Trypsin-catalyzed peptide synthesis in acetonitrile with low water content
Küçük Resim Yok
Tarih
2002
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Tubitak Scientific & Technological Research Council Turkey
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Trypsin was immobilized to alumina by adsorption from its aqueous solutions. The activity of trypsin was increased by immobilization for peptide synthesis. With these immobilized enzymes Bz-Arg-Leu-NH2 dipeptide synthesis was carried out. Iso amylalcohol, tetrahydrofuran and acetonitrile were used as solvents giving the highest yield in the peptide formation reaction. The yield of the peptide was strongly dependent on water content in the reaction medium. The stability of alumina-immobilized trypsin is described.
Açıklama
Anahtar Kelimeler
Peptide Synthesis Trypsin, Immobilization, Organic Solvent, Alumina, Immobilization
Kaynak
Turkish Journal Of Chemistry
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
26
Sayı
4
