A new electrochemical impedance biosensor based on aromatic thiol for alpha-1 antitrypsin determination
Küçük Resim Yok
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Tubitak Scientific & Technological Research Council Turkey
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Alpha-1 antitrypsin (A1AT) is one of the acute phase proteins which are synthesized in the liver. A1AT inhibits the activity of many proteases, but its main task is to protect the lungs from the attack of neutrophil elastase. In an autosomal hereditary disease known as alpha-1 antitrypsin deficiency, the A1AT level in blood serum decreases, increasing the risk of developing emphysema, liver apoptosis, and liver cancer. Thus, the detection of A1AT concentration in blood serum is very important. In this study, an impedimetric biosensor was developed, forming an SAM (self-assembled monolayer) with 4-mercaptophenylacetic acid (4MPA) on the surface of the gold electrode. An A1AT biosensor was constructed using immobilization of an A1AT-specific antibody (anti-A1AT) after activating the carboxyl groups of 4MPA with EDC/NHS. Each immobilization stage was characterized by using electrochemical impedance spectroscopy, cyclic voltammetry, and scanning electron microscopy with energy dispersive X-ray spectroscopy. With the designed biosensor, precise and highly reproducible results were obtained for A1AT concentrations in the range of 100-600 mu g/mL. A1AT detection was also successfully carried out in artificial serum solutions spiked with A1AT.
Açıklama
Anahtar Kelimeler
Alpha-1 Antitrypsin, Electrochemical Immunosensor, 4-Mercaptophenylacetic Acid, Pulmonary Emphysema, Alzheimer's Disease, Self-Assembled Monolayers, Enzymatic Biosensor, Gold Electrode, Immunosensor, Serum, Deficiency, Biomarker, Polymer
Kaynak
Turkish Journal Of Chemistry
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
45
Sayı
1
