Protective effects of L-arginine on rat terminal ileum subjected to ischemia/reperfusion
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Date
2008
Journal Title
Journal ISSN
Volume Title
Publisher
Lippincott Williams & Wilkins
Access Rights
info:eu-repo/semantics/openAccess
Abstract
Objectives: Studies have shown that nitric oxide (NO) may play a major role in sustaining mucosal integrity; however, NO has been also implicated in the pathogenesis of ischemia/ reperfusion (I/R)-related tissue injury. We investigated the effects of L-arginine and N-G-nitro L-arginine methyl ester (L-NAME) on the acetylcholine-induced contractile response of ileum and the levels of malondialdehyde (MDA) and reduced glutathione (GSH). Histopathological changes were also evaluated in ileal preparations. Materials and Methods: Mate Wistar Albino rats were subjected to mesenteric ischemia (30 min) followed by reperfusion (3 hours). Four groups were designed: sham-operated control; I/R; I/R and L-arginine pretreatment; and I/ R and L-NAME pretreatment. After reperfusion, ileum specimens were collected to determine the parameters mentioned above. Results: Following reperfusion, a significant decrease in acetylcholine-induced contractile response, an increase in lipid peroxidation, a decrease in GSH content, and mucosal damage of the ileal preparations were observed. We showed that decreased contractility, increased lipid peroxidation, and reduced GSH content have been reversed by L-arginine but not by L-NAME. Mucosal injury was significantly lowered in the L-arginine group. Conclusions: Treatment with L-arginine exerted a protective effect in intestinal I/R injury, which was mediated in part by regulating MDA and GSH levels, consequently ameliorating impaired contractile response and mucosal injury.
Description
Keywords
Contractile Activity, Ileum, Ischemia And Reperfusion, Nitric Oxide, Nitric-Oxide Synthase, Intestinal Ischemia-Reperfusion, Ileal Longitudinal Muscle, Injury, Model, Responsiveness, Motility
Journal or Series
Journal Of Pediatric Gastroenterology And Nutrition
WoS Q Value
Q2
Scopus Q Value
Q1
Volume
46
Issue
1
