Protective effects of L-arginine on rat terminal ileum subjected to ischemia/reperfusion
Küçük Resim Yok
Tarih
2008
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Lippincott Williams & Wilkins
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objectives: Studies have shown that nitric oxide (NO) may play a major role in sustaining mucosal integrity; however, NO has been also implicated in the pathogenesis of ischemia/ reperfusion (I/R)-related tissue injury. We investigated the effects of L-arginine and N-G-nitro L-arginine methyl ester (L-NAME) on the acetylcholine-induced contractile response of ileum and the levels of malondialdehyde (MDA) and reduced glutathione (GSH). Histopathological changes were also evaluated in ileal preparations. Materials and Methods: Mate Wistar Albino rats were subjected to mesenteric ischemia (30 min) followed by reperfusion (3 hours). Four groups were designed: sham-operated control; I/R; I/R and L-arginine pretreatment; and I/ R and L-NAME pretreatment. After reperfusion, ileum specimens were collected to determine the parameters mentioned above. Results: Following reperfusion, a significant decrease in acetylcholine-induced contractile response, an increase in lipid peroxidation, a decrease in GSH content, and mucosal damage of the ileal preparations were observed. We showed that decreased contractility, increased lipid peroxidation, and reduced GSH content have been reversed by L-arginine but not by L-NAME. Mucosal injury was significantly lowered in the L-arginine group. Conclusions: Treatment with L-arginine exerted a protective effect in intestinal I/R injury, which was mediated in part by regulating MDA and GSH levels, consequently ameliorating impaired contractile response and mucosal injury.
Açıklama
Anahtar Kelimeler
Contractile Activity, Ileum, Ischemia And Reperfusion, Nitric Oxide, Nitric-Oxide Synthase, Intestinal Ischemia-Reperfusion, Ileal Longitudinal Muscle, Injury, Model, Responsiveness, Motility
Kaynak
Journal Of Pediatric Gastroenterology And Nutrition
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
46
Sayı
1
