Sunflower seed lipase: Extraction, purification, and characterization

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dc.contributor.authorSagiroglu, A
dc.contributor.authorArabaci, N
dc.date.accessioned2024-06-12T11:07:40Z
dc.date.available2024-06-12T11:07:40Z
dc.date.issued2005
dc.departmentTrakya Üniversitesien_US
dc.description.abstractA simple procedure for the extraction of the lipolytic activity from sunflower seed has been developed. Various conditions of extraction have been optimized in order to obtain maximum yield of lipase. A new lipase enzyme was purified by the fractional salt precipitation from the supernatant, dialysis on a cellulose membrane. and gel column chromatography on Sephadex G-75. The lipase was monomeric. with an apparent M, of 22 kDa and a pI of 8, with the electrophoretic analysis. Kinetics of the enzyme activity versus substrate concentration showed typical lipase behavior. with K-m and V-max values of 1.33 mM and 555 U/mg. All trialycerides were efficiently hydrolyzed by the enzyme, but this showed a preference towards triglycerides of natural mono unsaturated fatty acids. The optimum temperature. pH. and incubation time for lipolytic activity were 50degreesC, 7.5, and 5 min, respectively. The stability of the sunflower lipase was investigated under operational and storage conditions. It was found that this enzyme preserved its lipolytic activity at temperatures between at 35-50degreesC, alkaline pH, and for a period of about four months.en_US
dc.identifier.doi10.1081/PB-200041442
dc.identifier.endpage51en_US
dc.identifier.issn1082-6068
dc.identifier.issn1532-2297
dc.identifier.issue1en_US
dc.identifier.pmid15704496en_US
dc.identifier.scopus2-s2.0-12444328796en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage37en_US
dc.identifier.urihttps://doi.org/10.1081/PB-200041442
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22138
dc.identifier.volume35en_US
dc.identifier.wosWOS:000226591200004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofPreparative Biochemistry & Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLipase Purificationen_US
dc.subjectCharacterizationen_US
dc.subjectSunflower Seeden_US
dc.subjectHeliantus Annuus L.en_US
dc.subjectSubstrate Specificationen_US
dc.subjectRice Bran Lipaseen_US
dc.subjectFatty-Acidsen_US
dc.subjectCastor-Oilen_US
dc.subjectResolutionen_US
dc.subjectGlycerolen_US
dc.titleSunflower seed lipase: Extraction, purification, and characterizationen_US
dc.typeArticleen_US

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