Sunflower seed lipase: Extraction, purification, and characterization
Küçük Resim Yok
Tarih
2005
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A simple procedure for the extraction of the lipolytic activity from sunflower seed has been developed. Various conditions of extraction have been optimized in order to obtain maximum yield of lipase. A new lipase enzyme was purified by the fractional salt precipitation from the supernatant, dialysis on a cellulose membrane. and gel column chromatography on Sephadex G-75. The lipase was monomeric. with an apparent M, of 22 kDa and a pI of 8, with the electrophoretic analysis. Kinetics of the enzyme activity versus substrate concentration showed typical lipase behavior. with K-m and V-max values of 1.33 mM and 555 U/mg. All trialycerides were efficiently hydrolyzed by the enzyme, but this showed a preference towards triglycerides of natural mono unsaturated fatty acids. The optimum temperature. pH. and incubation time for lipolytic activity were 50degreesC, 7.5, and 5 min, respectively. The stability of the sunflower lipase was investigated under operational and storage conditions. It was found that this enzyme preserved its lipolytic activity at temperatures between at 35-50degreesC, alkaline pH, and for a period of about four months.
Açıklama
Anahtar Kelimeler
Lipase Purification, Characterization, Sunflower Seed, Heliantus Annuus L., Substrate Specification, Rice Bran Lipase, Fatty-Acids, Castor-Oil, Resolution, Glycerol
Kaynak
Preparative Biochemistry & Biotechnology
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
35
Sayı
1
