Cevik, DicleErdogan, SuatSerttas, RizaKan, YukselKirmizibekmez, Hasan2024-06-122024-06-1220231612-18721612-1880https://doi.org/10.1002/cbdv.202200589https://hdl.handle.net/20.500.14551/21391Cytotoxic activity-guided fractionation studies on Glycyrrhiza echinata roots led to the isolation of eight compounds (1-8). Chemical structures of the isolates were identified by NMR and MS analysis. Among the tested molecules, retrochalcones namely echinatin (3) (IC50=23.45-41.83 mu M), licochalcone B (4) (IC50=36.04-39.53 mu M) and tetrahydroxylmethoxychalcone (5) (IC50=7.09-80.81 mu M) were the most active ones against PC3, MCF7 and HepG2 cells. Moreover, 5 exhibited selectivity on prostate cancer cells (SI: 5.19). Hoechst staining and Annexin V/PI binding assays as well as cell cycle analysis on the compounds 3 (23 mu M) and 5 (5 and 7 mu M) demonstrated that these retrochalcones induced apoptosis and significantly suppressed cell cycle in G(1) and G(2)/M phases. Furthermore, 3 and 5 showed antimigratory effects on PC3 cells by wound healing assay. The results indicated that tested retrochalcones most particularly 5 could be potential anticancer drug candidates that prevent proliferation and migration of cancer cells.en10.1002/cbdv.202200589info:eu-repo/semantics/closedAccessGlycyrrhiza EchinataBioassay-Guided IsolationRetrochalconeCytotoxic ActivityApoptosisFlavonoidsMechanismsApoptosisChalconeLicoriceArticle201Q3WOS:0008968394000012-s2.0-8514377384836448364Q2