Development of a reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of Amasya cherry disease
Küçük Resim Yok
Tarih
2007
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-Blackwell Publishing, Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A reverse transcription-polymerase chain reaction (RT-PCR) procedure was developed to detect Amasya cherry disease (ACD) in naturally infected sweet cherry (Prunus avium) leaves sampled from Turkey. The procedure was based on detection of the presence of a mycoviral-like double-stranded RNA (dsRNA) of 5.3 kbp always found in association with ACD, which is probably caused by a fungus. Specific primers were designed to amplify a fragment of the diagnostic dsRNA. The method will improve routine diagnosis of ACD in Prunus spp.
Açıklama
Anahtar Kelimeler
Amasya Cherry Disease, Cherry Trees, Diagnosis, Dsrnas, Prunus Avium, Chlorotic Rusty Spot, Double-Stranded Rnas, Complex Pattern
Kaynak
Plant Pathology
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
56
Sayı
6
