Entrapment of laurel lipase in chitosan hydrogel beads
Küçük Resim Yok
Tarih
2017
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Ltd
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Laurel seed lipase was entrapped within chitosan beads with ionotropic gelatin method using tripolyphosphate (TPP) as multivalent covalent counter ion. Immobilization yield was 78%. First, optimum immobilization conditions were determined, and morphology of chitosan beads was characterized by scanning electron microscopy. Optimum pH and temperature were evaluated as 6.0 and 40 degrees C, respectively. The immobilized beads saved about 55% of its activities at 60 degrees while saved about 32% at 70 degrees C for 30min. V-max/K-m values were determined as 31.75 and 2.87 using olive oil as substrate for immobilized beads and free enzyme, respectively. Immobilized beads showed the activities during 30 days at+4 degrees C.
Açıklama
Anahtar Kelimeler
Chitosan, Immobilization, Ionotropic Gelatin Method, Laurel, Lipase, Tripolyphosphate, Candida-Rugosa Lipase, Immobilization, Alginate, Acid, Nanoparticles, Support, Oil, Optimization, Purification, Hydrolysis
Kaynak
Artificial Cells Nanomedicine And Biotechnology
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
45
Sayı
5
