Immunohistochemical demonstration of cytochrome oxidase in different parts of the central nervous system: A comparative experimental study
Küçük Resim Yok
Tarih
2000
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Blackwell Wissenschafts-Verlag Gmbh
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Cytochrome oxidase, the terminal enzyme of the electron transport chain, is a marker of the functional activity of the cell. In this study; localization of cytochrome oxidase in cerebrum, cerebellum, hippocampus, substantia nigra and choroid plexus of adult rats was investigated using immunohistochemical methods. Neural bodies were immunoreactive while neuroglial cells and axonal areas did not show significant immunostaining. The cerebral cortical substantia grisea region was stained almost homogeneously with cytochrome oxidase. In the cerebellar cortex, immunolabelling was more intense in the granular layer than the molecular layer. There was significant immunostaining in Purkinje cells. White matter, both in cerebrum and cerebellum, did not show immunoreactivity for cytochrome oxidase. Neurones in the hippocampus showed variable immunostaining; some of them were negative while others revealed high immunoreactivity. The neurones in substantia nigra were heavily labelled. Immunostaining for cytochrome oxidase in plexus choroideus epithelial cells was also remarkable. The morphological findings demonstrate the regions which most require and produce energy and reflect the differences in cellular activity in these parts of the central nervous system.
Açıklama
Anahtar Kelimeler
Rat-Brain, Histochemistry, Mitochondrial, Expression, Metabolism, Subunits, Neurons, Hippocampus, Invitro, Nuclear
Kaynak
Anatomia Histologia Embryologia-Journal Of Veterinary Medicine Series C
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
29
Sayı
6
