Edirne ilindeki kreş ve gündüz bakımevlerinin iç ve dış ortamında havayla taşınan funguslar ve bakteriler
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Dosyalar
Tarih
2006
Yazarlar
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Yayıncı
Trakya Üniversitesi Fen Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, Edirne ilindeki altı yaş öncesi çocukların bulunduğu ve eğitim gördüğü 4 farklı kreş ve gündüz bakımevinin iç ve dış havasındaki bakteri ve mikrofunguslar ile yoğunluklarının ve bunların istasyonlara göre, aylık, mevsimsel ve meteorolojik faktörlerle ilişkisinin araştırılması amaçlanmıştır. Bu amaçla "Yerçekimine Dayalı Petri Plak Metodu" kullanılarak içlerinde bakteriler için %5 Koyun Kanlı Beyin Kalp nfüzyon Agar, mikrofunguslar için Rose-Bengal ve Streptomycin ilaveli Pepton Dekstroz Agar besiyerlerinin bulunduğu petri plaklarının hava ile temas ettirilmesi sağlanmak sureti ile, kreş ve gündüz bakımevlerinin 3 farklı iç ortamından ve 1 dış ortamından, Ocak 2004-Aralık 2004 tarihlerini kapsayan 12 aylık sürede ayda bir defa olmak üzere örnekleme işlemi yapılmıştır. Mikrofungus izolasyonu için toplam 192 petri plağı kullanılmış ve bu plaklarda 907'si iç ortamdan, 1164'ü dış ortamdan olmak üzere toplam 2071 mikrofungus kolonisi sayılmıştır. Bakteri izolasyonu için kullanılan 192 petri plağında da 1406'sı iç ortamdan, 1714'ü dış ortamdan olmak üzere toplam 3120 bakteri kolonisi sayılmıştır. Çalışma sonucunda, 30 mikrofungus cinsine (Acremonium, Alternaria, Arthrinium, Aspergillus, Bahusakala, Beauveria, Ceuthospora, Chaetomium, Cladosporium, Curvularia, Drechslera, Epicoccum, Eurotium, Fusarium, Mycotypha, Myrotechium, Paecilomyces, Penicillium, Pestalotiopsis, Phoma, Ramichloridium, Rhizopus, Scopulariopsis, Stachybotrys, Stemphylium, Torula, Trichoderma, Trictothecium, Ulocladium, Verticillium) ait 75 mikrofungus türü ile Gram(+) kok, Gram(+) basil ve Gram(-) basillere ait bakteriler tespit edilmiştir. Cladosporium, Penicillium, Alternaria en sık izole edilen mikrofungus cinsleri iken, Penicillium 26 türle en fazla tür zenginliğine sahip mikrofungus cinsi olmuştur. Staphylococcus, Bacillus ve Corynebacterium ise en sık izole edilen bakteri cinsleridir. Araştırma süresince her ay Alternaria, Cladosporium, Penicillium cinsleri ve sporsuz mikrofunguslar bulunmuştur. Her ay bulunan bakteri cinsleri ise Staphylococcus, Streptococcus, Bacillus ve Corynebacterium'dur. Araştırma periyodu boyunca izole edilen bakteri ve mikrofungus cinslerinin ve toplam bakteri ve mikrofungus konsantrasyonlarının, çeşitli meteorolojik faktörlerle arasında ilişki olup olmadığını tespit etmek amacıyla istatistiksel analizler yapılmıştır. Yapılan korelasyon analizleri sonucunda dış ortamdaki aylık toplam mikrofungus sayısı ile aylık ortalama sıcaklık arasında pozitif, aylık ortalama rüzgar hızı arasında negatif ilişkiler; dış ortamdaki aylık toplam bakteri sayısı ile günlük ortalama nisbi nem, aylık ve günlük ortalama yağış miktarı arasında da negatif ilişkiler bulunmuştur. En fazla izole edilen Cladosporium, Penicillium, Alternaria mikrofungus cinslerinin ve sporlu Gram(+) basil bakterilerin dış ortam aylık konsantrasyonları ile çeşitli meteorolojik parametreler arasında ilişkiler tespit edilmiştir.
