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Öğe Cytotoxic Activities of Certain Medicinal Plants on Different Cancer Cell Lines(Turkish Pharmacists Assoc, 2017) Ugur, Deniz; Gunes, Hatice; Gunes, Fatma; Mammadov, RamazanObjectives: In recent years, the use of plants for the prevention and treatment of cancer is gaining more attention due to their diverse range of phytochemical constituents and fewer adverse effects. In this study, four medicinal plant species from the Kars province of Turkey were investigated for their cytotoxic potential against six different cancer cell lines and one normal cell line. Materials and Methods: MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-dipenyltetrazolium bromide] assay was performed to assess cytotoxic activity and apoptotic effect was determined using flow cytometry and caspase-3 analyses. Results: Significant cytotoxicity (>= 70%) was observed with the leaf extract of Artemisia absinthium on A-549, CCC-221, K-562, MCF-7, PC-3 cells,whereas seed extracts caused significant cytotoxicity (>= 70%) on CCC-221, K-562, MCF-7, PC-3 cells. Selective cytotoxicity was obtained with leaf extract on A-549 and K-562 cells; and with seed extract on K-562, MCF-7 and PC-3 cells compared with normal Beas-2B cells. The levels of cytotoxicity for both extracts were time-and dose-dependent at lower concentrations. Moreover, selective cytotoxicity (78%) was detected on A-549 cells with the seed extract of Plantago major. Cytotoxicity of extracts from Hyoscyamus niger and Amaranthus retrosa ranged between 10% and 30%. Conclusion: A. absinthium extracts and P. major seed extract have potential for development as therapeutic agents for cytotoxicity on certain cancer cells following further investigation.Öğe Food Plants Used in Meric Town from Turkey(Assoc Pharmaceutical Teachers India, 2017) Gunes, FatmaBackground: Local people are using the plants those are growing naturally in proximity for many purposes, for example pharmaceutical, food, decoration, and ornamental. In recent years, the number of scientists who are interested in ethnobotany has increased. Aim: The aim of this study was to determine the plants used for food by the local people in the province of Meric and its villages. Methods: 16 villages were visited and interviews were performed with elderly people of the villages, 38 persons in total, in various places. Interviews were carried out face-to-face with the community. Results: At the end of the field studies, food plants from 45 taxa including 27 families were recorded. The herbarium numbers, latin name of the plants, local names, families, village number, parts used and form of usage were listed alphabetically in the table. Conclusion: This is the first ethno botanical study conducted to determine the local names and uses of food plants in Meric town.Öğe Medicinal Plants Used in Meric Town from Turkey(Assoc Pharmaceutical Teachers India, 2017) Kartal, Ciler; Gunes, FatmaBackground: There are a few studies on medical plants used in the Trakya region of Turkey ( Havsa, Lalapasa, Uzunkopru, Ipsala, Enez, Kirklareli). However, there has been no research study performed investigating the preparation and medicinal uses of wild plants in Meric town. Aim: The aim of this study is to determine the parts of locally growing medicinal plants used by local people in Meric town and the purpose of their use. Methods: In this study, 16 villages in Meric town (Edirne province, Turkey) were visited, and interviews were performed with 38 persons in total. Results: As result of the study, 24 plant taxa in 19 families were recorded as medicinal plants used by local people. Conclusion: These traditional medicinal plants have been mostly used for the treatment of diabetes, stomach ailments, hemorrhoids, rheumatism and asthma.