Pectolytic enzymes produced by Alternaria alternata in culture and during colonization of common wheat kernels

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dc.authorscopusid7006798815
dc.contributor.authorÖzer N.
dc.date.accessioned2024-06-12T10:27:56Z
dc.date.available2024-06-12T10:27:56Z
dc.date.issued2009
dc.description.abstractThe culture filtrates of seven Alternaria alternata isolates obtained from the black pointed kernels of common wheat (Triticum aestivum) were assayed for pectin lyase (PNL) and polygalacturonase (PG) activity. After isoelectric focusing the patterns of their isoenzymes were defined. PNL and PG activity were detected in all isolates and the differences were also found in the activities of the isolates. Two faint PNL bands were observed in three isolates. All isolates produced PG isoenzyme patterns with bands differing in intensity and in pI values. Healthy wheat kernels were collected at the stages of anthesis, complete flowering, milk, mid-dough and ripe separately, and they were inoculated with an isolate being able to high enzyme activity. Infected kernels were sampled for the black point incidence and assayed for PNL and PG activity. No PNL activity and isoenzymes were found in inoculated kernels in any stage. However the isolates of A. alternata differed in virulence and in quantitative and qualitative PG production in the kernels at different growth stages. The results suggest that particular PGs from A. alternata may contribute to virulence during kernel colonization.en_US
dc.identifier.endpage571en_US
dc.identifier.issn0026-3648
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-77749285669en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage564en_US
dc.identifier.urihttps://hdl.handle.net/20.500.14551/16997
dc.identifier.volume43en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.relation.ispartofMikologiya I Fitopatologiyaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlternaria Alternata; Pectolytic Enzymes; Triticum Aestivumen_US
dc.subjectAlternaria Alternata; Triticum Aestivumen_US
dc.titlePectolytic enzymes produced by Alternaria alternata in culture and during colonization of common wheat kernelsen_US
dc.typeArticleen_US

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