Fresh broad (Vicia faba) tissue homogenate-based biosensor for determination of phenolic compounds

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dc.authorwosidÖZCAN, Hakkı/AAJ-6857-2021
dc.contributor.authorOzcan, Hakki Mevlut
dc.contributor.authorSagiroglu, Ayten
dc.date.accessioned2024-06-12T10:51:28Z
dc.date.available2024-06-12T10:51:28Z
dc.date.issued2014
dc.departmentTrakya Üniversitesien_US
dc.description.abstractIn this study, a novel fresh broad (Vicia faba) tissue homogenate-based biosensor for determination of phenolic compounds was developed. The biosensor was constructed by immobilizing tissue homogenate of fresh broad (Vicia faba) on to glassy carbon electrode. For the stability of the biosensor, general immobilization techniques were used to secure the fresh broad tissue homogenate in gelatin-glutaraldehyde cross-linking matrix. In the optimization and characterization studies, the amount of fresh broad tissue homogenate and gelatin, glutaraldehyde percentage, optimum pH, optimum temperature and optimum buff er concentration, thermal stability, interference effects, linear range, storage stability, repeatability and sample applications (Wine, beer, fruit juices) were also investigated. Besides, the detection ranges of thirteen phenolic compounds were obtained with the help of the calibration graphs. A typical calibration curve for the sensor revealed a linear range of 5-60 mu M catechol. In reproducibility studies, variation coefficient (CV) and standard deviation (SD) were calculated as 1.59%, 0.64 X 10(-3) M, respectively.en_US
dc.description.sponsorshipTrakya University (Edirne, Turkey) [TUBAB-843]en_US
dc.description.sponsorshipFinancial support for this work was provided by Trakya University (Edirne, Turkey), throughout the projects TUBAB-843.en_US
dc.identifier.doi10.3109/21691401.2013.764313
dc.identifier.endpage261en_US
dc.identifier.issn2169-1401
dc.identifier.issn2169-141X
dc.identifier.issue4en_US
dc.identifier.pmid23363452en_US
dc.identifier.scopus2-s2.0-84904325653en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage256en_US
dc.identifier.urihttps://doi.org/10.3109/21691401.2013.764313
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18375
dc.identifier.volume42en_US
dc.identifier.wosWOS:000339802500005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofArtificial Cells Nanomedicine And Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAmperometric Biosensoren_US
dc.subjectFresh Broaden_US
dc.subjectPhenolic Compounden_US
dc.subjectTissue Biosensoren_US
dc.subjectVicia Fabaen_US
dc.subjectSolid-Phase Extractionen_US
dc.subjectAmperometric Biosensoren_US
dc.subjectLiquid-Chromatographyen_US
dc.subjectElectrodeen_US
dc.subjectCatecholen_US
dc.subjectDetectoren_US
dc.subjectWineen_US
dc.titleFresh broad (Vicia faba) tissue homogenate-based biosensor for determination of phenolic compoundsen_US
dc.typeArticleen_US

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