The Genetic Characterization of Turkish Grey Cattle with Regard to UoG Cast, CAPN1 316 and CAPN1 4751 Markers
Küçük Resim Yok
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Zoological Soc Pakistan
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Turkish Grey cattle (TGC) is one of Turkey's native cattle breeds TGC was a popular race to breeding within Thrace and Western Anatolia, but unfortunately they are under the threat of extinction recently The application of gene specific molecular markers in genotypmg and genetic identification is of essential significance for preservmg genetic diversity The aim of this study was to reveal the genotype profile of indigenous types for TGC breed In order to characterize TGC population, two polymorphic loci in cattle Calpain gene (CAPN1) and one polymorphic locus in Calpastatm gene CAST were studied In order to sort the allele variants (G/C), PCR products, the ones in CAST with RsaI enzyme, was cut using RFLP method. The allele variants (G/C) of CAPN1 316 and (C/T) of CAPN1 4751 were determined with ARMS-PCR method, This study was conducted to determme three SNPs which were related to meat quality in 130 purebred female and male samples of TGC breed In TGC samples, C allele frequencies related to UoG-CAST, CAPN1 316 and CAPN1 4751 polymorphisms were found to be 0.6038, 0 1115 and 0.5654, respectively. CC genotype frequencies in the three polymorphic areas m TGC were calculated as 0.365, 0 012 and 0 320, respectively, while the distributions of the marker genotypes, except for CAPN1 4751 were found to be significant (P<0.05). The positive alleles of SNPs in Calpam and Calpastatin genes and all of their genotype combinations, were also found to be carried by TGC
Açıklama
Anahtar Kelimeler
Single-Nucleotide Polymorphisms, Fragment-Length-Polymorphisms, Meat Quality Traits, Beef-Cattle, Bos-Indicus, Calpastatin Gene, Candidate Genes, M. Longissimus, Association, Tenderness
Kaynak
Pakistan Journal Of Zoology
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
49
Sayı
1
