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    Development of a reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of Amasya cherry disease
    (Wiley-Blackwell Publishing, Inc, 2007) Kozlakidis, Z.; Citir, A.; Acikgoz, S.; Coutts, R. H. A.
    A reverse transcription-polymerase chain reaction (RT-PCR) procedure was developed to detect Amasya cherry disease (ACD) in naturally infected sweet cherry (Prunus avium) leaves sampled from Turkey. The procedure was based on detection of the presence of a mycoviral-like double-stranded RNA (dsRNA) of 5.3 kbp always found in association with ACD, which is probably caused by a fungus. Specific primers were designed to amplify a fragment of the diagnostic dsRNA. The method will improve routine diagnosis of ACD in Prunus spp.
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    Molecular characterization of the largest mycoviral-like double-stranded RNAs associated with Amasya cherry disease, a disease of presumed fungal aetiology
    (Microbiology Soc, 2006) Kozlakidis, Z.; Covelli, L.; Di Serio, F.; Citir, A.; Acikgoz, S.; Hernandez, C.; Ragozzino, A.
    The sequence of the four large Q double-stranded RNAs (dsRNAs) associated with Amasya cherry disease (ACD), which has a presumed fungal aetiology, is reported. ACID L dsRNAs 1 (5121 bp) and 2 (5047 bp) potentially encode proteins of 1628 and 1620 aa, respectively, that are 37 % identical and of unknown function. ACID L dsRNAs 3 (4458 bp) and 4 (4303 bp) potentially encode proteins that are 68 % identical and contain the eight motifs conserved in RNA-dependent RNA polymerases (RdRp) of dsRNA mycoviruses, having highest similarity with those of members of the family Totiviridae. Both terminal regions share extensive conservation in all four RNAs, suggesting a functional relationship between them. As ACID L dsRNAs 1 and 2 do not encode RdRps, both are probably replicated by those from either ACID L dsRNA 3 or 4. Partial characterization of the equivalent L dsRNAs 3 and 4 associated with cherry chlorotic rusty spot revealed essentially identical sequences.