A new multienzyme-type biosensor for triglyceride determination

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dc.authorwosidÖZCAN, Hakkı/AAJ-6857-2021
dc.contributor.authorYucel, Alp
dc.contributor.authorOzcan, Hakki Mevluet
dc.contributor.authorSagiroglu, Ayten
dc.date.accessioned2024-06-12T10:51:28Z
dc.date.available2024-06-12T10:51:28Z
dc.date.issued2016
dc.departmentTrakya Üniversitesien_US
dc.description.abstractAn amperometric multienzyme biosensor for determination of triglycerides (TGs) was constructed by mounting three gelatin membrane-bound enzymes on a glassy carbon electrode (working electrode), then connecting it to electrometer along with an Ag/AgCl reference electrode and a Pt auxiliary electrode. Characterization and optimization of the multienzyme biosensor, which is prepared with glycerol kinase (GK) (E.C.2.7.1.30), glycerol-3-phosphate oxidase (GPO) (EC 1.1.3.21), and lipase (EC 3.1.1.3), were studied. In the optimization studies for the bioactive layer components of the prepared biosensor, the optimum amounts of gelatin, bovine serum albumin (BSA), and glutaraldehyde was calculated as 1mg/cm(2), 1mg/cm(2), and 2.5%, respectively. Optimum pH and temperature of the reaction of biosensor were determined as 7.0 and 40 degrees C, respectively. Linear range of triolein for the biosensor was found from the calibration curve between several substrate concentration and Current. After optimization and characterization of the biosensor, its operationability in triglycerides was also tested.en_US
dc.description.sponsorshipTrakya University Scientific Research Foundation [TUBAP-2012/195]en_US
dc.description.sponsorshipSupport from Trakya University Scientific Research Foundation (project number TUBAP-2012/195) is greatly acknowledged.en_US
dc.identifier.doi10.1080/10826068.2014.985833
dc.identifier.endpage84en_US
dc.identifier.issn1082-6068
dc.identifier.issn1532-2297
dc.identifier.issue1en_US
dc.identifier.pmid25397961en_US
dc.identifier.scopus2-s2.0-84951049122en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage78en_US
dc.identifier.urihttps://doi.org/10.1080/10826068.2014.985833
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18376
dc.identifier.volume46en_US
dc.identifier.wosWOS:000366740000012en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofPreparative Biochemistry & Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAmperometryen_US
dc.subjectGlycerol Kinaseen_US
dc.subjectGlycerol-3-Phosphate Oxidaseen_US
dc.subjectLipaseen_US
dc.subjectMultienzyme Biosensoren_US
dc.subjectTGen_US
dc.subjectSerum Triglyceridesen_US
dc.subjectConstructionen_US
dc.subjectGlycerolen_US
dc.subjectEnzymeen_US
dc.titleA new multienzyme-type biosensor for triglyceride determinationen_US
dc.typeArticleen_US

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