Hatipoglu, ARTemiz, EYüksel, MHoscoskun, ZCoskun, IHüseyinova, G2024-06-122024-06-1220010172-6390https://hdl.handle.net/20.500.14551/21553Background/Aims: We investigated the role of the electron microscopy and hepatobiliary scintigraphy in determining the effect of DMSO (dimethysulphoxide) and ischemia/reperfusion injury in the liver after the Pringle maneuver. Methodology: Twenty-four rabbits were divided into the following groups; A: Control group, B: Pringle, C: 10mg/kg DMSO, D: 1g/kg DMSO + Pringle. Group A was considered as a control group and only laparotomy was applied. Group B was exposed to Pringle maneuver only. Group C:was given 10mg/kg of DMSO via the vena cava inferior. Group D was given 1g/kg of DMSO- A clamp was fastened for the groups of B, C and D in the 30th minute of the Pringle maneuver and a biopsy was applied five minutes later. Fifteen minutes later a dynamic hepatobiliary scintigraphy was applied. From dynamic images, liver peak time and activity half time of the liver were obtained. Results: It was found that liver peak time and liver activity half time values of the group B, C and D were significantly longer than group A. Liver peak time and liver activity half time values of group B was not different from group C. However, some values of group D were found to be significantly shorter than groups B and C. In the electron microscopy examination, only in group B were some specific degenerative changes observed in the sinusoids. We observed less irreversible changes in group C than in group B. Oh the other hand, the least irreversible changes were in group D. Conclusions: As a conclusion, while electron microscopy is regarded as the gold standard, hepatobiliary scintigraphy may be thought of as an easily applicable: method in determining the ischemic reperfusion injury in the clinical comparison of the protective agents.eninfo:eu-repo/semantics/closedAccessHepatobiliary ScintigraphyDMSOElectron MicroscopyIschemia/Reperfusion InjuryFree-Radical ScavengersIschemia-ReperfusionDimethyl-SulfoxideLiver IschemiaSuperoxide-DismutaseLipid-PeroxidationOxygen RadicalsRat-LiverMetabolismDimethylsulfoxideArticle4839798802Q2WOS:0001696316000452-s2.0-0034930777N/A