Molecularly imprinted nanoparticles with recognition properties towards diphtheria toxin for ELISA applications

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dc.authoridONCUL, Mustafa Oral/0000-0002-1681-1866
dc.authoridDal Yontem, Fulya/0000-0003-4767-083X
dc.authoridYasar, Merve/0000-0001-7633-8823
dc.authoridunlu, ayhan/0000-0001-6033-7148
dc.authoridAKCAKAYA, HANDAN/0000-0002-7499-6061
dc.authoridkahraman, memet vezir/0000-0003-1043-6476
dc.authoridKayaman Apohan, Nilhan/0000-0002-7750-3058
dc.authorwosidONCUL, Mustafa Oral/AAE-3429-2020
dc.authorwosidAkçakaya, Handan/AAE-3087-2020
dc.authorwosidunlu, ayhan/Q-1843-2016
dc.contributor.authorAlkanli, Suleyman Serdar
dc.contributor.authorDal Yontem, Fulya
dc.contributor.authorYasar, Merve
dc.contributor.authorGuven, Celal
dc.contributor.authorKahraman, M. Vezir
dc.contributor.authorApohan, Nilhan Kayaman
dc.contributor.authorAktas, Zerrin
dc.date.accessioned2024-06-12T11:08:41Z
dc.date.available2024-06-12T11:08:41Z
dc.date.issued2023
dc.departmentTrakya Üniversitesien_US
dc.description.abstractPlastic antibodies can be used for in vitro neutralization of biomacromolecules with different fragments due to their potential in separation, purification, chemical sensor, catalysis and drug production studies. These polymer nanoparticles with binding affinity and selectivity comparable to natural antibodies were prepared using functional monomer synthesis and copolymerization of acrylic monomers via miniemulsion polymerization. As a result, the in vitro cytotoxic effect from diphtheria toxin was reduced by MIPs. In vitro imaging experiments of polymer nanoparticles (plastic antibodies) were performed to examine the interaction of diphtheria toxin with actin filaments, and MIPs inhibited diphtheria toxin damage on actin filaments. The enzyme-linked immunosorbent assay (ELISA) was performed with plastic antibodies labeled with biotin, and it was determined that plastic antibodies could also be used for diagnostic purposes. We report that molecularly imprinted polymers (MIPs), which are biocompatible polymer nanoparticles, can capture and reduce the effect of diphtheria toxic and its fragment A.en_US
dc.description.sponsorshipScientific and Technological Research Council ofTurkey (TUBITAK) [115S224]; Scientific Research Projects CoordinationUnit of Istanbul University (BAP Project) [25648]en_US
dc.description.sponsorshipFinancial assistance for this research by The Scientific and Technological Research Council ofTurkey (TUBITAK Project No. 115S224); and The Scientific Research Projects CoordinationUnit of Istanbul University (BAP Project No. 25648)en_US
dc.identifier.doi10.1080/09205063.2022.2145866
dc.identifier.endpage767en_US
dc.identifier.issn0920-5063
dc.identifier.issn1568-5624
dc.identifier.issue6en_US
dc.identifier.pmid36357334en_US
dc.identifier.scopus2-s2.0-85142129163en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage753en_US
dc.identifier.urihttps://doi.org/10.1080/09205063.2022.2145866
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22526
dc.identifier.volume34en_US
dc.identifier.wosWOS:000889094600001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofJournal Of Biomaterials Science-Polymer Editionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMolecularly Imprinted Polymeren_US
dc.subjectPlastic Antibodyen_US
dc.subjectDiphtheria Toxinen_US
dc.subjectELISAen_US
dc.subjectCatalytic Domainen_US
dc.subjectTranslocationen_US
dc.subjectProteinsen_US
dc.titleMolecularly imprinted nanoparticles with recognition properties towards diphtheria toxin for ELISA applicationsen_US
dc.typeArticleen_US

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