CYTOTOXIC AND ANTIOXIDANT ACTIVITIES OF ALCHEMILLA MOLLIS (BUSER) ROTHM. AND ITS CONTITUENTS; HYPEROSIDE AND ISOQUERCETIN
Küçük Resim Yok
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
University of Ankara
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective: Alchemilla mollis (Buser) Rothm. (Rosaceae) is widely distributed in Europe, North Anatolia, Caucasus, North Iran. A commercial drug, "Herba Alchemillae" is obtained from aerial parts of A. mollis, is used for its astringent, diuretic, antispazmodic properties as well as for treatment of excessive menstruation and wounds in Bulgarian and Turkish folk medicine. Previous studies have reported that hyperoside, isoquercetin, miquelianin, cis-and trans-tiliroside, sinocrassoside D2 and rhodiolgin were detected in A. mollis. Present study is aimed to evaluate in vitro antioxidant effects and cytotoxic activities of A.mollis methanolic extract and its constituents, hyperoside and isoquercetin, on K562 leukemia cell line. Material and Method: Spectrophotometric MTT assay and NO radical scavenging assay have been used to test the cytotoxic effects and antioxidant activities, respectively. Result and Discussion: Results showed that A.mollis methanolic extract reduced cell viability of K562 cells at concentrations higher than 0.02 mg/ml whereas the compounds did not exhibit any cytotoxicity at same concentration range. Additionally, significant inhibition on NO radical production was observed for all tested materials. © 2022 University of Ankara. All rights reserved.
Açıklama
Anahtar Kelimeler
Alchemilla Mollis; Antileukemic Activity; Antioxidant Activity; Hyperoside; Isoquercetin, Hyperin; Isoquercetin; Nitric Oxide; Alchemilla; Alchemilla Mollis; Antileukemic Activity; Antioxidant Activity; Article; Cell Structure; Cell Viability; Drug Cytotoxicity; Free Radical Scavenging Assay; K-562 Cell Line; Mtt Assay; Spectrophotometry
Kaynak
Ankara Universitesi Eczacilik Fakultesi Dergisi
WoS Q Değeri
Scopus Q Değeri
N/A
Cilt
46
Sayı
1
