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    Characterization of Calpastatin Gene in Purebred and Crossbred Turkish Grey Steppe Cattle
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2013) Kok, Suleyman; Atalay, Sertac; Savasci, Mustafa; Eken, Hasan Semih
    Calpastatin (CAST) is a specific inhibitor of calcium-dependent neutral protease mu-calpain found in mammalian tissues. The genetic variants in the bovine CAST gene were analysed by using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The animal material of the study consisted of 132 bulls and heifers in Turkish Grey steppe cattle and Turkish Grey steppe x Brown Swiss crossbreds. C and G alleles are characterized by CAST/RsaI polymorphism were detected in the sample of animals were studied. Allele frequency C was significantly frequent in the crossbred group compared to the pure Turkish Grey steppe animals (P<0.05). In the total samples of animals, the average allele frequency C was 56.1%. Genotypic frequencies were estimated as 0.257, 0.499 and 0.243 in the purebred Turkish Grey steppe, and 0.388, 0.470 and 0.142 in the Turkish Grey steppe x Brown Swiss crossbred population for genotypes CC, CG and GG, respectively. As a result, genotypic distributions were equilibrium in both pure and crossbred examples of Turkish Grey steppe cattle.
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    The Genetic Characterization of Turkish Grey Cattle with Regard to UoG Cast, CAPN1 316 and CAPN1 4751 Markers
    (Zoological Soc Pakistan, 2017) Kok, Siileyman; Atalay, Sertac; Eken, Hasan Semih; Savasci, Mustafa
    Turkish Grey cattle (TGC) is one of Turkey's native cattle breeds TGC was a popular race to breeding within Thrace and Western Anatolia, but unfortunately they are under the threat of extinction recently The application of gene specific molecular markers in genotypmg and genetic identification is of essential significance for preservmg genetic diversity The aim of this study was to reveal the genotype profile of indigenous types for TGC breed In order to characterize TGC population, two polymorphic loci in cattle Calpain gene (CAPN1) and one polymorphic locus in Calpastatm gene CAST were studied In order to sort the allele variants (G/C), PCR products, the ones in CAST with RsaI enzyme, was cut using RFLP method. The allele variants (G/C) of CAPN1 316 and (C/T) of CAPN1 4751 were determined with ARMS-PCR method, This study was conducted to determme three SNPs which were related to meat quality in 130 purebred female and male samples of TGC breed In TGC samples, C allele frequencies related to UoG-CAST, CAPN1 316 and CAPN1 4751 polymorphisms were found to be 0.6038, 0 1115 and 0.5654, respectively. CC genotype frequencies in the three polymorphic areas m TGC were calculated as 0.365, 0 012 and 0 320, respectively, while the distributions of the marker genotypes, except for CAPN1 4751 were found to be significant (P<0.05). The positive alleles of SNPs in Calpam and Calpastatin genes and all of their genotype combinations, were also found to be carried by TGC