Öğe Medicinal plants used in the Uzunkopru district of Edirne, Turkey(Polskie Towarzystwo Botaniczne, 2017) Gunes, FatmaThis study examined the use of plants in Uzunkopru and surrounding villages in the years 2013-2015 during the flowering and fruiting season of the studied plants (March-October). Interviews were carried out face-to-face with members of the community. Fifty-seven people in 55 villages were interviewed. Overall, medicinal plants from 96 taxa belonging to 45 families were recorded. Traditional medicinal plants were used to treat 80 diseases and ailments such as diabetes, cold, flu, cough, stomachache, and hemorrhoids. According to the results, the largest eight families are Rosaceae, Lamiaceae, Asteraceae, Poaceae, Ranunculaceae, Malvaceae, Cucurbitaceae, and Brassicaceae. The most commonly used species were Anthemis cretica subsp. tenuiloba, Cotinus coggyria, Datura stramonium, Ecballium elaterium, Hypericum perforatum, Prunus spinosa, Pyrus elaeagnifolia subsp. bulgarica, Rosa canina, Sambucus ebulus, Tribulus terestris, Urtica dioica. The herbarium numbers, Latin and local names, families, village numbers, parts used, usage forms, and uses were listed alphabetically in a table. In this study, 219 local names were identified.Öğe Morphological, anatomical and karyological investigations of the Turkish endemic species Lathyrus woronowii Bornm. (Fabaceae)(Univ Zagreb, Fac Science, Div Biology, 2017) Gunes, Fatma; Meric, CilerLathyrus woronowii Bornm., an endemic species of Turkey, is threatened with extinction due to dam construction. It exists only in the Coruh valley, Artvin. This annual species is in the critically endangered (CR) category according to the International Union of Conservation of Nature (IUCN) criteria. Its morphology, anatomy and karyology are studied here for the first time. A detailed description is given and the general appearance of the species has been drawn; cross sections from the stem and leaf have been taken and examined; and the diploid chromosome number (2n = 14) has been reported and illustrated for the first time.Öğe Pollen morphology of Lathyrus (Leguminosae) taxa belonging to Lathyrus, Orobastrum and Cicercula sections from Turkey(Springer Wien, 2012) Gunes, FatmaThe pollen morphology of 20 wild taxa belonging to Lathyrus (Syn: Eulathyrus), Orobastrum (Taub.) Boiss. and Cicercula (Medic.) Gren. & Godr. sections of Lathyrus L. grown in Turkey (L. rotundifolius Wild. subsp. miniatus (Bieb. ex Steven) P.H. Davis, L. grandiflorus Sibth. & Sm., L. saxatilis (Vent.) Vis., L. vinealis Boiss. & NoA <<, L. inconspicuus L. var. inconspicuus, L. inconspicuus var. stenophyllus (Boiss.) Rech. f., L. tauricola P.H. Davis, L. woronowii Bornm., L. hierosolymitanus Boiss., L. cassius Boiss., L. gorgoni Parl. var. gorgoni, L. pseudo-cicera Pamp., L. sativus L., L. blepharicarpus Boiss., L. stenophyllus Boiss. & Heldr., L. belinensis Maxted & Goyder, L. phaselitanus Hub.-Mor. & P.H.Davis, L. chrysanthus Boiss., L. chloranthus Boiss., and L. trachycarpus (Boiss.) Boiss were examined by light microscopy (LM) and scanning electron microscopy (SEM) in this study. The pollen grains were 3-zonocolporate, spheroidal, subprolate, and prolate (P/E = 0.99-1.48) types, and were medium in size (equatorial view: rectangular or elliptical-obtuse-convex; polar view: circular, triangular and quinquangular-obtuse-convex). The smallest pollen grains belonged to L. tauricola (P = 30.94/E = 31.20) and the largest to L. grandiflorus (P = 50.60/E = 36.40). The ornamentation was reticulate and reticulate-granulate in the mesocolpium, and usually psilate in the apocolpium. Some photographs included in this work were taken using both LM and SEM.Öğe Seed characteristics and testa textures of Pratensis, Orobon, Lathyrus, Orobastrum and Cicercula sections from Lathyrus (Fabaceae) in Turkey(Springer Wien, 2013) Gunes, FatmaThe seed morphologies and testa textures of 23 taxa belonging to the Pratensis, Orobon, Lathyrus, Orobastrum and Cicercula sections of Lathyrus that can widely be found in Turkey were analysed. The findings obtained in this study and previous findings (34 taxa in total) were compared and interpreted at the level of the sections. Morphological properties including seed size, general shape, surface shape, colour, hilum length and width were measured under stereomicroscopy. The seeds were of spheroidal, subprolate and prolate (P/E = 0.90-1.58) types and medium in size. The smallest seeds belonged to Lathyrus inconspicuus var. inconspicuus (P = 2.19 +/- A 0.25 mm, E = 2.08 +/- A 0.16 mm) and the largest to L. sativus (P = 5.88 +/- A 0.74 mm, E = 5.36 +/- A 0.57 mm). The smallest hilum belonged to L. inconspicuus var. stenophyllus (0.38 +/- A 0.04 mm) and the largest to L. sylvestris (4.50 +/- A 0.58 mm). Testa textures such as papillae shape, dense, ribbing and presence or absence of a waxy layer were examined using scanning electron microscopy (SEM). In addition, some photographs included in this study were taken via stereomicroscopy and SEM.