Pectolytic enzymes produced by Alternaria alternata in culture and during colonization of common wheat kernels
Küçük Resim Yok
Tarih
2009
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The culture filtrates of seven Alternaria alternata isolates obtained from the black pointed kernels of common wheat (Triticum aestivum) were assayed for pectin lyase (PNL) and polygalacturonase (PG) activity. After isoelectric focusing the patterns of their isoenzymes were defined. PNL and PG activity were detected in all isolates and the differences were also found in the activities of the isolates. Two faint PNL bands were observed in three isolates. All isolates produced PG isoenzyme patterns with bands differing in intensity and in pI values. Healthy wheat kernels were collected at the stages of anthesis, complete flowering, milk, mid-dough and ripe separately, and they were inoculated with an isolate being able to high enzyme activity. Infected kernels were sampled for the black point incidence and assayed for PNL and PG activity. No PNL activity and isoenzymes were found in inoculated kernels in any stage. However the isolates of A. alternata differed in virulence and in quantitative and qualitative PG production in the kernels at different growth stages. The results suggest that particular PGs from A. alternata may contribute to virulence during kernel colonization.
Açıklama
Anahtar Kelimeler
Alternaria Alternata; Pectolytic Enzymes; Triticum Aestivum, Alternaria Alternata; Triticum Aestivum
Kaynak
Mikologiya I Fitopatologiya
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
43
Sayı
6
