A new electrochemical impedance biosensor based on aromatic thiol for alpha-1 antitrypsin determination

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dc.authoridOZCAN, Hakki/0000-0003-2204-7267
dc.authoridMEHMET, OSMAN/0000-0001-6679-1907
dc.contributor.authorYagar, Huelya
dc.contributor.authorOzcan, Hakki Mevluet
dc.contributor.authorMehmet, Osman
dc.date.accessioned2024-06-12T10:51:19Z
dc.date.available2024-06-12T10:51:19Z
dc.date.issued2021
dc.departmentTrakya Üniversitesien_US
dc.description.abstractAlpha-1 antitrypsin (A1AT) is one of the acute phase proteins which are synthesized in the liver. A1AT inhibits the activity of many proteases, but its main task is to protect the lungs from the attack of neutrophil elastase. In an autosomal hereditary disease known as alpha-1 antitrypsin deficiency, the A1AT level in blood serum decreases, increasing the risk of developing emphysema, liver apoptosis, and liver cancer. Thus, the detection of A1AT concentration in blood serum is very important. In this study, an impedimetric biosensor was developed, forming an SAM (self-assembled monolayer) with 4-mercaptophenylacetic acid (4MPA) on the surface of the gold electrode. An A1AT biosensor was constructed using immobilization of an A1AT-specific antibody (anti-A1AT) after activating the carboxyl groups of 4MPA with EDC/NHS. Each immobilization stage was characterized by using electrochemical impedance spectroscopy, cyclic voltammetry, and scanning electron microscopy with energy dispersive X-ray spectroscopy. With the designed biosensor, precise and highly reproducible results were obtained for A1AT concentrations in the range of 100-600 mu g/mL. A1AT detection was also successfully carried out in artificial serum solutions spiked with A1AT.en_US
dc.description.sponsorshipTrakya University Scientific Research Foundation [TUBAP-2017/145]en_US
dc.description.sponsorshipThis study was supported by Trakya University Scientific Research Foundation (Project Number: TUBAP-2017/145).en_US
dc.identifier.doi10.3906/kim-2007-6
dc.identifier.endpage118en_US
dc.identifier.issn1300-0527
dc.identifier.issue1en_US
dc.identifier.pmid33679157en_US
dc.identifier.startpage104en_US
dc.identifier.trdizinid525654en_US
dc.identifier.urihttps://doi.org/10.3906/kim-2007-6
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/525654
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18309
dc.identifier.volume45en_US
dc.identifier.wosWOS:000639238600011en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal Of Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAlpha-1 Antitrypsinen_US
dc.subjectElectrochemical Immunosensoren_US
dc.subject4-Mercaptophenylacetic Aciden_US
dc.subjectPulmonary Emphysemaen_US
dc.subjectAlzheimer's Diseaseen_US
dc.subjectSelf-Assembled Monolayersen_US
dc.subjectEnzymatic Biosensoren_US
dc.subjectGold Electrodeen_US
dc.subjectImmunosensoren_US
dc.subjectSerumen_US
dc.subjectDeficiencyen_US
dc.subjectBiomarkeren_US
dc.subjectPolymeren_US
dc.titleA new electrochemical impedance biosensor based on aromatic thiol for alpha-1 antitrypsin determinationen_US
dc.typeArticleen_US

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