Fresh broad (Vicia faba) tissue homogenate-based biosensor for determination of phenolic compounds
Küçük Resim Yok
Tarih
2014
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, a novel fresh broad (Vicia faba) tissue homogenate-based biosensor for determination of phenolic compounds was developed. The biosensor was constructed by immobilizing tissue homogenate of fresh broad (Vicia faba) on to glassy carbon electrode. For the stability of the biosensor, general immobilization techniques were used to secure the fresh broad tissue homogenate in gelatin-glutaraldehyde cross-linking matrix. In the optimization and characterization studies, the amount of fresh broad tissue homogenate and gelatin, glutaraldehyde percentage, optimum pH, optimum temperature and optimum buff er concentration, thermal stability, interference effects, linear range, storage stability, repeatability and sample applications (Wine, beer, fruit juices) were also investigated. Besides, the detection ranges of thirteen phenolic compounds were obtained with the help of the calibration graphs. A typical calibration curve for the sensor revealed a linear range of 5-60 mu M catechol. In reproducibility studies, variation coefficient (CV) and standard deviation (SD) were calculated as 1.59%, 0.64 X 10(-3) M, respectively.
Açıklama
Anahtar Kelimeler
Amperometric Biosensor, Fresh Broad, Phenolic Compound, Tissue Biosensor, Vicia Faba, Solid-Phase Extraction, Amperometric Biosensor, Liquid-Chromatography, Electrode, Catechol, Detector, Wine
Kaynak
Artificial Cells Nanomedicine And Biotechnology
WoS Q Değeri
Q4
Scopus Q Değeri
Q1
Cilt
42
Sayı
4
