BDE-99'un antioksidan sistem üzerine etkilerinin araştırılması
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Date
2013
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Trakya Üniversitesi Fen Bilimleri Enstitüsü
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info:eu-repo/semantics/openAccess
Abstract
2,2’,4,4’,5-pentabromodifenil eter’in (BDE-99) 0,05 mg/kg ve 0,1 mg/kg dozları mısır yağında çözülerek on gün boyunca Wistar Albino sıçanlara gavaj yoluyla verildi. Deneyin sonunda karaciğer, böbrek, ince bağırsak ve akciğer dokularında biyokimyasal ve histopatolojik değişiklikler incelendi. Buna göre; karaciğer, böbrek, ince bağırsak ve akciğerde KAT aktivitesi ve böbrekte GPX, SOD aktiviteleri istatistiksel olarak anlamlı şekilde azaldı (p<0,05). Karaciğer ve ince bağırsakta GPX aktivitesi ve karaciğerde SOD aktivitesi istatistiksel açıdan anlamlı şekilde arttı (p<0,05). Karaciğerde; ven endotel hasarı, mononuklear hücre infiltrasyonu, hepatositlerde membran hasarı, sitoplazma kaybı, hipertrofi ve genel anlamda dokuda bütünlük kaybı meydana geldiği belirlendi. Böbreklerde; Bowman kapsülünün parietal ve visseral yaprakları arasında mesafe artışı, tübülleri astarlayan epitelde membran hasarı, epitel hücrelerinde hipertrofi, nukleus ve sitoplazma kaybı gözlendi. İnce bağırsakta; epitelin, altındaki bağ dokudan ayrıldığı ve mononuklear hücre infiltrasyonu oluştuğu, akciğerde de; dokunun karakteristik süngerimsi yapısının bozulduğu, alveolar alanlarda bağ doku artışı ve mononuklear hücre infiltrasyonu meydana geldiği belirlendi.Sonuç olarak, BDE-99’un bu dozlarda antioksidan sistemi olumsuz yönde etkileyerek biyokimyasal ve histolojik değişikliklere neden olduğunu ve toksisite mekanizmasının aydınlatılması için daha detaylı çalşmalara ihtiyaç duyulduğunu düşünmekteyiz. Anahtar Kelimeler : 2,2’,4,4’,5-pentabromodifenil eter (BDE-99), SOD, GPX, KAT,karaciğer, böbrek, ince bağırsak, akciğer.
Abstract
2,2’,4,4’,5-pentabromodiphenyl ether (BDE-99) dissolved in corn oil was given to Wistar Albino rats during ten days by gavage and the biochemical and the histopathological changes in liver, kidney, small intestine and lung tissue have been examined. Accordingly GPX, SOD and CAT activity in the liver and the kidney have shown a significant change from a biochemical point (p <0.05), and CAT and GPX activity in the small Intestine showed a statistically significant change (p <0.05), and the SOD activity showed no any statistically significant change (p>0,05). CAT activity in the lungs has shown a statistically significant change (p<0.05) and GPX and SOD activities have not shown any statistically significant changes (p>0,05). And it was observed that a vein endothelial injury, mononuclear cell infiltration, membrane damage in the hepatocytes, hypertrophy, and cytoplasm loss and generally loss of integrity in the tissue also occurred in the liver. It was observed an increase in the distance (atrophy) between the parietal and visceral leaves of the Bowman's capsule in the kidneys,membrane damage in the epithelium which is lining the tubules, nucleus and cytoplasm loss in the epithelial cells, and hypertrophy. It was observed a separation of the epithelium from the connective tissue under it in the small intestine and a mononuclear cell infiltration; and in the lungs it was observed a disruption of the characteristic spongy structure of the tissue, an increase in the connective tissue in the alveolar areas and a mononuclear cell infiltration.As a result, the BDE-99 molecule, having adversely affecting the antioxidant system, causes biochemical and histological changes. Therefore we think that it is required further studies for toxicity mechanism of BDE-99 in detail.Keywords : 2,2’,4,4’,5 pentabromodiphenyl ether (BDE-99), SOD, GPX, CAT, liver, kidney, small intestine, lung.
Abstract
2,2’,4,4’,5-pentabromodiphenyl ether (BDE-99) dissolved in corn oil was given to Wistar Albino rats during ten days by gavage and the biochemical and the histopathological changes in liver, kidney, small intestine and lung tissue have been examined. Accordingly GPX, SOD and CAT activity in the liver and the kidney have shown a significant change from a biochemical point (p <0.05), and CAT and GPX activity in the small Intestine showed a statistically significant change (p <0.05), and the SOD activity showed no any statistically significant change (p>0,05). CAT activity in the lungs has shown a statistically significant change (p<0.05) and GPX and SOD activities have not shown any statistically significant changes (p>0,05). And it was observed that a vein endothelial injury, mononuclear cell infiltration, membrane damage in the hepatocytes, hypertrophy, and cytoplasm loss and generally loss of integrity in the tissue also occurred in the liver. It was observed an increase in the distance (atrophy) between the parietal and visceral leaves of the Bowman's capsule in the kidneys,membrane damage in the epithelium which is lining the tubules, nucleus and cytoplasm loss in the epithelial cells, and hypertrophy. It was observed a separation of the epithelium from the connective tissue under it in the small intestine and a mononuclear cell infiltration; and in the lungs it was observed a disruption of the characteristic spongy structure of the tissue, an increase in the connective tissue in the alveolar areas and a mononuclear cell infiltration.As a result, the BDE-99 molecule, having adversely affecting the antioxidant system, causes biochemical and histological changes. Therefore we think that it is required further studies for toxicity mechanism of BDE-99 in detail.Keywords : 2,2’,4,4’,5 pentabromodiphenyl ether (BDE-99), SOD, GPX, CAT, liver, kidney, small intestine, lung.
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Keywords
2,2’,4,4’,5-pentabromodifenil eter (BDE-99), SOD, GPX, KAT, Karaciğer, Böbrek, İnce Bağırsak, Akciğer, 2,2’,4,4’,5-Pentabromodiphenyl Ether (BDE-99), CAT, Liver, Kidney, Small intestine, Lung
