İki farklı fermentasyon tekniği ile Aspergillus sclerotiorum'dan amilaz üretrimi ve nişasta hidrolizinde kullanılması
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Dosyalar
Tarih
2007
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Trakya Üniversitesi Fen Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada Aspergillus sclerotiorum α-amilazının SSF ve SmF'de optimum üretim şartları belirlenmiş ve nişasta hidrolizi çalışılmıştır. SSF'de üretilen Aspergillus sclerotiorum α-amilaz aktivitesi için optimum üretim şartları araştırıldı. Optimum nem miktarı % 85, üretim süresi 6. gün, aşı miktarı 2 ml, substrat miktarı 5 gr, başlangıç pH'sı 5 olarak belirlendi. SSF'deki katabolit represyon çalışmasında ise glukoz miktarının α-amilaz aktivitesini etkilemediği görüldü. SSF'de kalan glukoz miktarı incelendiğinde 0 mg/ml, 2,5 mg/ml, 5 mg/ml glukoz içeren ortamlarda 3.günde, 10 mg/ml ve 50 mg/ml içeren ortamda ise 5.günde ortamda tamamen tükendiği görüldü. SmF'de üretilen Aspergillus sclerotiorum α-amilaz aktivitesi için optimum üretim şartları araştırıldığında, optimum üretim süresi 7. gün, başlangıç pH'sı 4, aşı miktarı 1,5 ml olarak belirlendi. SmF'deki katabolit represyon çalışmasında ise glukoz miktarının α-amilaz aktivitesini etkilediği görüldü. SmF'de kalan glukoz miktarı incelendiğinde ise 0 mg/ml glukoz içeren ortamda 1. günde, 2,5mg/ml, 5 mg/ml, 10 mg/ml glukoz içerende 3. günde ve 50 mg/ml glukoz içeren ortamda 5. günde halen daha glukoz varlığı gözlendi. SSF'de üretilen enzim preparatının nişasta hidrolizi gerçekleştirildi ve hidroliz ürünlerinin ince tabaka kromotografisi ile kalitatif analizi yapıldı. 120. dakikada hidroliz yüzdesi %98,91 olarak belirlendi. Hidroliz ürünleri ise maltoz, tanımlanamayan oligosakkaritler ve glukozdu.
In this study it was determined the conditions of optimum production of Aspergillus sclerotiorum α-amylase in SSF and SmF, and studied starch hydrolysis. When investigated the conditions of optimum production of Aspergillus sclerotiorum α-amylase in SSF, it was found that the production period was 6th day, amount of inoculation was 2 ml, amount of substrate was 5 gr, level of moisture was 85 %, initial pH was 5. It was studied catabolite repression in SSF, and discovered that α-amylase production wasn?t effected by amount of glucose. When investigated the residual amount of glucose in SSF it was seen that glucose was totally dissappeared at 3rd day in mediums containing 0 mg/ml, 2,5 mg/ml, 5 mg/ml glucose and at 5th days in the mediums containing 10 mg/ml, 50 mg/ml glucose When studied the conditions of optimal production of Aspergillus sclerotiorum α-amylase in SmF, it was found that the production period was 7th day, amount of inoculation was 1,5 ml, initial pH was 4. It was studied catabolite repression in SmF. It was discovered that α-amylase production was effected by amount of glucose. When investigated the residual amount of glucose in SmF it was seen that glucose was totally dissappeared at 3rd day in mediums containing 0 mg/ml, 2,5 mg/ml, 5 mg/ml, 10 mg/ml glucose and at 5th days in the mediums containing 50 mg/ml glucose It was carried out the starch hydrolysis of Aspergillus sclerotiorum α-amylase produced in SSF, and studied the qualitative analysis of the hydrolysis products with thin layer chromatography. After 120 minutes, the hidrolysis percentage was found as 98,91 %. Hydrolysis products were mainly maltose, unidentified oligosaccharides, and glucose.
In this study it was determined the conditions of optimum production of Aspergillus sclerotiorum α-amylase in SSF and SmF, and studied starch hydrolysis. When investigated the conditions of optimum production of Aspergillus sclerotiorum α-amylase in SSF, it was found that the production period was 6th day, amount of inoculation was 2 ml, amount of substrate was 5 gr, level of moisture was 85 %, initial pH was 5. It was studied catabolite repression in SSF, and discovered that α-amylase production wasn?t effected by amount of glucose. When investigated the residual amount of glucose in SSF it was seen that glucose was totally dissappeared at 3rd day in mediums containing 0 mg/ml, 2,5 mg/ml, 5 mg/ml glucose and at 5th days in the mediums containing 10 mg/ml, 50 mg/ml glucose When studied the conditions of optimal production of Aspergillus sclerotiorum α-amylase in SmF, it was found that the production period was 7th day, amount of inoculation was 1,5 ml, initial pH was 4. It was studied catabolite repression in SmF. It was discovered that α-amylase production was effected by amount of glucose. When investigated the residual amount of glucose in SmF it was seen that glucose was totally dissappeared at 3rd day in mediums containing 0 mg/ml, 2,5 mg/ml, 5 mg/ml, 10 mg/ml glucose and at 5th days in the mediums containing 50 mg/ml glucose It was carried out the starch hydrolysis of Aspergillus sclerotiorum α-amylase produced in SSF, and studied the qualitative analysis of the hydrolysis products with thin layer chromatography. After 120 minutes, the hidrolysis percentage was found as 98,91 %. Hydrolysis products were mainly maltose, unidentified oligosaccharides, and glucose.
Açıklama
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Anahtar Kelimeler
Biyoloji, Biology
