Homocysteine-BSA - affinity based biosensor design

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dc.authorwosidAyhan, F./AAV-6569-2021
dc.contributor.authorAyhan, Fatma
dc.contributor.authorKaya, Gizem
dc.contributor.authorAyhan, Hakan
dc.date.accessioned2024-06-12T11:12:24Z
dc.date.available2024-06-12T11:12:24Z
dc.date.issued2014
dc.departmentTrakya Üniversitesien_US
dc.description.abstractObjective: In this research, the detection and Reusability of Homocysteine by piezoelectric based Homocysteine Bioaffinity sensor using Quartz Crystal Microbalance (QCM) was aimed. Methods: The surface of silver sensor was subjected to a series of pretreatments. Surface activation process was done with the interaction of silver crystals with NaOH, acetone and methanol, respectively at definite times. Then, systeamine, glutaraldehyde attachments and BSA immobilization were performed to surfaces. BSA concentration was assayed within a range and the highest coupling BSA amount of 0.1 mg/mL was chosen as the most convenient concentration. Results: In the scope of BSA-Homocysteine affinity, the calibration graph of Homocysteine was formed after surface modification steps and the range of 0.5-2 mu mol was estimated to give the best determination coefficient. Reusability of quartz crystals were tested by the cleavage of BSA-Homocysteine affinity in acidic and basic conditions. The NaOH solutions of 10-6, 10-3, 10-1, 1, 10 ve 100 mM (pH 8-9) were used to cleave the affinity and it was observed that no basic medium trials were convenient to reuse. Assays performed to break BSA-Homocysteine affinity in acidic condition were done with 0.01, 1, 10, 100 mM Glisin-HCl solutions of pH 2.5, 3.5, 5.5 and 6.5. The crystal reusability of 12 was reached with Glisin-HCl solution of 0.01, mM in pH 5.5. Conclusion: The highest reusability value of 30 repeat was reached when Glisin-HCl concentration is 1mM with pH 5.5 achieved to cleave BSA-Homocysteine affinity experiments. The experiments carried out in 10 mM Glisin-HCl acidic medium concentration gave oppurtunity to four reuse of silver crystal.en_US
dc.identifier.doi10.5505/tjb.2014.94834
dc.identifier.endpage396en_US
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.issue3en_US
dc.identifier.startpage383en_US
dc.identifier.urihttps://doi.org/10.5505/tjb.2014.94834
dc.identifier.urihttps://hdl.handle.net/20.500.14551/23163
dc.identifier.volume39en_US
dc.identifier.wosWOS:000346148200019en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isotren_US
dc.publisherWalter De Gruyter Gmbhen_US
dc.relation.ispartofTurkish Journal Of Biochemistry-Turk Biyokimya Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPiezoelectric Sensorsen_US
dc.subjectBiosensoren_US
dc.subjectBioaffinityen_US
dc.subjectHomocysteineen_US
dc.subjectBovine Serum Albumineen_US
dc.subjectQuartz Crystal Microbalance (QCM)en_US
dc.subjectNanobiosensoren_US
dc.subjectReusabilityen_US
dc.subjectPiezoelectric Quartz-Crystalen_US
dc.subjectPlasma Homocysteineen_US
dc.subjectRisk-Factoren_US
dc.subjectMicrobalanceen_US
dc.subjectDeterminantsen_US
dc.subjectProteinen_US
dc.subjectDrugsen_US
dc.titleHomocysteine-BSA - affinity based biosensor designen_US
dc.typeArticleen_US

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