Genotoxic and Cytotoxic Evaluation of Pyrethroid Insecticides ?-Cyhalothrin and ?-Cypermethrin on Human Blood Lymphocyte Culture
Küçük Resim Yok
Tarih
2013
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The present study aimed to investigate the genotoxic, cytotoxic and aneugenic effects of 1, 2, 3.75, 7.5, 15, 30 mu M concentrations of the insecticides lambda-cyhalothrin (LCT) and alpha-cypermethrin (CYP) on human peripheral blood lymphocyte culture using micronucleus (MN) and fluorescence in situ hybridisation (FISH) methods. All the concentrations were tested to assess the MN and apoptosis effects, and 1 and 2 mu M LCT and 7.5 and 15 mu M CYP concentrations were tested for FISH analysis. The cytotoxic effect was also observed using trypan blue and the acridine orange/ethidium bromide fluorescence staining method to measure the apoptotic effect. It was observed that both of the insecticides had a cytotoxic effect at all the concentrations (p a parts per thousand currency sign 0.001) and apoptotic effect for LCT at 15-30 mu M (p a parts per thousand currency sign 0.05; p a parts per thousand currency sign 0.01) for CYP between 2 and 30 mu M concentrations (p a parts per thousand currency sign 0.05; p a parts per thousand currency sign 0.01). The micronuclei that developed after exposure were induced because of an aneugenic effect (p a parts per thousand currency sign 0.001). LCT and CYP might be spindle poisons or caused damaged to centromere/kinetochore function.
Açıklama
Anahtar Kelimeler
Lambda-Cyhalothrin, Alpha-Cypermethrin, FISH, Aneugenic Effect, In-Vitro, Induction, Micronuclei, Pesticides, Aneuploidy, Vivo
Kaynak
Bulletin Of Environmental Contamination And Toxicology
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
90
Sayı
3
