Histopathologic Changes in the Middle Ear Mucosa After Exposure to Pepsin and Unconjugated Bile Acid
Küçük Resim Yok
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Lippincott Williams & Wilkins
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Objective: An increasing number of studies indicate that pepsin and bile acid cause damage to the ear, nose, and throat structures as a result of extraesophageal reflux. The aim of this study was to evaluate and compare the damaging effect of bile acids and pepsin on the middle ear mucosa. Material and Methods: Twenty-nine healthy rats were included in this study. The animals were divided into 5 groups. A single daily dose of 40 mu mol/L chenodeoxycholic acid, 40 mu g/mL pepsin, and saline were injected separately into the right middle ear of the rats. On day 30, all rats were decapitated, and formalin-fixed, paraffin-embedded samples of the middle ear both from the control and experimental rats were prepared. A semiquantitative analysis was performed. Results: Inflammatory response was seen in all middle ear mucosa of rats except control group 1. The degree of inflammatory response was higher in the bile acid group when compared with the other groups. Epithelial metaplastic changes with varying number of goblet cells were observed in both the bile acid-and pepsin-injected groups. These metaplastic changes were also higher in the bile acid-induced group than in the pepsin-injected group. Conclusions: This is the first study on the middle ear mucosal damage of both pepsin and bile acid. Our results demonstrate that bile acids were associated with more extensive mucosal injury at pH 7 in comparison to pepsin in a rat animal model. Inflammatory response and metaplastic changes may play an important role in the etiology of middle ear pathologies.
Açıklama
Anahtar Kelimeler
Extraesophageal Reflux, Chenodeoxycholic Acid, Otitis Media, Mucous Cell Metaplasia, Injury, Otitis-Media, Reflux, Pathology, Pathogenesis, Bacterial, Effusion, Children, Virus, Model
Kaynak
Journal Of Craniofacial Surgery
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
25
Sayı
6
