IDENTIFICATION OF LENS CULTIVARS IN MARKET BY MOLECULAR TOOLS: DNA BARCODING AND SSRs

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dc.contributor.authorTatlises, Metin Burak
dc.contributor.authorHasancebi, Semra
dc.date.accessioned2024-06-12T11:02:06Z
dc.date.available2024-06-12T11:02:06Z
dc.date.issued2023
dc.departmentTrakya Üniversitesien_US
dc.description.abstractSubstitution of plant cultivars of high commercial value with a cheaper, lower quality one is a common fraud committed against consumers and producers. Since it is one of the most widely grown legumes, lentil (Lens culinaris Medik.) is suitable for such frauds. This study aimed to identify lentil cultivars which are registered and authorized in the market in Turkiye by using current molecular methods. For this purpose, 26 lentil cultivars were analyzed for 15 SSR markers and two DNA barcode regions (trnH-psbA and matK). A high allele diversity was observed by 12 scorable SSR markers, and the average number of alleles was determined to be 16. One of the important findings was the presence of cultivar-specific alleles that can be used to identify each cultivar in the lentil market in Turkiye. At least one cultivar-specific allele was obtained for each cultivar. The lentil cultivars were also analyzed by two DNA barcode regions as trnH-psbA and matK. While it was observed that the rate of the intra-species variation for the trnH-psbA region was low and 26 varieties were divided into 7 groups, higher rate was found for matK and samples were distributed into 14 groups. Nevertheless, it was observed that intra-species discrimination can be made more effective when both loci are used together and 26 species were distributed into 18 different groups. We expect that the results of this study, especially the cultivar-specific SSR alleles and DNA barcoding sequence data may be used routinely to identify production and packaged products that are commercially available in markets.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkiye (TUBITAK) [218O172]en_US
dc.description.sponsorshipThe study was supported by the Scientific and Technological Research Council of Turkiye (TUBITAK) with project number 218O172.en_US
dc.identifier.doi10.23902/trkjnat.1324202
dc.identifier.endpage100en_US
dc.identifier.issn2528-9691
dc.identifier.issue2en_US
dc.identifier.startpage91en_US
dc.identifier.trdizinid1201962en_US
dc.identifier.urihttps://doi.org/10.23902/trkjnat.1324202
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1201962
dc.identifier.urihttps://hdl.handle.net/20.500.14551/21153
dc.identifier.volume24en_US
dc.identifier.wosWOS:001088177900011en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherTrakya Univ Balkan Yerlesesi Enstituler Binasien_US
dc.relation.ispartofTrakya University Journal Of Natural Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectFood Traceabilityen_US
dc.subjectDNA Barcodingen_US
dc.subjectSSRen_US
dc.subjectLens Culinarisen_US
dc.subjectMicrosatelliteen_US
dc.subjectAdulterationen_US
dc.subjectRegionsen_US
dc.subjectHrmen_US
dc.subjectAuthenticationen_US
dc.subjectSequencesen_US
dc.subjectTortoiseen_US
dc.subjectUtilityen_US
dc.titleIDENTIFICATION OF LENS CULTIVARS IN MARKET BY MOLECULAR TOOLS: DNA BARCODING AND SSRsen_US
dc.typeArticleen_US

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Trakya University Journal of Natural Sciences