EVALUATION OF BACTERIAL CYTOTOXICITY OF S. Entomophila AND B. Thuringiensis ON MEF, DU145 AND HELA CELLS
Küçük Resim Yok
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Parlar Scientific Publications (P S P)
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Bacterial cancer therapy is the use of live, attenuated or genetically modified bacteria or bacterial products against cancer cells and tumors. Interest has been focused on the therapy by Clostridium spp., Salmonella spp., and Bacillus spp.. The aim of study is investigating the anti-cancer potential of B. thuringiensis and S. entomophila strains on HeLa and DU145 cancer cell lines comparing to healthy MEF cell line. B. thuringiensis and S. entomophila strains were incubated with cell lines of DU145, MEF and HeLa and the cell viability, apoptosis percentage and caspase-3 activity was assessed. B. thuringiensis decreases the viability of DU145 cells up to 40 % and MEF cells up to 60% while S. entomophila reduces the viability of cell average to 74 % and most effected cell line is found to be HeLa with the viability percentage of 59%. Apoptotic MEF and HeLa cell percentages were found to have similar values of 16% for both S. entomophila and B. thuringiensis incubation. Only different apoptotic percentage values observed were for the DU145 cell line that 6% with B. thuringiensis treatment and 23% with S. entomophila treatment. DU145 yielded exactly the same value of control sample when incubated with B. thuringiensis however S. entomophila treatment caused five-fold increase in caspase-3 activity of DU145 cells. By the study, it is found first time that S. entomophila has selective cytotoxicity to cancer cell lines of HeLa and DU145 whereas B. thuringiensis has no detectable selective cytotoxicity to cancer cell lines compared to healthy cell line.
Açıklama
Anahtar Kelimeler
Anticancer Agents, Apoptosis, Caspase-3, Cell Viability, Bacillus Thuringiensis, Serratia Entomophila, Bacillus-Thuringiensis, Protein
Kaynak
Fresenius Environmental Bulletin
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
31
Sayı
4
