EVALUATION OF BACTERIAL CYTOTOXICITY OF S. Entomophila AND B. Thuringiensis ON MEF, DU145 AND HELA CELLS

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dc.contributor.authorKazar, Gamze Altintas
dc.contributor.authorSen, Ece
dc.date.accessioned2024-06-12T10:51:55Z
dc.date.available2024-06-12T10:51:55Z
dc.date.issued2022
dc.departmentTrakya Üniversitesien_US
dc.description.abstractBacterial cancer therapy is the use of live, attenuated or genetically modified bacteria or bacterial products against cancer cells and tumors. Interest has been focused on the therapy by Clostridium spp., Salmonella spp., and Bacillus spp.. The aim of study is investigating the anti-cancer potential of B. thuringiensis and S. entomophila strains on HeLa and DU145 cancer cell lines comparing to healthy MEF cell line. B. thuringiensis and S. entomophila strains were incubated with cell lines of DU145, MEF and HeLa and the cell viability, apoptosis percentage and caspase-3 activity was assessed. B. thuringiensis decreases the viability of DU145 cells up to 40 % and MEF cells up to 60% while S. entomophila reduces the viability of cell average to 74 % and most effected cell line is found to be HeLa with the viability percentage of 59%. Apoptotic MEF and HeLa cell percentages were found to have similar values of 16% for both S. entomophila and B. thuringiensis incubation. Only different apoptotic percentage values observed were for the DU145 cell line that 6% with B. thuringiensis treatment and 23% with S. entomophila treatment. DU145 yielded exactly the same value of control sample when incubated with B. thuringiensis however S. entomophila treatment caused five-fold increase in caspase-3 activity of DU145 cells. By the study, it is found first time that S. entomophila has selective cytotoxicity to cancer cell lines of HeLa and DU145 whereas B. thuringiensis has no detectable selective cytotoxicity to cancer cell lines compared to healthy cell line.en_US
dc.description.sponsorshipScientific Research Projects Unit of Trakya University [2014/65]; OYP (Academic Member Training Program) of Trakya University; TUBITAK (The Scientific and Technological Research Council of Turkey) [BIDEB 2211-A]en_US
dc.description.sponsorshipThe authors are alone responsible for the content and writing of the paper and no ethics committee approval was needed. This study was financially supported by The Scientific Research Projects Unit of Trakya University under the project number of 2014/65 and also funded by OYP (Academic Member Training Program) of Trakya University.; Gamze Altintas Kazar was also financially supported by TUBITAK (The Scientific and Technological Research Council of Turkey) under the program of BIDEB 2211-A for PhD studies.en_US
dc.identifier.endpage4215en_US
dc.identifier.issn1018-4619
dc.identifier.issn1610-2304
dc.identifier.issue4en_US
dc.identifier.startpage4210en_US
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18535
dc.identifier.volume31en_US
dc.identifier.wosWOS:000781629900039en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherParlar Scientific Publications (P S P)en_US
dc.relation.ispartofFresenius Environmental Bulletinen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAnticancer Agentsen_US
dc.subjectApoptosisen_US
dc.subjectCaspase-3en_US
dc.subjectCell Viabilityen_US
dc.subjectBacillus Thuringiensisen_US
dc.subjectSerratia Entomophilaen_US
dc.subjectBacillus-Thuringiensisen_US
dc.subjectProteinen_US
dc.titleEVALUATION OF BACTERIAL CYTOTOXICITY OF S. Entomophila AND B. Thuringiensis ON MEF, DU145 AND HELA CELLSen_US
dc.typeArticleen_US

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