Polyvinyl alcohol-trypsin as a catalyst for amino acid ester synthesis in organic media
Küçük Resim Yok
Tarih
2004
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Marcel Dekker Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The bovine trypsin-catalyzed synthesis of N-alpha-benzoyi-DL-arginine esters from N-benzoyl-DL-arginine were studied in various organic solvents. Trypsin was immobilized to polyvinyl alcohol (PVA) by adsorption from its aqueous solutions. Immobilized enzyme showed higher catalytic activities than free enzyme for amino acid esterification in ethanol. The yield of ester is strongly dependent upon the PVA/trypsin ratio and water content in the reaction medium. The rate and equilibrium constant of the ester formation reaction are also dependent on water content.
Açıklama
Anahtar Kelimeler
Enzymatic Reaction, Ester Synthesis, Bovine Trypsin, Organic Solvent, Water Activity, Peptide-Synthesis, Dipeptide Synthesis, Stabilized Trypsin, Water Activity, Lipase, Protease
Kaynak
Preparative Biochemistry & Biotechnology
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
34
Sayı
4
