Eruygur, NuraniyeAtas, MehmetTekin, MehmetCevik, Ozge2024-06-122024-06-1220232459-1459https://doi.org/10.33808/clinexphealthsci.1058614https://search.trdizin.gov.tr/yayin/detay/1196401https://hdl.handle.net/20.500.14551/17980Objective: The Achillea species have been used to treat various ailments due to its anti-inflammatory, hemostatic, spasmolytic and cholagogue effects in the Turkish traditional medicine. However, there is no biological activity studies on some Achillea species except for the well-knowns. This work aimed to determine the antioxidant, antimicrobial and cytotoxic activities of the crude ethanolic extracts and fractions of Achillea sintenisii using in vitro methods. Methods: The antioxidant activity was investigated by DPPH (1,1'-diphenyl - 2-picrylhydrazyl), ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging, total phenol and total flavonoid content, and iron chelating methods. Antimicrobial activity evaluated by micro-plate dilution method against five test organisms. Cytotoxicity was determined by MTT method using MCF-7 breast cancer cell line and PC-3 prostate cancer cell line. Apoptosis was also measured by AO/EB staining. Results: The n-Hexane fractions showed the highest antimicrobial and cytotoxic activities, respectively. Administration of the extracts on the cancer cells showed a concentration dependent inhibition on cell proliferation. The anti-proliferation effect could be via apoptosis and associated with the cell death. Conclusion: The results showed that the extracts demonstrated antioxidant, antimicrobial, and cytotoxic activity, also supports the claims of traditional usage.en10.33808/clinexphealthsci.1058614info:eu-repo/semantics/openAccessAchillea SintenisiiAntioxidantMicrodilutionCytotoxicityKoch Essential OilChemical-CompositionBiological-ActivitiesMethanol ExtractsL.TeretifoliaAnticholinesteraseConstituentsMillefoliumLycaonicaArticle133517524N/AWOS:0011533766000111196401