A new multienzyme-type biosensor for triglyceride determination
Küçük Resim Yok
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
An amperometric multienzyme biosensor for determination of triglycerides (TGs) was constructed by mounting three gelatin membrane-bound enzymes on a glassy carbon electrode (working electrode), then connecting it to electrometer along with an Ag/AgCl reference electrode and a Pt auxiliary electrode. Characterization and optimization of the multienzyme biosensor, which is prepared with glycerol kinase (GK) (E.C.2.7.1.30), glycerol-3-phosphate oxidase (GPO) (EC 1.1.3.21), and lipase (EC 3.1.1.3), were studied. In the optimization studies for the bioactive layer components of the prepared biosensor, the optimum amounts of gelatin, bovine serum albumin (BSA), and glutaraldehyde was calculated as 1mg/cm(2), 1mg/cm(2), and 2.5%, respectively. Optimum pH and temperature of the reaction of biosensor were determined as 7.0 and 40 degrees C, respectively. Linear range of triolein for the biosensor was found from the calibration curve between several substrate concentration and Current. After optimization and characterization of the biosensor, its operationability in triglycerides was also tested.
Açıklama
Anahtar Kelimeler
Amperometry, Glycerol Kinase, Glycerol-3-Phosphate Oxidase, Lipase, Multienzyme Biosensor, TG, Serum Triglycerides, Construction, Glycerol, Enzyme
Kaynak
Preparative Biochemistry & Biotechnology
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
46
Sayı
1
