Homocysteine-BSA - affinity based biosensor design
Küçük Resim Yok
Tarih
2014
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Walter De Gruyter Gmbh
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Objective: In this research, the detection and Reusability of Homocysteine by piezoelectric based Homocysteine Bioaffinity sensor using Quartz Crystal Microbalance (QCM) was aimed. Methods: The surface of silver sensor was subjected to a series of pretreatments. Surface activation process was done with the interaction of silver crystals with NaOH, acetone and methanol, respectively at definite times. Then, systeamine, glutaraldehyde attachments and BSA immobilization were performed to surfaces. BSA concentration was assayed within a range and the highest coupling BSA amount of 0.1 mg/mL was chosen as the most convenient concentration. Results: In the scope of BSA-Homocysteine affinity, the calibration graph of Homocysteine was formed after surface modification steps and the range of 0.5-2 mu mol was estimated to give the best determination coefficient. Reusability of quartz crystals were tested by the cleavage of BSA-Homocysteine affinity in acidic and basic conditions. The NaOH solutions of 10-6, 10-3, 10-1, 1, 10 ve 100 mM (pH 8-9) were used to cleave the affinity and it was observed that no basic medium trials were convenient to reuse. Assays performed to break BSA-Homocysteine affinity in acidic condition were done with 0.01, 1, 10, 100 mM Glisin-HCl solutions of pH 2.5, 3.5, 5.5 and 6.5. The crystal reusability of 12 was reached with Glisin-HCl solution of 0.01, mM in pH 5.5. Conclusion: The highest reusability value of 30 repeat was reached when Glisin-HCl concentration is 1mM with pH 5.5 achieved to cleave BSA-Homocysteine affinity experiments. The experiments carried out in 10 mM Glisin-HCl acidic medium concentration gave oppurtunity to four reuse of silver crystal.
Açıklama
Anahtar Kelimeler
Piezoelectric Sensors, Biosensor, Bioaffinity, Homocysteine, Bovine Serum Albumine, Quartz Crystal Microbalance (QCM), Nanobiosensor, Reusability, Piezoelectric Quartz-Crystal, Plasma Homocysteine, Risk-Factor, Microbalance, Determinants, Protein, Drugs
Kaynak
Turkish Journal Of Biochemistry-Turk Biyokimya Dergisi
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
39
Sayı
3
