extract(Galenos Publ House, 2022) Eruygur, Nuraniye; Akca, Keyser Taban; Ustun, Osman; Tekin, MehmetObjectives: Tanacetum L. belongs to Asteraceae family and is represented by 46 species in Turkiye. Tanacetum genus is known for its insecticide and insect repellent effect. T. argyrophyllum contains sesquiterpene lactone derivatives. These compounds are responsible for its various activities, especially cytotoxic, antitumor, phytotoxic, antimicrobial, antiviral, and antifungal activity. There are not enough biological activity studies on the plant that are likely to have a wide variety of activities in terms of the compounds it contains. The aim of the present study is to evaluate various biological activities of 80% aqueous methanol extract prepared from aerial parts of T. argyrophyllum (K. Koch) Tzvelev var. argyrophyllum collected from Sivas province of Turkey. Materials and Methods: Antioxidant activity of the methanol extract was determined by 2,2'-diphenyl-1-picryl-hydrazyl and 2,2-azinobis (3-ethyl benzothiazoline-6-sulfonic acid) radical scavenging activity, total phenolic, and total flavonoid content tests. Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activities were investigated via Ellmans spectrometric method. Results: Total phenolic content was found as 71.67 mg/gallic acid equivalent g and total flavonoid content was 25.225 mg/quercetin equivalent g on dry extract weight basis. In this work, AChE, BChE, and alpha-glycosidase enzymes were inhibited by the extract of T. argyrophyllum var. argyrophyllum. IC50 values for these enzymes were found as 266.79 mu g/mL for AChE and 176.91 mu g/mL for BChE. Also, the alpha-glycosidase activity exhibited a dose-dependent manner with increasing concentration. Conclusion: According to the results, T. argyrophyllum var. argyrophyllum can be used as an ingredient of functional foods as well as herbal products for diabetic and Alzheimer's disease patients.Öğe IN-VITRO ANTIOXIDANT, A-GLUCOSIDASE AND A-AMYLASE INHIBITORY ACTIVITIES OF CRUDE ETHANOL EXTRACT AND FRACTIONS OF ENDEMIC HYACINTHELLA ACUTILOBA K.PERSS. & WENDELBO BULBUS(Parlar Scientific Publications (P S P), 2019) Eruygur, Nuraniye; Dural, Emrah; Tekin, Mehmet; Ozpinar, HulyaIn the present study, the antioxidant potential as well as the alpha-amylase and alpha-glucosidase inhibitory activity of different extracts of Hyacinthella acutiloba bulbus were investigated using an in vitro model. Antioxidant activity was assayed by the DPPH radical scavenging activity, total phenol content (TPC) and total flavonoid content (TFC), reducing power assay, ferric thiocyanate (FTC) and thiobarbituric acid method (TBA). Among the extracts, n-hexane and chloroform extract of H. acutiloba displayed more activity than other extracts against a amylase and alpha-glucosidase.Öğe Investigating of Phytochemicals, Antioxidant, Antimicrobial and Proliferative Properties of Different Extracts of Thymus spathulifolius Hausskn. and Velen. Endemic Medicinal Plant from Sivas, Turkey(2017) Eruygur, Nuraniye; Ataş, Mehmet; Çevik, Özge; Tekin, MehmetThymus species has been used for antioxidant, antiseptic, antitussive, carminative, anti-inflammatory and antimicrobial activities as well as tonic and herbal teas. The present study was conducted to evaluate antioxidant, antimicrobial and proliferative properties of ethanol crude extract and fractions of Thymus spathulifolius (Hausskn. and Velen.) herbes. The antioxidant properties of ethanol extracts and fractions of Thymus spathulifolius were investigated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, reducing power, ferrous chelating activity, total flavonoid and total phenolic content analysis. Antimicrobial activity of the plant extracts were tested using the microdilution method, while proliferative activity were evaluated by MTT assay. Results showed that IC50 of T. spathulifolius extracts that scavenged 50% of the DPPH radical was found to be ranged from 62.39 to 1000 µg/mL. Hexane extract possessed moderate antimicrobial activity towards grampositive bacteria of S. aureus and fungi of C. albicans. S. aureus was the most sensitive bacteria than other tested microorganisms. The hexane and water extracts exhibited a good proliferative activity with ED50 of 3.28 µg/mL and 2.77 µg/mL, respectively. The antioxidant and antimicrobial activities, together with the ability of proliferation, provide some support for the T.spathulifolius's traditional use.