Protective Effect of Mesna on Intestinal Ischemia- reperfusion Injury by Nitric Oxide and Arginase in an Experimental Rat Model

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dc.authoridUlucam, Enis/0000-0002-4686-7350
dc.authoridCerkezkayabekir, Aysegul/0000-0001-5537-1042;
dc.authorwosidUlucam, Enis/AAG-9204-2019
dc.authorwosidCerkezkayabekir, Aysegul/Q-6629-2018
dc.authorwosidUluçam, Enis/HQZ-3831-2023
dc.contributor.authorFiliz, Sanal
dc.contributor.authorEnis, Ulucam
dc.contributor.authorMustafa, Inan
dc.contributor.authorAysegul, Cerkezkayabekir
dc.contributor.authorElvan, Bakar
dc.date.accessioned2024-06-12T10:52:29Z
dc.date.available2024-06-12T10:52:29Z
dc.date.issued2017
dc.departmentTrakya Üniversitesien_US
dc.description.abstractBackground and Objective: An ischemia-reperfusion (I/R) injury of small intestine is a serious and common condition that is a result of the blockage of the Superior Mesenteric Artery (SMA) because of significant clinical problems. This study aimed to evaluate the effects of the antioxidant mesna on the intestinal nitric oxide (NO) levels and arginase activity in the I/R injury at biochemical and immunohistochemical levels and to determine the tissue malondialdehyde (MDA) level as a marker of oxidative damage. Methodology: In the study, 10-12 week-old male Wistar albino rats weighing 200-250 g were used. The animals were grouped as group A-Sham-control group, group B-Intestinal I/R group and group C-mesna (n = 7). The I/R were performed by binding and opening the SMA after 120 min. In the mesna group, mesna (150 mg kg(-1), ip) was given immediately after opening the SMA. Following surgical procedures, the inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) were shown in the tissue. The levels of NO and MDA and the activity of arginase were measured. The levels of MDA and the NO, arginase activity, immunoreactivity of iNOS and eNOS were compared to the control group. Statistical analysis was done using SPSS 19 and descriptive statistics were expressed as arithmetic Mean +/- Standard deviation. Results: It was observed that the values were significantly increased (p<0.05) in the I/R group and decreased in the mesna group (p<0.05). Mesna has demonstrated its significant therapeutic effects on biochemical parameters in the I/R process by decreasing the NO level, equilibrating the iNOS and eNOS expressions and decreasing the arginase activity. Conclusion: It has been observed that mesna was helpful against the intestinal I/R injury because of its antioxidant properties considering the biochemical parameters. Mesna has demonstrated its significant therapeutic effects on biochemical parameters in the I/R process by decreasing the NO level, equilibrating the iNOS and eNOS expressions and decreasing the arginase activity.en_US
dc.description.sponsorship[TUBAP-2010-147]en_US
dc.description.sponsorshipThis was a part of study of research project number TUBAP-2010-147 supported Trakya University Foundation of Scientific Research.en_US
dc.identifier.doi10.3923/ijp.2017.1038.1046
dc.identifier.endpage1046en_US
dc.identifier.issn1811-7775
dc.identifier.issn1812-5700
dc.identifier.issue8en_US
dc.identifier.scopus2-s2.0-85032376439en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage1038en_US
dc.identifier.urihttps://doi.org/10.3923/ijp.2017.1038.1046
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18720
dc.identifier.volume13en_US
dc.identifier.wosWOS:000412986900011en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherAsian Network Scientific Information-Ansineten_US
dc.relation.ispartofInternational Journal Of Pharmacologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectArginaseen_US
dc.subjectIschemia-Reperfusionen_US
dc.subjectIntestineen_US
dc.subjectMesnaen_US
dc.subjectNitric Oxideen_US
dc.subjectSynthaseen_US
dc.subjectInflammatory-Bowel-Diseaseen_US
dc.subjectArtery-Occlusion Shocken_US
dc.subjectIschemia/Reperfusion Injuryen_US
dc.subjectOxidative Stressen_US
dc.subject2-Mercaptoethane Sulfonateen_US
dc.subjectInos Expressionsen_US
dc.subjectDamageen_US
dc.subjectSynthaseen_US
dc.subjectKidneyen_US
dc.subjectLiveren_US
dc.titleProtective Effect of Mesna on Intestinal Ischemia- reperfusion Injury by Nitric Oxide and Arginase in an Experimental Rat Modelen_US
dc.typeArticleen_US

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