It was aimed to investigate the densities, monthly and seasonal distribution and relation with climatic factors of indoor and outdoor microfungi and bacteria in four day- care centers of Edirne city. Samples were collected one month intervals and over a period of 12 months between January 2004-December 2004, by exposing petri plates which contained Pepton Dextrose Agar with Rose Bengal and Streptomycin for microfungi and Brain Heart Infusion Agar with Sheep Blood 5% for bacteria to air (Petri Plate Gravitational Settling Method). The 192 petri plates were used for microfungi and bacteria isolations and a total of 2071 microfungi (907 of indoor and 1164 of outdoor) and 3120 bacteria colonies (1406 of indoor and 1714 of outdoor) were counted in these plates. As a result of this study, 30 microfungal genera (Acremonium, Alternaria, Arthrinium, Aspergillus, Bahusakala, Beauveria, Ceuthospora, Chaetomium, Cladosporium, Curvularia, Drechslera, Epicoccum, Eurotium, Fusarium, Mycotypha, Myrotechium, Paecilomyces, Penicillium, Pestalotiopsis, Phoma, Ramichloridium, Rhizopus, Scopulariopsis, Stachybotrys, Stemphylium, Torula, Trichoderma, Trictothecium, Ulocladium, Verticillium), 75 microfungal species and Gram(+) coc, Gram(+) bacil and Gram(-) bacil bacteria were isolated from indoor and outdoor air of day-care centers. Most populous microfungi were Cladosporium, Penicillium, Alternaria, while the genus of most species richness was Penicillium. Staphylococcus, Bacillus and Corynebacterium were the most abundant bacterial genera in this study areas. During sampling, Alternaria, Cladosporium, Penicillium and non-sporulating microfungi were isolated every months. However, Staphylococcus, Streptococcus Bacillus and Corynebacterium bacteria were found in all months. Statistical analyses were performed between concentrations of total microfungi and bacteria and concentrations of microfungi and bacteria genera isolated during investigation and some meteorolojical factors. While positive correlation was found between the concentration of monthly outdoor microfungi and average monthly temperature, negative correlation was found between the concentration of monthly outdoor microfungi and average monthly wind velocity and also between the monthly outdoor bacteria concentration and average daily relative humidity, average monthly and daily rainfall parameters. Some correlations were found between the monthly outdoor concentrations of most isolated microfungal genus (Cladosporium, Penicillium, Alternaria) and various meteorological factors.
It was aimed to investigate the densities, monthly and seasonal distribution and relation with climatic factors of indoor and outdoor microfungi and bacteria in four day- care centers of Edirne city. Samples were collected one month intervals and over a period of 12 months between January 2004-December 2004, by exposing petri plates which contained Pepton Dextrose Agar with Rose Bengal and Streptomycin for microfungi and Brain Heart Infusion Agar with Sheep Blood 5% for bacteria to air (Petri Plate Gravitational Settling Method). The 192 petri plates were used for microfungi and bacteria isolations and a total of 2071 microfungi (907 of indoor and 1164 of outdoor) and 3120 bacteria colonies (1406 of indoor and 1714 of outdoor) were counted in these plates. As a result of this study, 30 microfungal genera (Acremonium, Alternaria, Arthrinium, Aspergillus, Bahusakala, Beauveria, Ceuthospora, Chaetomium, Cladosporium, Curvularia, Drechslera, Epicoccum, Eurotium, Fusarium, Mycotypha, Myrotechium, Paecilomyces, Penicillium, Pestalotiopsis, Phoma, Ramichloridium, Rhizopus, Scopulariopsis, Stachybotrys, Stemphylium, Torula, Trichoderma, Trictothecium, Ulocladium, Verticillium), 75 microfungal species and Gram(+) coc, Gram(+) bacil and Gram(-) bacil bacteria were isolated from indoor and outdoor air of day-care centers. Most populous microfungi were Cladosporium, Penicillium, Alternaria, while the genus of most species richness was Penicillium. Staphylococcus, Bacillus and Corynebacterium were the most abundant bacterial genera in this study areas. During sampling, Alternaria, Cladosporium, Penicillium and non-sporulating microfungi were isolated every months. However, Staphylococcus, Streptococcus Bacillus and Corynebacterium bacteria were found in all months. Statistical analyses were performed between concentrations of total microfungi and bacteria and concentrations of microfungi and bacteria genera isolated during investigation and some meteorolojical factors. While positive correlation was found between the concentration of monthly outdoor microfungi and average monthly temperature, negative correlation was found between the concentration of monthly outdoor microfungi and average monthly wind velocity and also between the monthly outdoor bacteria concentration and average daily relative humidity, average monthly and daily rainfall parameters. Some correlations were found between the monthly outdoor concentrations of most isolated microfungal genus (Cladosporium, Penicillium, Alternaria) and various meteorological factors.
Açıklama
Doktora Tezi
Anahtar Kelimeler
Mikrofungus, Bakteri, İç Ortam Havası, Dış Ortam Havası, Kreş, Microfungi, Bacteria, Indoor Air, Outdoor Air, Day-Care Center
