Yazar "Atalay, Sertac" seçeneğine göre listele

Listeleniyor 1 - 4 / 4
  • Küçük Resim Yok
    Öğe
    Characterization of Calpastatin Gene in Purebred and Crossbred Turkish Grey Steppe Cattle
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2013) Kok, Suleyman; Atalay, Sertac; Savasci, Mustafa; Eken, Hasan Semih
    Calpastatin (CAST) is a specific inhibitor of calcium-dependent neutral protease mu-calpain found in mammalian tissues. The genetic variants in the bovine CAST gene were analysed by using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The animal material of the study consisted of 132 bulls and heifers in Turkish Grey steppe cattle and Turkish Grey steppe x Brown Swiss crossbreds. C and G alleles are characterized by CAST/RsaI polymorphism were detected in the sample of animals were studied. Allele frequency C was significantly frequent in the crossbred group compared to the pure Turkish Grey steppe animals (P<0.05). In the total samples of animals, the average allele frequency C was 56.1%. Genotypic frequencies were estimated as 0.257, 0.499 and 0.243 in the purebred Turkish Grey steppe, and 0.388, 0.470 and 0.142 in the Turkish Grey steppe x Brown Swiss crossbred population for genotypes CC, CG and GG, respectively. As a result, genotypic distributions were equilibrium in both pure and crossbred examples of Turkish Grey steppe cattle.
  • Küçük Resim Yok
    Öğe
    The effects of polymorphisms in the CX3CR1 gene on the development of canine hip dysplasia
    (Tubitak Scientific & Technological Research Council Turkey, 2022) Atalay, Sertac; Kok, Suleyman
    Hip dysplasia, caused by both environmental and genetic components, is a common disorder characterized by hip instability in humans and dogs. Unfortunately, the genetic mechanisms that cause the disease in both have not been fully determined. The aim of this study was polymorphisms in the exon 2 and 3' UTR regions of the CX3CR1 gene were determined and their effects on the development of Canine Hip Dysplasia (CHD) in three dog breeds (German Shepherd, Belgian Malinois, Labrador Retriever). For this purpose, a case -control study was designed with 172 dogs in Dog Breeding and Training Center (DBTC) in Turkey. Each dog was evaluated according to the Norberg angle by the DBTC veterinarians. One hundred and seventeen dogs (32 German Shepherds, 49 Belgian Malinois, 36 Labrador Retrievers) classified as normal were included in the control group, and fifty - five dogs (24 German Shepherds, 14 Belgian Malinois, 17 Labrador Retrievers) diagnosed with CHD were included in the case group. Molecular genetic analyzes were performed with blood samples taken from each dog. Seven previously identified SNPs (g.8938599_8938600insCC, g.8937121G>A, g.8937137A>G, g.8937319T>G, g.8937441T>C, g.8937450A>G, g.8937590C>T) and a rare novel deletion (g. 8937205_ 8937206del) were identified in the 3' UTR regions of the CX3CR1 gene. The distribution of SNPs alleles in the case and control was compared by means of statistical analysis at allelic, genotypic, haplotypic, and SNP - SNP interaction levels. Single SNP analysis revealed that g.8937121G>A was significantly associated with susceptibility to CHD in Belgian Malinois (p = 0.00049) in the codominant model. Five SNP - SNP interactions were identified to be associated with CHD in Labrador Retrievers and the most suggestive of these was between g.8938599_8938600insCC and g.8937450A>G (p = 0.0004). We found that one haplotype block, consisting of two SNPs (g.8937137A>G and g.8937319T>G) was associated with susceptibility to CHD in Belgian Malinois (p = 0.022). None of the detected polymorphisms was statistically significantly associated with CHD in German Shepherds.
  • Küçük Resim Yok
    Öğe
    The Genetic Characterization of Turkish Grey Cattle with Regard to UoG Cast, CAPN1 316 and CAPN1 4751 Markers
    (Zoological Soc Pakistan, 2017) Kok, Siileyman; Atalay, Sertac; Eken, Hasan Semih; Savasci, Mustafa
    Turkish Grey cattle (TGC) is one of Turkey's native cattle breeds TGC was a popular race to breeding within Thrace and Western Anatolia, but unfortunately they are under the threat of extinction recently The application of gene specific molecular markers in genotypmg and genetic identification is of essential significance for preservmg genetic diversity The aim of this study was to reveal the genotype profile of indigenous types for TGC breed In order to characterize TGC population, two polymorphic loci in cattle Calpain gene (CAPN1) and one polymorphic locus in Calpastatm gene CAST were studied In order to sort the allele variants (G/C), PCR products, the ones in CAST with RsaI enzyme, was cut using RFLP method. The allele variants (G/C) of CAPN1 316 and (C/T) of CAPN1 4751 were determined with ARMS-PCR method, This study was conducted to determme three SNPs which were related to meat quality in 130 purebred female and male samples of TGC breed In TGC samples, C allele frequencies related to UoG-CAST, CAPN1 316 and CAPN1 4751 polymorphisms were found to be 0.6038, 0 1115 and 0.5654, respectively. CC genotype frequencies in the three polymorphic areas m TGC were calculated as 0.365, 0 012 and 0 320, respectively, while the distributions of the marker genotypes, except for CAPN1 4751 were found to be significant (P<0.05). The positive alleles of SNPs in Calpam and Calpastatin genes and all of their genotype combinations, were also found to be carried by TGC
  • Küçük Resim Yok
    Öğe
    The Use of Various SNPs in CAST and CAPN1 Genes to Determine the Meat Tenderness in Turkish Grey Cattle
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2018) Kok, Suleyman; Atalay, Sertac
    The aim of this study is to investigate the relationship between some genotypic characteristics of purebred Turkish Grey Cattle (TGC) and beef tenderness characteristics. There is a linear relationship between meat tenderness and the increasing calcium activity after slaughtering. Calpastatin (CAST) is a neutral protease inhibitor of Calpain (CAPN1) in mammalian tissues. The three polymorphic places in the CAST and the CAPN1 genes in cattle (UoG-CAST, CAPN1 316 and 4751) are known as the markers of beef quality. The relationship between the tenderness traits of the longissimus dorsi (LD) and the 3 beef quality markers (3 SNPs) in pure TGC have been investigated and discussed. PCR-RFLP and ARMS-PCR methods were utilized to identify the genotypes. In order to determine the water holding capacity (WHC), cooking loss (CL) and the shear force (SF), samples extracted from LD were probed. The average and standard error SF of a two-year-old TCG bred in extensive conditions was 4.339 +/- 0.217 kg/cm(2) for the heifers and 4.689 +/- 0.569 kg/cm(2) for bulls. Both alleles of the UoG-CAST (C/G), the CAPN1 316 (C/G) and the CAPN1 4751(C/T) polymorphisms in the samples were observed. The average SF of 3.943 +/- 0.441 kg/cm(2), 4.537 +/- 1.666 kg/cm(2), and 3.869 +/- 0.721 kg/cm(2) were used for the CAST-CC, the CAPN1 4751-CC and the CAPN1 316-GC, respectively in order to cut the muscle fibers of the genotypes that have a positive effect on tenderness. No cattle of the CAPN1 316-CC genotype was identified among the samples. The average and standart error WHC, CL and SF values for the entire sample including heifers and bulls were found as 11.693 +/- 0.761%, 26.952 +/- 0.636%, and 4.483 +/- 0.252 kg/cm(2), respectively. In conclusion, the presence of genetic variation in specific SNP markers of beef tenderness in purebred TGC can contribute to the process of raising TGC with more tender meat.