Protective Effect of Mesna on Intestinal Ischemia- reperfusion Injury by Nitric Oxide and Arginase in an Experimental Rat Model
Küçük Resim Yok
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Asian Network Scientific Information-Ansinet
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background and Objective: An ischemia-reperfusion (I/R) injury of small intestine is a serious and common condition that is a result of the blockage of the Superior Mesenteric Artery (SMA) because of significant clinical problems. This study aimed to evaluate the effects of the antioxidant mesna on the intestinal nitric oxide (NO) levels and arginase activity in the I/R injury at biochemical and immunohistochemical levels and to determine the tissue malondialdehyde (MDA) level as a marker of oxidative damage. Methodology: In the study, 10-12 week-old male Wistar albino rats weighing 200-250 g were used. The animals were grouped as group A-Sham-control group, group B-Intestinal I/R group and group C-mesna (n = 7). The I/R were performed by binding and opening the SMA after 120 min. In the mesna group, mesna (150 mg kg(-1), ip) was given immediately after opening the SMA. Following surgical procedures, the inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) were shown in the tissue. The levels of NO and MDA and the activity of arginase were measured. The levels of MDA and the NO, arginase activity, immunoreactivity of iNOS and eNOS were compared to the control group. Statistical analysis was done using SPSS 19 and descriptive statistics were expressed as arithmetic Mean +/- Standard deviation. Results: It was observed that the values were significantly increased (p<0.05) in the I/R group and decreased in the mesna group (p<0.05). Mesna has demonstrated its significant therapeutic effects on biochemical parameters in the I/R process by decreasing the NO level, equilibrating the iNOS and eNOS expressions and decreasing the arginase activity. Conclusion: It has been observed that mesna was helpful against the intestinal I/R injury because of its antioxidant properties considering the biochemical parameters. Mesna has demonstrated its significant therapeutic effects on biochemical parameters in the I/R process by decreasing the NO level, equilibrating the iNOS and eNOS expressions and decreasing the arginase activity.
Açıklama
Anahtar Kelimeler
Arginase, Ischemia-Reperfusion, Intestine, Mesna, Nitric Oxide, Synthase, Inflammatory-Bowel-Disease, Artery-Occlusion Shock, Ischemia/Reperfusion Injury, Oxidative Stress, 2-Mercaptoethane Sulfonate, Inos Expressions, Damage, Synthase, Kidney, Liver
Kaynak
International Journal Of Pharmacology
WoS Q Değeri
Q4
Scopus Q Değeri
N/A
Cilt
13
Sayı
8
