Hepatobiliary scintigraphy for evaluating the hepatotoxic effect of halothane and the protective effect of catechin in comparison with histo-chemical analysis of liver tissue

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dc.authoridSalihoglu, Yavuz Sami/0000-0003-2465-9128
dc.contributor.authorKaramanlioglu, B
dc.contributor.authorYüksel, M
dc.contributor.authorTemiz, E
dc.contributor.authorSalihoglu, YS
dc.contributor.authorÇiftçi, S
dc.date.accessioned2024-06-12T11:02:53Z
dc.date.available2024-06-12T11:02:53Z
dc.date.issued2002
dc.departmentTrakya Üniversitesien_US
dc.description.abstractHalothane and its metabolites cause liver damage by decreasing liver blood flow and generating free-radical species. Catechin suppresses lipid peroxidation and increases enzyme activity, therefore it seems to be capable of protecting liver parenchyma against tine direct toxic effect of halothane. The aim of this study was to investigate the role of hepatobiliary scintigraphy in detecting liver damage after halothane anaesthesia and the protective effect of catechin in comparison with histo-chemical analysis. Thirty rabbits, divided into three groups (A, controls; B, halothane; and C, catechin+halothane), were investigated. In group A no anaesthesia was administered. Group B only received halothane, while group C was pretreated with catechin and halothane anaesthesia was administered for 2 h. Dynamic scintigrams were taken for 60 min after injecting Tc-99m-mebrofenin, and the time of peak uptake (TPU) and the time for half of the activity to clear from the liver (T-1/2) were calculated. Rabbits were killed, and malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) levels were measured in hepatic tissue. The TPU and T-1/2 values of group A is significantly lower than in groups B and C (P < 0.0002 and P < 0.0002, respectively, for TPU; and P < 0.0002 and P < 0.0003, respectively, for T-1/2). The TPU and T-1/2 values of group B were significantly higher than in group C (P < 0.0003 and P < 0.0003, respectively). The hepatic MDA level of group A was significantly lower than in groups B and C (P < 0.0002 and P < 0.0002, respectively). SOD, GSH-Px and CAT levels of group A were significantly higher than in groups B and C (P < 0.0002, P < 0.0001 and P < 0.003, respectively, for group A vs group B; and P < 0.0005, P < 0.0002 and P < 0.03, respectively, for group A vs group C). The MDA level of group B was significantly higher than that in group C (P < 0.0002). SOD, GSH-Px and CAT levels of group B were significantly lower than in group C (P < 0.0002, P < 0.0002 and P < 0.003, respectively). According to these results, we suggest that catechin protects liver parenchyma against the toxic effect of halothane and its metabolites, and that, compared to invasive histo-chemical analysis, hepatobiliary scintigraphy is a useful and alternative noninvasive method for detecting the protective effect of catechin on liver parenchyma after halothane anaesthesia. ((C) 2002 Lippincott Williams & Wilkins).en_US
dc.identifier.doi10.1097/00006231-200201000-00009
dc.identifier.endpage59en_US
dc.identifier.issn0143-3636
dc.identifier.issn1473-5628
dc.identifier.issue1en_US
dc.identifier.pmid11748438en_US
dc.identifier.scopus2-s2.0-0036184993en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage53en_US
dc.identifier.urihttps://doi.org/10.1097/00006231-200201000-00009
dc.identifier.urihttps://hdl.handle.net/20.500.14551/21456
dc.identifier.volume23en_US
dc.identifier.wosWOS:000172991600009en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherLippincott Williams & Wilkinsen_US
dc.relation.ispartofNuclear Medicine Communicationsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHalothaneen_US
dc.subjectCatechinen_US
dc.subjectMebrofeninen_US
dc.subjectRadical Oxygen Speciesen_US
dc.subjectHisto-Chemical Analysisen_US
dc.subjectLipid-Peroxidationen_US
dc.subjectFree-Radicalsen_US
dc.subjectIschemia-Reperfusionen_US
dc.subjectAntioxidant Activityen_US
dc.subjectHepatic-Functionen_US
dc.subjectInjuryen_US
dc.subjectPlasmaen_US
dc.subjectRatsen_US
dc.subjectQuantificationen_US
dc.subjectAnestheticsen_US
dc.titleHepatobiliary scintigraphy for evaluating the hepatotoxic effect of halothane and the protective effect of catechin in comparison with histo-chemical analysis of liver tissueen_US
dc.typeArticleen_US

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