Edirne Selimiye Camii Kütüphanesi'nin iç ve dış havasındaki mikrofunguslar
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Dosyalar
Tarih
2007
Yazarlar
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
EDiRNE SEL M YE CAM KÜTÜPHANES N N Ç VE DIŞ HAVASINDAK M KROFUNGUSLAR Bu çalışmada, Selimiye camii kütüphanesinde bulunan 15., 16., ve 17.yy dan kalma el yazması eserlerde karşılaşılan mikrobiyoloji kökenli bozulmalara neden olan mikrofungusların tespiti; aylık, mevsimsel ve meteorolojik faktörlerle ilişkisi ve alınacak önlemlerin belirlenmesi amaçlanmıştır. Bu amaçla kütüphanenin 2 farklı iç ortamından, dış ortamından ve el yazması eserlerden Nisan 2006- Eylül 2006 tarihlerini kapsayan 6 aylık sürede ayda 1 defa olmak üzere örnekleme işlemi yapılmıştır. ç ve dış ortam atmosferinden örnek almada ?Yerçekimine Dayalı Petri Plak Metodu? kullanılmıştır. Bu metotta içlerinde mikrofunguslar için Rose-Bengal ve Streptomycin ilaveli Pepton Dekstroz Agar besiyerlerinin bulunduğu petri plaklarının hava ile temas ettirilmesi sağlanmak suretiyle ( ç mekânlarda 30 dk. Dış ortamda 20 dk) örnekleme işlemi yapılmaktadır. El Yazması Eserlerden örnek almada ise steril eküvyon çubuklar kullanılmıştır. Gözle yapılan incelemede mikrofungus üremesi olan yada şüphelenilen bölgelerden steril eküvyon çubuklarla örnekler alınıp yine steril şartlara uygun olacak şekilde içlerinde Rose-Bengal ve Streptomycin ilaveli Pepton Dekstroz Agar bulunan besiyerlerinin bulunduğu petri plaklarına ekilmiştir. Mikrofungus izolasyonu için toplam 140 petri plağı kullanılmış ve bu plaklarda 322' si iç ortamdan, 114' ü dış ortamdan ve 1350' si eserlerden olmak üzere toplam 1786 mikrofungus kolonisi sayılmıştır. Çalışma sonucunda 19 mikrofungus cinsi ( Alternaria, Penicillium, Ulocladium, Scopulariopsis, Cladosporium, Drechslera, Mycotypha, Aspergillus, Trichoderma, Chaetomium, Rhizopus, Fusarium, Acremonium, Botrytis, Stemphylium, Mucor, Epicoccum, Phoma, Curvularia) tespit edilmiştir. Alternaria, Cladosporium, Penicillium en sık izole edilen mikrofungus cinsleridir. Araştırma süresince her ay Alternaria, Cladosporium, Penicillium, Aspergillus, Rhizopus, Ulocladium cinsleri ve Ssporsuz mikrofunguslar bulunmuştur. Anahtar Kelimeler: Mikrofungus, Kağıt Yapısında Meydana Lekelenmeler, Kâğıt Konservasyonu, Biyolojik bozunma.
INDOOR AND OUTDOOR AIRBORNE MICROFUNGI IN LIBRARY OF EDIRNE SELIMIYE MOSQUE This study was performed in order to determine the microfungi leading to decomposition of hand-written books dating back to 15th, 16th and 17th centuries and kept in Selimiye Mosque library, to reveal their correlations with monthly, seasonal and meteorological factors and to report possible measurements to be taken. For this purpose, samples were taken from the library once in a month for a 6 months period between April 2006 and September 2006, from two different indoor airs, from outdoor air and from the books. ?Gravitational Sampling Method? was used for indoor and outdoor samplings. For this method, petri plates including Rose-Bengal and Streptomycin added Peptone Dextrose Agar media were exposed to air for 30 minutes in indoor air and for 20 minutes in outdoor air. A sterile swab was used for samplings from books. Swabbing was done over places of the books where a microfungal growth existed or it was possible to exist, and samples were inoculated in sterile conditions into petri plates including Rose-Bengal and Streptomycin added Peptone Dextrose Agar media The 140 petri plates were used for microfungi isolation and total 1786 mikrofungi ( 322 of indoor, 114 of outdoor and 1350 of work of arts) colonies were counted in these plates. As a result of this study, 19 microfungal genera (Alternaria, Penicillium, Ulocladium, Scopulariopsis, Cladosporium, Drechslera, Mycotypha, Aspergillus, Trichoderma, Chaetomium, Rhizopus, Fusarium, Acremonium, Botrytis, Stemphylium, Mucor, Epicoccum, Phoma, Curvularia) were isolated. Most populous microfungi were Alternaria, Cladosporium and Penicillium. During sampling, Alternaria, Cladosporium, Penicillium, Aspergillus, Rhizopus, Ulocladium and non-sporulating microfungi were isolated every months. Keywords: Microfungi, Foxing, Paper Conservation, Biodeterioration
INDOOR AND OUTDOOR AIRBORNE MICROFUNGI IN LIBRARY OF EDIRNE SELIMIYE MOSQUE This study was performed in order to determine the microfungi leading to decomposition of hand-written books dating back to 15th, 16th and 17th centuries and kept in Selimiye Mosque library, to reveal their correlations with monthly, seasonal and meteorological factors and to report possible measurements to be taken. For this purpose, samples were taken from the library once in a month for a 6 months period between April 2006 and September 2006, from two different indoor airs, from outdoor air and from the books. ?Gravitational Sampling Method? was used for indoor and outdoor samplings. For this method, petri plates including Rose-Bengal and Streptomycin added Peptone Dextrose Agar media were exposed to air for 30 minutes in indoor air and for 20 minutes in outdoor air. A sterile swab was used for samplings from books. Swabbing was done over places of the books where a microfungal growth existed or it was possible to exist, and samples were inoculated in sterile conditions into petri plates including Rose-Bengal and Streptomycin added Peptone Dextrose Agar media The 140 petri plates were used for microfungi isolation and total 1786 mikrofungi ( 322 of indoor, 114 of outdoor and 1350 of work of arts) colonies were counted in these plates. As a result of this study, 19 microfungal genera (Alternaria, Penicillium, Ulocladium, Scopulariopsis, Cladosporium, Drechslera, Mycotypha, Aspergillus, Trichoderma, Chaetomium, Rhizopus, Fusarium, Acremonium, Botrytis, Stemphylium, Mucor, Epicoccum, Phoma, Curvularia) were isolated. Most populous microfungi were Alternaria, Cladosporium and Penicillium. During sampling, Alternaria, Cladosporium, Penicillium, Aspergillus, Rhizopus, Ulocladium and non-sporulating microfungi were isolated every months. Keywords: Microfungi, Foxing, Paper Conservation, Biodeterioration
Açıklama
Anahtar Kelimeler
Biyoloji, Biology, Mikrobiyoloji, Microbiology, Müzecilik, Museology
