Ertan, FigenBalkan, BilalBalkan, SedaAktac, Tulin2024-06-122024-06-1220060006-30881336-9563https://doi.org/10.2478/s11756-006-0137-2https://hdl.handle.net/20.500.14551/18315Production of a-amylase from local isolate, Penicillium chrysogenum, under solid-state fermentation (SSF) was carried out in this study. Different agricultural by-products, such as wheat bran (WB), sunflower oil meal (SOM), and sugar beet oil cake (SBOC), were used as individual substrate for the enzyme production. WB showed the highest enzyme activity (750 U/gds). Combination of WB, SOM, and SBOC (1:3:1 w/w/w) resulted in a higher enzyme yield (845 U/gds) in comparison with the use of the individual substrate. This combination was used as mixed solid substrate for the production of a-amylase from P. chrysogenum by SSF. Fermentation conditions were optimized. Maximum enzyme yield (891 U/gds) was obtained when SSF was carried out using WB + SOM + SBOC (1:3:1 w/w/w), having initial moisture of 75 %, inoculum level of 20 %, incubation period of 7 days at 30 degrees C. Galactose (1 % w/w), urea and peptone (1 % w/w), as additives, caused increase in the enzyme activity.en10.2478/s11756-006-0137-2info:eu-repo/semantics/closedAccessAlpha-AmylasePenicillium ChrysogenumSolid-State FermentationWheat BranSunflower Oil MealSugar Beet Oil CakeAspergillus-OryzaeStarchBiosynthesisGlucoamylaseHydrolysisCultureWasteNigerArticle616657661Q4WOS:0002446444000032-s2.0-33947165190Q3