Development of a reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of Amasya cherry disease

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dc.authoridKozlakidis, Zisis/0000-0002-3836-7209
dc.authorwosidKozlakidis, Zisis/AAX-8081-2020
dc.contributor.authorKozlakidis, Z.
dc.contributor.authorCitir, A.
dc.contributor.authorAcikgoz, S.
dc.contributor.authorCoutts, R. H. A.
dc.date.accessioned2024-06-12T10:51:22Z
dc.date.available2024-06-12T10:51:22Z
dc.date.issued2007
dc.departmentTrakya Üniversitesien_US
dc.description.abstractA reverse transcription-polymerase chain reaction (RT-PCR) procedure was developed to detect Amasya cherry disease (ACD) in naturally infected sweet cherry (Prunus avium) leaves sampled from Turkey. The procedure was based on detection of the presence of a mycoviral-like double-stranded RNA (dsRNA) of 5.3 kbp always found in association with ACD, which is probably caused by a fungus. Specific primers were designed to amplify a fragment of the diagnostic dsRNA. The method will improve routine diagnosis of ACD in Prunus spp.en_US
dc.identifier.doi10.1111/j.1365-3059.2007.01690.x
dc.identifier.endpage1035en_US
dc.identifier.issn0032-0862
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-35948953640en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1032en_US
dc.identifier.urihttps://doi.org/10.1111/j.1365-3059.2007.01690.x
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18341
dc.identifier.volume56en_US
dc.identifier.wosWOS:000250763200013en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWiley-Blackwell Publishing, Incen_US
dc.relation.ispartofPlant Pathologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAmasya Cherry Diseaseen_US
dc.subjectCherry Treesen_US
dc.subjectDiagnosisen_US
dc.subjectDsrnasen_US
dc.subjectPrunus Aviumen_US
dc.subjectChlorotic Rusty Spoten_US
dc.subjectDouble-Stranded Rnasen_US
dc.subjectComplex Patternen_US
dc.titleDevelopment of a reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of Amasya cherry diseaseen_US
dc.typeArticleen_US

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