A morphological study of uterine alterations in mice due to exposure to cadmium
Küçük Resim Yok
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
We investigated the morphologic and molecular effects of exposure to cadmium (Cd) for 30 and 60 days on the uteri of mice. We assessed uterine morphometric measurements, eosinophilia, mast cell numbers, endometrial apoptosis, proliferation and estrogen receptor alpha (ER) immunoreactivity. We examined vaginal smears that reflected the hormonal alterations in the female reproductive tract. Because the female reproductive tract exhibits different morphology at each stage of the estrous cycle, we sacrificed all animals at estrus to make appropriate comparisons. Female BALB/c mice were exposed to 200 ppm Cd in their drinking water for either 30 or 60 days. Cd exposure caused significant decreases in endometrial thickness and number of glands in estrus phase uteri. The endometrial eosinophilia in the groups exposed to Cd also decreased compared to controls. Cd exposure increased the number of mast cells. Luminal and glandular epithelia were examined using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and by immunostaining proliferating cell nuclear antigen (PCNA) and estrogen receptor (ER). Compared to controls, the apoptotic index increased with time in both Cd exposed groups, while the proliferation index decreased. ER immunoreactivity was decreased in both Cd exposed groups compared to controls; the decrease was most apparent in the 30 day Cd group. We found that 60 day Cd exposure increased apoptosis in the endometrium, which may affect the receptivity of the uterus for implantation.
Açıklama
Anahtar Kelimeler
Apoptosis, Cadmium, Estrogen Receptor, Mice, Proliferating Cell Nuclear Antigen, Uterus, Oxidative Stress, Estrogen-Receptor, Rat Uterus, In-Vivo, Renal Toxicity, Mast-Cells, Pathway, Mechanism, Kinase, Death
Kaynak
Biotechnic & Histochemistry
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
92
Sayı
4