Öğe Pharmacognostic, Physicochemical, Phytochemical Screening and In-Vitro Antioxidant Activity of Chrysochamela Noeana, Endemic in Türkiye(2022) Eruygur, Nuraniye; Tekin, Mehmet; Dönmez, ErolThe aim of the present study was to conduct preliminary phytochemical screening, pharmacognostical and physicochemical investigation of Chrysochamela noeana. The fresh and dried herbs were studied by morphology, microscopy, preliminary phytochemical screening and florescence analysis of powdered drug. In addition, physicochemical parameters were studied according to WHO guidelines. Physicochemical parameters and florescence analysis were also performed. The preliminary phytochemical analysis revealed the presence of steroids, flavonoids, phenolic compounds, and carbohydrates. Total phenol contents were 245 ± 3.67, 179.06 ± 4.52, and 15.98 ± 3.32 (GAE mg/g extract) and total flavonoid content were 180.85 ± 8.21, 146.41 ± 2.56, and 13.46 ± 0.23 (QUE mg/g extract) for ethyl acetate, methanol, and water extracts, respectively. The IC50 value for extracts in ABTS radical scavenging activity were calculated in order of 51.47, 122.26 and 1146.91 ?g/mL, and in DPPH for 1.55, 1.13 and 27.16 mg/mL for ethyl acetate, methanol, and water extracts, respectively. The results of these studies could be useful for correct identification and detection of adulterants from this plant material.Öğe Phytochemical Profiling and Biological Activity of Achillea sintenisii Hub.-Mor(Wiley-V C H Verlag Gmbh, 2023) Eruygur, Nuraniye; Buyukyildirim, Tugsen; Tetik Rama, Seyma; Ayaz, Fatma; Tekin, Mehmet; Tuzcu, Mehmet; Akcakavak, GokhanAchillea (Asteraceae) species have been traditionally used for their different therapeutical properties. In this study, phytochemical composition of aerial parts of A. sintenisii which is endemic in Turkey was determined with Liquid chromatography/mass spectrometry/mass spectrometry (LC/MS/MS). To evaluate the wound healing potential, the cream formulation prepared from A. sintenisii was tested on the linear incision wound model in mice. In vitro enzyme inhibitory activity tests were performed on elastase, hyaluronidase, and collagenase. In the histopathological examination, angiogenesis and granulation tissue formation were significantly increased in A. sintenisii treatment groups compared to the negative control group. As a result of this study, it is thought that the enzyme inhibition and antioxidant activity of the plant may contribute to the wound healing process. According to LC/MS/MS analysis result, quinic acid (24.261 mu g/mg extract) and chlorogenic acid (14.97 mu g/mg extract) were identified as main constituents of the extract.Öğe A Preliminary Phytochemical, Pharmacognostic and Physicochemical Evaluation of Endemic Thymus spathulifolius (Lamiaceae)(Kastamonu Univ, 2023) Eruygur, Nuraniye; Akyol, Yurdanur; Tekin, MehmetAim of study: The members of the genus Thymus are widely used in Turkey as herbal teas, spices and salads for different purpose and the herbs of Thymus spathulifolius are reported to have medicinal value. Therefore, in this study various pharmacognostic parameters, physicochemical properties and anatomical characters of Thymus spathulifolius Hausskn. & Velen., is a medicinal plant and endemic to Turkey, were investigated.Area of study: T. spathulifolius was dried and stored according to herbarium techniques in the Herbarium of Pharmacy Faculty, Trakya University, collected from Sivas, Turkey.Material and methods: The total ash, moisture content, and physicochemical tests on the different extracts obtained from the endemic T. spathulifolius aerial parts collected from Sivas province were determined according to the World Health Organization (WHO) standards for crude drugs.Main results: The moisture content and total ash amount of the drug obtained from this plant is in accordance with the WHO standards. Phytochemical screening for secondary metabolites showed the presence of carbohydrates, antraquinone glycosides, steroids, flavonoids and coumarins, while other phytochemicals such as alkaloids and saponins were absent. Besides, the cross-sections of root, stem and leaf were examined and detailed. The total ash value was found as 7.36 +/- 0.21% and acid insoluble ash was 0.14 +/- 0.72%. Highlights: The present study provided important information for correct authentication and standardization of this plant material.
