Entrapment of laurel lipase in chitosan hydrogel beads

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dc.contributor.authorYagar, Hulya
dc.contributor.authorBalkan, Ugur
dc.date.accessioned2024-06-12T10:56:20Z
dc.date.available2024-06-12T10:56:20Z
dc.date.issued2017
dc.departmentTrakya Üniversitesien_US
dc.description.abstractLaurel seed lipase was entrapped within chitosan beads with ionotropic gelatin method using tripolyphosphate (TPP) as multivalent covalent counter ion. Immobilization yield was 78%. First, optimum immobilization conditions were determined, and morphology of chitosan beads was characterized by scanning electron microscopy. Optimum pH and temperature were evaluated as 6.0 and 40 degrees C, respectively. The immobilized beads saved about 55% of its activities at 60 degrees while saved about 32% at 70 degrees C for 30min. V-max/K-m values were determined as 31.75 and 2.87 using olive oil as substrate for immobilized beads and free enzyme, respectively. Immobilized beads showed the activities during 30 days at+4 degrees C.en_US
dc.description.sponsorshipResearch Fund of Trakya University [TUBAP-2011/157]en_US
dc.description.sponsorshipThis research was funded by Research Fund of Trakya University (TUBAP-2011/157).en_US
dc.identifier.doi10.1080/21691401.2016.1182920
dc.identifier.endpage870en_US
dc.identifier.issn2169-1401
dc.identifier.issn2169-141X
dc.identifier.issue5en_US
dc.identifier.pmid27181370en_US
dc.identifier.scopus2-s2.0-84967205762en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage864en_US
dc.identifier.urihttps://doi.org/10.1080/21691401.2016.1182920
dc.identifier.urihttps://hdl.handle.net/20.500.14551/19761
dc.identifier.volume45en_US
dc.identifier.wosWOS:000402039400002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofArtificial Cells Nanomedicine And Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectChitosanen_US
dc.subjectImmobilizationen_US
dc.subjectIonotropic Gelatin Methoden_US
dc.subjectLaurelen_US
dc.subjectLipaseen_US
dc.subjectTripolyphosphateen_US
dc.subjectCandida-Rugosa Lipaseen_US
dc.subjectImmobilizationen_US
dc.subjectAlginateen_US
dc.subjectAciden_US
dc.subjectNanoparticlesen_US
dc.subjectSupporten_US
dc.subjectOilen_US
dc.subjectOptimizationen_US
dc.subjectPurificationen_US
dc.subjectHydrolysisen_US
dc.titleEntrapment of laurel lipase in chitosan hydrogel beadsen_US
dc.typeArticleen_US

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